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The map label for this gene is potF [H]

Identifier: 117921535

GI number: 117921535

Start: 3715037

End: 3716200

Strand: Reverse

Name: potF [H]

Synonym: Shewana3_3097

Alternate gene names: 117921535

Gene position: 3716200-3715037 (Counterclockwise)

Preceding gene: 117921536

Following gene: 117921534

Centisome position: 74.74

GC content: 45.36

Gene sequence:

>1164_bases
ATGGTCGGTGACACAATTTCAGAATTGTTAAAGGCAATTCTGCTACCCGAGAAAGGAGAAAGCATGAAGCTATTTAATAA
GATGACGACTTTGGCTTTAGTAACCGCGGGCGCCCTCGCAAGCGTTGCTGCCCAAGCTGAAGAAGTAGTGCGCGTATATA
ACTGGTCTGATTATATCGCGGAAGATACCTTAGAAAACTTCAAGAAAGAAACGGGCATTCGGGTGATTTACGATGTGTTT
GATAGTAACGAAGTGCTGGAAGCCAAGTTACTGTCTGGCCGCAGTGGTTACGACATCGTGGTTCCATCTAACCATTTCCT
CGCTAAACAAATTAAGGCTGGCGCATTCAAGCCGCTAGACCGCTCTAAACTCAGCAACTTTAAAAACCTCAACGCCGATC
TAATGAAACAGTTAGAGAAGGCTGATCCGGGTAACCAATATGCCGTACCTTACCTCTGGGGTACCAATGGTATCGGTTAC
AACATCGATAAAGTGAAAGCCGCAGTGGGTGAAGATGCGCCATTTGATTCTATGGAGCTGATCTTTAACCCAAAATATGC
TGAAAAAATCTCTAAGTGTGGCTTTGCTATGCTGGATTCGGCCGATGATATGGTGCCACAGGCGATGATCTACTTAGGTC
TTGACCCAAATAGCACGAAAGCCGAAGACTATGAAAAAGCAGGTGAATTACTCGAAAAAATTCGCCCTTACGTGACTTAT
TTCCATTCATCACGCTATATCTCTGATCTCGCGAATGGCGATATCTGTGTGGCATTTGGCTTCTCGGGTGACGTATTCCA
AGCTGCGGCCCGTGCCGATGAAGCGGGTAACGGTAACAAGATTGGTTACTCGATTCCAAAAGAAGGCGCGAACCTGTGGT
TCGATATGTTAGCTATCCCTGCGGATGCGGCTAACGTTGAAAATGCTCACAAACTTATCAACTACTTACTTCGTCCTGAA
GTGATTGCGCCTATCTCTAACTATGTGGCTTATGCTAACCCTAACGACCCAGCACAGCCTTTAGTTGATGAAGCGATTCG
CAATGACCCTGCGATTTATCCACCAAAAGAAGTGTTAGATAAATTGTATATTGGCGAAATCCGTCCATTGAAAATCCAAC
GTGTATTAACCCGCGTCTGGACCAAAGTGAAGTCTGGCCAATAA

Upstream 100 bases:

>100_bases
TGCAGCCACCTAGAGATGAAGGCATTATGGTGCTGTAGAGACTGCCGATGCGGTTTTTAAGGTGTAGCATTGGTCGAGAA
TAACAACAATAACCGAGCAG

Downstream 100 bases:

>100_bases
TCGAGATGGGGCAAGCATTGCTTGCCCCTTTTAATAACGCAGACATTGGCTTGCTCCTTGAGCATTCGGCCTACAGATTT
TATCTCTATTCATTAATTCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MVGDTISELLKAILLPEKGESMKLFNKMTTLALVTAGALASVAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVF
DSNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRSKLSNFKNLNADLMKQLEKADPGNQYAVPYLWGTNGIGY
NIDKVKAAVGEDAPFDSMELIFNPKYAEKISKCGFAMLDSADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTY
FHSSRYISDLANGDICVAFGFSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIPADAANVENAHKLINYLLRPE
VIAPISNYVAYANPNDPAQPLVDEAIRNDPAIYPPKEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Sequences:

>Translated_387_residues
MVGDTISELLKAILLPEKGESMKLFNKMTTLALVTAGALASVAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVF
DSNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRSKLSNFKNLNADLMKQLEKADPGNQYAVPYLWGTNGIGY
NIDKVKAAVGEDAPFDSMELIFNPKYAEKISKCGFAMLDSADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTY
FHSSRYISDLANGDICVAFGFSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIPADAANVENAHKLINYLLRPE
VIAPISNYVAYANPNDPAQPLVDEAIRNDPAIYPPKEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ
>Mature_387_residues
MVGDTISELLKAILLPEKGESMKLFNKMTTLALVTAGALASVAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVF
DSNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRSKLSNFKNLNADLMKQLEKADPGNQYAVPYLWGTNGIGY
NIDKVKAAVGEDAPFDSMELIFNPKYAEKISKCGFAMLDSADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTY
FHSSRYISDLANGDICVAFGFSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIPADAANVENAHKLINYLLRPE
VIAPISNYVAYANPNDPAQPLVDEAIRNDPAIYPPKEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=371, Percent_Identity=54.9865229110512, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=34.5132743362832, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787711, Length=317, Percent_Identity=23.3438485804416, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42739; Mature: 42739

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGDTISELLKAILLPEKGESMKLFNKMTTLALVTAGALASVAAQAEEVVRVYNWSDYIA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLD
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCC
RSKLSNFKNLNADLMKQLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFDSMEL
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHE
IFNPKYAEKISKCGFAMLDSADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTY
EECCHHHHHHHHCCHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
FHSSRYISDLANGDICVAFGFSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIP
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCC
ADAANVENAHKLINYLLRPEVIAPISNYVAYANPNDPAQPLVDEAIRNDPAIYPPKEVLD
CCCCCHHHHHHHHHHHHCCHHHHHHHHCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHH
KLYIGEIRPLKIQRVLTRVWTKVKSGQ
HHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVGDTISELLKAILLPEKGESMKLFNKMTTLALVTAGALASVAAQAEEVVRVYNWSDYIA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLD
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCC
RSKLSNFKNLNADLMKQLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFDSMEL
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHE
IFNPKYAEKISKCGFAMLDSADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTY
EECCHHHHHHHHCCHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
FHSSRYISDLANGDICVAFGFSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIP
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCC
ADAANVENAHKLINYLLRPEVIAPISNYVAYANPNDPAQPLVDEAIRNDPAIYPPKEVLD
CCCCCHHHHHHHHHHHHCCHHHHHHHHCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHH
KLYIGEIRPLKIQRVLTRVWTKVKSGQ
HHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]