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The map label for this gene is cysW [H]

Identifier: 117921629

GI number: 117921629

Start: 3829121

End: 3829990

Strand: Direct

Name: cysW [H]

Synonym: Shewana3_3191

Alternate gene names: 117921629

Gene position: 3829121-3829990 (Clockwise)

Preceding gene: 117921628

Following gene: 117921630

Centisome position: 77.01

GC content: 51.72

Gene sequence:

>870_bases
ATGAACTCATTTAAACCGTTAAGAGTCGGTGAAGCGCCCCTTATCAAATGGAGCCTAATCACCCTAGCCGTGTTTTTGGC
CATGGTATTGTTGCTACTGCCCTTAGTCAGTATTTTCCAGCAGGCCTTCGTCGGTGGTTGGGAGCGTTATATCGAGCATC
TTAGCCAGCCCGACTCACTCCACGCTATCGGGCTGACCCTTATGGTCGCAGCGCTGACCGTGCCAATTAACTTAGTGTTT
GGGGTGCTGCTCGCTTGGAGCGTAACGCGCTTTGAGTTTCCGGGGCGTAAGTTACTTATCACCTTGATTGATATTCCCTT
TGCCGTATCGCCCGTGGTCGCAGGCTTACTCTATCTGCTGCTTTACGGCAACAGTGGCTGGCTTGGCGCTTGGCTATTTG
AGCACGATTTACAGATCATGTTTGCCTGGCCGGGGATTCTGTTAGTCACCATTTTCGTGACTTGCCCTTTTGTTGCCCGA
GAATTAATCCCATTGATGCAGCAACAGGGCGCATCGGAGGAAGAAGCCGCGGTTATTCTTGGCGCCTCATGGTGGCAACT
GTTTCGCCGCGTGACTCTGCCCAATATCAAGTGGGCGCTGATTTATGGGGTGATCCTCACCAATGCCAGAGCCGTGGGTG
AGTTTGGTGCCGTTGCCGTAGTATCTGGCAATATCCGCGGTGAAACCAACACATTACCTTTGCATGTGCAATTACTTTAC
GAAGATTATCAGGCCGAAGCCGCCTTCGCGAGCGCGTCACTGCTTGCCTTGATCGCCCTATGTACCTTACTGCTAAAAGC
CTTAGTAGAGTGGCGCCAACAACGCAGCTTATCCGCCAATGACAACCAAGAGCAGAGCCAATCATTATGA

Upstream 100 bases:

>100_bases
TGCCTCCGTTGTACTGCTAACCTCGCTGTTGCTGCTACTGCTGATTAACCTGTGGCAGGCGCGTTATTTACGCCGCATTC
ACGGCCGATAAGGAGCCACC

Downstream 100 bases:

>100_bases
GTATTCGTTTAACTAATATTTCCAAAAAATTTGGCCAATTTCAGGCGCTCTCGCCGCTCAATCTGGATATTCAAGAAGGC
GAGATGATAGGTCTTCTCGG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MNSFKPLRVGEAPLIKWSLITLAVFLAMVLLLLPLVSIFQQAFVGGWERYIEHLSQPDSLHAIGLTLMVAALTVPINLVF
GVLLAWSVTRFEFPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGAWLFEHDLQIMFAWPGILLVTIFVTCPFVAR
ELIPLMQQQGASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALCTLLLKALVEWRQQRSLSANDNQEQSQSL

Sequences:

>Translated_289_residues
MNSFKPLRVGEAPLIKWSLITLAVFLAMVLLLLPLVSIFQQAFVGGWERYIEHLSQPDSLHAIGLTLMVAALTVPINLVF
GVLLAWSVTRFEFPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGAWLFEHDLQIMFAWPGILLVTIFVTCPFVAR
ELIPLMQQQGASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALCTLLLKALVEWRQQRSLSANDNQEQSQSL
>Mature_289_residues
MNSFKPLRVGEAPLIKWSLITLAVFLAMVLLLLPLVSIFQQAFVGGWERYIEHLSQPDSLHAIGLTLMVAALTVPINLVF
GVLLAWSVTRFEFPGRKLLITLIDIPFAVSPVVAGLLYLLLYGNSGWLGAWLFEHDLQIMFAWPGILLVTIFVTCPFVAR
ELIPLMQQQGASEEEAAVILGASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
EDYQAEAAFASASLLALIALCTLLLKALVEWRQQRSLSANDNQEQSQSL

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=272, Percent_Identity=60.2941176470588, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=41.5458937198068, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786980, Length=220, Percent_Identity=32.7272727272727, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31997; Mature: 31997

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFKPLRVGEAPLIKWSLITLAVFLAMVLLLLPLVSIFQQAFVGGWERYIEHLSQPDSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
HAIGLTLMVAALTVPINLVFGVLLAWSVTRFEFPGRKLLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCHHHHHHHHHHHHHH
LYGNSGWLGAWLFEHDLQIMFAWPGILLVTIFVTCPFVARELIPLMQQQGASEEEAAVIL
HHCCCCCEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEEEEEH
EDYQAEAAFASASLLALIALCTLLLKALVEWRQQRSLSANDNQEQSQSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNSFKPLRVGEAPLIKWSLITLAVFLAMVLLLLPLVSIFQQAFVGGWERYIEHLSQPDSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
HAIGLTLMVAALTVPINLVFGVLLAWSVTRFEFPGRKLLITLIDIPFAVSPVVAGLLYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCHHHHHHHHHHHHHH
LYGNSGWLGAWLFEHDLQIMFAWPGILLVTIFVTCPFVARELIPLMQQQGASEEEAAVIL
HHCCCCCEEHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
GASWWQLFRRVTLPNIKWALIYGVILTNARAVGEFGAVAVVSGNIRGETNTLPLHVQLLY
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCEEEEEEEH
EDYQAEAAFASASLLALIALCTLLLKALVEWRQQRSLSANDNQEQSQSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]