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The map label for this gene is oprF [H]

Identifier: 117921584

GI number: 117921584

Start: 3773981

End: 3775102

Strand: Direct

Name: oprF [H]

Synonym: Shewana3_3146

Alternate gene names: 117921584

Gene position: 3773981-3775102 (Clockwise)

Preceding gene: 117921583

Following gene: 117921585

Centisome position: 75.9

GC content: 44.83

Gene sequence:

>1122_bases
ATGGACTTAGAGAAAATGATGAAGAATACATTAAAAGTAGTTTTGCTTACATCAATGCTTCCACTTGCTGCCAGTGCATC
TCAAGAGCTAACACCTTGGTATGTTGGTGCAGGTTTAGGCGTTAATAACTATGAACACATTGCAACTGATAACGGCGATG
ACAACCCATACGCATGGGACATCTTCGCTGGCTATATGTTTAATGACTACTTTGGTGCAGAAATCGGCTATCGTGATCTA
GGTAGTGCAGATTGGACCTATGCAGGCATTGGCAATGATGCAGATGTAAAAGGTGCAACTCTTGGCTTAGTGGGCGTGTG
GCCACTAGGTAACCGTTGGAGCCTGTCTGCTGAAGCTGGCGCAATGTACTACACCCTAGAAAACAACCAACGCGTTGGTG
GCGTATCTAGTTCTTACAGCGAAAACGACTTCGCACCTTACTTCGGTGCAGGTGTTGGCTATAACTTCACTGATAACCTG
AAGTTACAAGCTAAATACCGTCGCTATGAAAACTTAGATGATAACGCTGGCGCTAACGCTATCGTTCCAGTAAATGCAGA
CAGCAACTACTGGGGCTTAGAATTAAGCTACCGTTTTGGTACTGCCGCGGCAGCACCAGTGGCAGCAGCCGTTGTTGCAG
CAACTCCAGTTGATTCTGACAACGATGGTGTTTACGACGATAAAGATCAGTGCCCAGCAACTCCAGCAACTCACAAAGTT
GACTCTGTTGGCTGTACTATCTACGAAAACGTTAAGAAGCAAGAAGATGTAGGTTCTATCCAGTTTGCTAACGATTCTGC
TGTAGTGAAGAAAGAGTACTACAAAGACATCGAAAGACTGGCTAATTACATGAACAAGAACCCAGAATTCACTGTTGAAA
TTGCGGGTCACGCTTCTAACGTAGGTAAACCAGAATACAACATGGTTCTGTCTGACAAGCGTGCTGACGCTGTTGCTAAG
ATCCTTGTTGAAAAATATGGCATCAGCCAAAGCCGTGTAACTTCTAACGGTTACGGTATCACTAAGCCATTAGTGGCTGG
TGACTCTAAAGAAGCTCATGCAGCTAACCGTCGCATCGAAGCTATCGTGACAACTACTGAAAAACAACCTGTTCTGAAGT
AA

Upstream 100 bases:

>100_bases
ATAATTAATCGCTGGCTACAATACCAAAGGATCCCCACACTAAGTGCTAGCAATGTTTTGCATAAAACCTGCAAAGCATA
TTGGTGGAGAATCATAAACA

Downstream 100 bases:

>100_bases
TTGCACTCTTAAGAATAAAAAAGTCGCTGCTTGCAGCGACTTTTTATTTTTATAACATCTACACTATGCAATCTGATGAT
GCTTAGCTAATCGTTGTAAT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MDLEKMMKNTLKVVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGDDNPYAWDIFAGYMFNDYFGAEIGYRDL
GSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAGAMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNL
KLQAKYRRYENLDDNAGANAIVPVNADSNYWGLELSYRFGTAAAAPVAAAVVAATPVDSDNDGVYDDKDQCPATPATHKV
DSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYMNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAK
ILVEKYGISQSRVTSNGYGITKPLVAGDSKEAHAANRRIEAIVTTTEKQPVLK

Sequences:

>Translated_373_residues
MDLEKMMKNTLKVVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGDDNPYAWDIFAGYMFNDYFGAEIGYRDL
GSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAGAMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNL
KLQAKYRRYENLDDNAGANAIVPVNADSNYWGLELSYRFGTAAAAPVAAAVVAATPVDSDNDGVYDDKDQCPATPATHKV
DSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYMNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAK
ILVEKYGISQSRVTSNGYGITKPLVAGDSKEAHAANRRIEAIVTTTEKQPVLK
>Mature_373_residues
MDLEKMMKNTLKVVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGDDNPYAWDIFAGYMFNDYFGAEIGYRDL
GSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAGAMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNL
KLQAKYRRYENLDDNAGANAIVPVNADSNYWGLELSYRFGTAAAAPVAAAVVAATPVDSDNDGVYDDKDQCPATPATHKV
DSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYMNKNPEFTVEIAGHASNVGKPEYNMVLSDKRADAVAK
ILVEKYGISQSRVTSNGYGITKPLVAGDSKEAHAANRRIEAIVTTTEKQPVLK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787191, Length=370, Percent_Identity=23.2432432432432, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 40507; Mature: 40507

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLEKMMKNTLKVVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGDDNPYAWD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCEEEE
IFAGYMFNDYFGAEIGYRDLGSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAG
EEECHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCC
AMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNLKLQAKYRRYENLDDNAGANA
EEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCE
IVPVNADSNYWGLELSYRFGTAAAAPVAAAVVAATPVDSDNDGVYDDKDQCPATPATHKV
EEEECCCCCEEEEEEEEECCCCHHHHHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCCC
DSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYMNKNPEFTVEIAGHASN
CCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
VGKPEYNMVLSDKRADAVAKILVEKYGISQSRVTSNGYGITKPLVAGDSKEAHAANRRIE
CCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCEECCCCCHHHHHCCEEE
AIVTTTEKQPVLK
EEEEECCCCCCCC
>Mature Secondary Structure
MDLEKMMKNTLKVVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGDDNPYAWD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCEEEE
IFAGYMFNDYFGAEIGYRDLGSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAG
EEECHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCC
AMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNLKLQAKYRRYENLDDNAGANA
EEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCE
IVPVNADSNYWGLELSYRFGTAAAAPVAAAVVAATPVDSDNDGVYDDKDQCPATPATHKV
EEEECCCCCEEEEEEEEECCCCHHHHHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCCC
DSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYMNKNPEFTVEIAGHASN
CCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
VGKPEYNMVLSDKRADAVAKILVEKYGISQSRVTSNGYGITKPLVAGDSKEAHAANRRIE
CCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCEECCCCCHHHHHCCEEE
AIVTTTEKQPVLK
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]