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The map label for this gene is sseA [H]

Identifier: 117921541

GI number: 117921541

Start: 3722010

End: 3722867

Strand: Reverse

Name: sseA [H]

Synonym: Shewana3_3103

Alternate gene names: 117921541

Gene position: 3722867-3722010 (Counterclockwise)

Preceding gene: 117921542

Following gene: 117921540

Centisome position: 74.87

GC content: 50.7

Gene sequence:

>858_bases
ATGGAATATCCTCTCGTGAGTACTCAATGGCTCGAAGAGCATCTCACCTCGCCAGATCTCGTGTTACTCGATGCCAGCAT
GGCCGTGGTGTTGGGCAAAGAGCCTATTCTCTATTCCGAACCTGTGTGCATTCCGCGCTCGAGGCGCTTTGATGTCGAAA
CGGATTTTTGCGATAAAACGTCTTCTCAAATCCACGCCTTACCAAGGTTTGAACAGTTTATAGAAGGTGTTTCCAAGCTC
GGAATAGACCCGCAAAGCGTGGTGGTTATCTATGATAATCAAGGGATTTATTCTTCTCCGCGCGCTTGGTGGATATTCAA
GGTGATGGGATTTCACCGTGTGTATGTGCTCGACGGTGGCTTACCCCAATGGATGGCTGAAGACAGGGTCACCTCATCCC
GCTATCAGGAAGAAGGGATAGATTATGGTGCAAGCGATAGTGAAGAACTCACTGCAGTACTGGAATATCAGCCTGCCAAG
GTGATGGATGCCGAGGCCGTGTTCGCCAAGCTAGATGATAGCGACAGCGCGATTATCGATGCCCGCGGCGCCGCGCGCTT
TTTAGGTCAAGTGAGTGAGCCACGCCCAGGTGTGCGTAGTGGACATATTCCCCATTCGGTTAATTTACCTTTTACAGAGG
TGCTCGATGGCTTCAAAATCAAAAGTTCGGCCGAATTACAAACCCTGTTTCAAGGGCTTGCTGGCGATAAACCGTTAAGG
ATTTTCAGCTGTGGCTCGGGCATTACCGCCTGTATCTTAATCTTAGCGTCGATCGCTTCTGGTCATGCCGATGCTGTGCT
TTACGATGGCTCGTGGGCCGACTGGGGCAGTCGCAGCGATTTACCCATAGCGCAATAA

Upstream 100 bases:

>100_bases
CGGTTCGAATGGTTTGAATAGTGCTTGGGTTTTAGTATGTGCAGTAAAACTCAGGCTGATAAGTCATTATCCCAGTTGGA
AATCAGAATAAGGACAGCCA

Downstream 100 bases:

>100_bases
GCGCTGATAGCAGGAGTGCTGTGCAGGATAACCAGCAGCACTCCATTGGCAGTGATATATCCTTTGACTTATCAAGGAGT
TTGCCATGATAACTCGATTG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEYPLVSTQWLEEHLTSPDLVLLDASMAVVLGKEPILYSEPVCIPRSRRFDVETDFCDKTSSQIHALPRFEQFIEGVSKL
GIDPQSVVVIYDNQGIYSSPRAWWIFKVMGFHRVYVLDGGLPQWMAEDRVTSSRYQEEGIDYGASDSEELTAVLEYQPAK
VMDAEAVFAKLDDSDSAIIDARGAARFLGQVSEPRPGVRSGHIPHSVNLPFTEVLDGFKIKSSAELQTLFQGLAGDKPLR
IFSCGSGITACILILASIASGHADAVLYDGSWADWGSRSDLPIAQ

Sequences:

>Translated_285_residues
MEYPLVSTQWLEEHLTSPDLVLLDASMAVVLGKEPILYSEPVCIPRSRRFDVETDFCDKTSSQIHALPRFEQFIEGVSKL
GIDPQSVVVIYDNQGIYSSPRAWWIFKVMGFHRVYVLDGGLPQWMAEDRVTSSRYQEEGIDYGASDSEELTAVLEYQPAK
VMDAEAVFAKLDDSDSAIIDARGAARFLGQVSEPRPGVRSGHIPHSVNLPFTEVLDGFKIKSSAELQTLFQGLAGDKPLR
IFSCGSGITACILILASIASGHADAVLYDGSWADWGSRSDLPIAQ
>Mature_285_residues
MEYPLVSTQWLEEHLTSPDLVLLDASMAVVLGKEPILYSEPVCIPRSRRFDVETDFCDKTSSQIHALPRFEQFIEGVSKL
GIDPQSVVVIYDNQGIYSSPRAWWIFKVMGFHRVYVLDGGLPQWMAEDRVTSSRYQEEGIDYGASDSEELTAVLEYQPAK
VMDAEAVFAKLDDSDSAIIDARGAARFLGQVSEPRPGVRSGHIPHSVNLPFTEVLDGFKIKSSAELQTLFQGLAGDKPLR
IFSCGSGITACILILASIASGHADAVLYDGSWADWGSRSDLPIAQ

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=287, Percent_Identity=39.0243902439024, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI61835204, Length=287, Percent_Identity=39.0243902439024, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI194473681, Length=287, Percent_Identity=39.0243902439024, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI17402865, Length=290, Percent_Identity=36.2068965517241, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI87082121, Length=283, Percent_Identity=36.0424028268551, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17561888, Length=264, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71997283, Length=311, Percent_Identity=23.4726688102894, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=251, Percent_Identity=31.8725099601594, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31390; Mature: 31390

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYPLVSTQWLEEHLTSPDLVLLDASMAVVLGKEPILYSEPVCIPRSRRFDVETDFCDKT
CCCCCCCHHHHHHHCCCCCEEEEECCEEEEECCCCCEECCCCCCCCCCCCCCCCCHHCCC
SSQIHALPRFEQFIEGVSKLGIDPQSVVVIYDNQGIYSSPRAWWIFKVMGFHRVYVLDGG
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEEEEEECC
LPQWMAEDRVTSSRYQEEGIDYGASDSEELTAVLEYQPAKVMDAEAVFAKLDDSDSAIID
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCHHCCHHHHHEECCCCCCEEEE
ARGAARFLGQVSEPRPGVRSGHIPHSVNLPFTEVLDGFKIKSSAELQTLFQGLAGDKPLR
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
IFSCGSGITACILILASIASGHADAVLYDGSWADWGSRSDLPIAQ
EEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEYPLVSTQWLEEHLTSPDLVLLDASMAVVLGKEPILYSEPVCIPRSRRFDVETDFCDKT
CCCCCCCHHHHHHHCCCCCEEEEECCEEEEECCCCCEECCCCCCCCCCCCCCCCCHHCCC
SSQIHALPRFEQFIEGVSKLGIDPQSVVVIYDNQGIYSSPRAWWIFKVMGFHRVYVLDGG
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEEEEEECC
LPQWMAEDRVTSSRYQEEGIDYGASDSEELTAVLEYQPAKVMDAEAVFAKLDDSDSAIID
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCHHCCHHHHHEECCCCCCEEEE
ARGAARFLGQVSEPRPGVRSGHIPHSVNLPFTEVLDGFKIKSSAELQTLFQGLAGDKPLR
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
IFSCGSGITACILILASIASGHADAVLYDGSWADWGSRSDLPIAQ
EEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]