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The map label for this gene is cysU [H]

Identifier: 117921628

GI number: 117921628

Start: 3828266

End: 3829111

Strand: Direct

Name: cysU [H]

Synonym: Shewana3_3190

Alternate gene names: 117921628

Gene position: 3828266-3829111 (Clockwise)

Preceding gene: 117921627

Following gene: 117921629

Centisome position: 76.99

GC content: 53.55

Gene sequence:

>846_bases
GTGATATTTAATAATGGGCGCTTACGCCACAAGCGGGTTTTGCCGGGGTTTAGTATCAGCCTAGGTGTGTCTCTGCTGTT
TGTCAGCTTGATCCTCCTGCTCCCCACCACAGGCCTGATTATGCAAACCAGCCAGATGAGCTGGGCCGAGTATTGGGGCG
TGATTGCCGATCCCCGCGTGCTGGCAAGTTATAAAGTCACTATTTTATCTGCACTGGCCGCCTCCCTGTTTAACTGTCTT
TTTGGCCTGTTACTCGCCTGGGTGTTGGTTCGTTATGAGTTTCCGGGTAAGCGCATCCTCGATGCCTTAGTCGATTTACC
CTTTGCCTTACCCACAGCCGTCGCGGGTATCACCTTAGCGACCCTGTACGCCGAGAATGGCCAGATTGGTAGCCTGTTGG
CCGAAATCGGGGTCAAAGTGGCTTACACACCATTGGGGATCGTGGTTGCGATGATCTTCACCAGCATTCCCTTCGTGGTG
CGAACCGTCCAGCCCGTACTGGAAGAGTTATCCCACGATGAAGAAGAAGCTGGCATGACCTTAGGCGCAACCGATGGCGC
CGTGTTTTGGCGGGTGATTTTACCTTCCCTCTGGCCTGCCTTAGTGGTCGGTACGGCGCTGTCTTTTACCCGCAGCCTAG
GTGAGTTTGGCGCGGTGATTTTTATCGCAGGCAATATGCCCTACATCAGTGAAATCACCTCCCTGATGATTTTCGTTCGT
CTACAGGAATTTGATTTTGCCGGCGCCAGCGCCATTGCCTCCGTTGTACTGCTAACCTCGCTGTTGCTGCTACTGCTGAT
TAACCTGTGGCAGGCGCGTTATTTACGCCGCATTCACGGCCGATAA

Upstream 100 bases:

>100_bases
GGTCGCTATCACAAGTTGTCTTTATCGCGATTGAGCAATAATGTGCTAATGGCATAATAATATTGGATTAACTCCATTAA
CCTTATCGGGTGGTTATTCA

Downstream 100 bases:

>100_bases
GGAGCCACCATGAACTCATTTAAACCGTTAAGAGTCGGTGAAGCGCCCCTTATCAAATGGAGCCTAATCACCCTAGCCGT
GTTTTTGGCCATGGTATTGT

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MIFNNGRLRHKRVLPGFSISLGVSLLFVSLILLLPTTGLIMQTSQMSWAEYWGVIADPRVLASYKVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSLLAEIGVKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEELSHDEEEAGMTLGATDGAVFWRVILPSLWPALVVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLLTSLLLLLLINLWQARYLRRIHGR

Sequences:

>Translated_281_residues
MIFNNGRLRHKRVLPGFSISLGVSLLFVSLILLLPTTGLIMQTSQMSWAEYWGVIADPRVLASYKVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSLLAEIGVKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEELSHDEEEAGMTLGATDGAVFWRVILPSLWPALVVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLLTSLLLLLLINLWQARYLRRIHGR
>Mature_281_residues
MIFNNGRLRHKRVLPGFSISLGVSLLFVSLILLLPTTGLIMQTSQMSWAEYWGVIADPRVLASYKVTILSALAASLFNCL
FGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLATLYAENGQIGSLLAEIGVKVAYTPLGIVVAMIFTSIPFVV
RTVQPVLEELSHDEEEAGMTLGATDGAVFWRVILPSLWPALVVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
LQEFDFAGASAIASVVLLTSLLLLLLINLWQARYLRRIHGR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=272, Percent_Identity=67.2794117647059, Blast_Score=324, Evalue=3e-90,
Organism=Escherichia coli, GI87082099, Length=255, Percent_Identity=34.1176470588235, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=36.2068965517241, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30670; Mature: 30670

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNNGRLRHKRVLPGFSISLGVSLLFVSLILLLPTTGLIMQTSQMSWAEYWGVIADPRV
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCHH
LASYKVTILSALAASLFNCLFGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHH
TLYAENGQIGSLLAEIGVKVAYTPLGIVVAMIFTSIPFVVRTVQPVLEELSHDEEEAGMT
HHHHCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
LGATDGAVFWRVILPSLWPALVVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
LQEFDFAGASAIASVVLLTSLLLLLLINLWQARYLRRIHGR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFNNGRLRHKRVLPGFSISLGVSLLFVSLILLLPTTGLIMQTSQMSWAEYWGVIADPRV
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCHH
LASYKVTILSALAASLFNCLFGLLLAWVLVRYEFPGKRILDALVDLPFALPTAVAGITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHH
TLYAENGQIGSLLAEIGVKVAYTPLGIVVAMIFTSIPFVVRTVQPVLEELSHDEEEAGMT
HHHHCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
LGATDGAVFWRVILPSLWPALVVGTALSFTRSLGEFGAVIFIAGNMPYISEITSLMIFVR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
LQEFDFAGASAIASVVLLTSLLLLLLINLWQARYLRRIHGR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]