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The map label for this gene is gltD [H]

Identifier: 117921489

GI number: 117921489

Start: 3658672

End: 3660078

Strand: Direct

Name: gltD [H]

Synonym: Shewana3_3051

Alternate gene names: 117921489

Gene position: 3658672-3660078 (Clockwise)

Preceding gene: 117921488

Following gene: 117921490

Centisome position: 73.58

GC content: 53.94

Gene sequence:

>1407_bases
ATGAGTAACGATTTTCAATTTATTGAAGTGGGTCGCAAAGACCCAACTAAACACCCTGCAGCAAAGCGTTCGACTCAGTT
TATTGAGATTTATCAGCCCTTTGCTCAAGCTCAGGTGACTGAACAGGCGGACCGTTGTCTCGATTGCGGTAACCCTTATT
GTGAGTGGAAATGCCCGCTGCACAACTATATTCCCAACTGGCTGAAACTGGCGCAGCAGGGGCGAATTATGGAAGCGGCG
GATTTGGTCCATGAGACTAATACCCTGCCCGAGATCTGCGGCCGCGTCTGCCCACAGGACAGACTCTGTGAAGGTGCTTG
TACCCTAAACGCCGACTTTGGTGCCGTGACTATCGGTAATGTGGAGAAGTACATTACCGATACCGCCATCGCCCAAGGCT
GGCGTCCTGATATGAGCAAGGTCACGCCACGCAAGGAAAAGGTCGCGATTGTGGGCGCTGGCCCTGCTGGTTTAGGCTGT
GCCGATATTCTTGCCCGTAATGGGATTAAGGCTGTGGTGTTTGATAAGTATCCCCAGATTGGTGGCCTGCTGACCTACGG
TATTCCGGCTTTTAAACTGGATAAAGCCGTGATGGCGACTCGCCGCACTGTGTTAGAAGGCATGGGCATCGAGTTCAAGC
TGGGCGTCACTGTCGGTAAAGACGTCGCCTTTAACCAACTGCTAGAGGAATACGATGCAGTCTTCCTCGGCATGGGAACC
TACACCGCCATGAAAGCGGGCCTTGAGGGCGAAGATGCCCAAGGCGTGTACCAAGCCCTGCCCTACTTGATTGGCAATAC
CCATCACCTGATGGGCAGCAGTAATCCTGACACCCCTTACCTGAATCTTGCCGGTAAGCGCGTGGTGGTCTTAGGTGGCG
GTGATACCGCCATGGACTGTGTCCGCACCGCCGTGCGCCAAGGGGCCAGCAGTGTAATTTGTGCCTATCGCCGTGACGAA
GCCAACATGCCTGGTTCACGCCGCGAGGTGCAAAACGCCCGTGAAGAGGGCGTGAACTTCCTGTTTAACCGTCAACCCGT
GGCGATTAAAACCCAGGATGGCAAAGTAGTGGGCGTGGAATGCGTTGAAACCCAAATGGGTAAAGCCGATGCCAGCGGCC
GTCAACGTGCCGAAGCGATTGAAGGCAGCGAGCAGTTACTCGACGCCGATGCGGTGATTATCGCCTTCGGTTTCCAACCT
AGCCCTGCGCCTTGGTTTGCCGATTACGGCATTGAACTAGACCAATGGGGCCGAGTAAAAGCCAGTAAACAGGCCGATAA
CCCATTCCAAACCACCAATCCAAAGGTATTTGCTGGCGGTGATATGGTCCGAGGTTCGGATCTGGTTGTGACCGCGATTG
CCGAAGGACGTGATGCCGCACAAGGTATTCTTAACTACTTAGATTAA

Upstream 100 bases:

>100_bases
TTGCTTTGTTTTAGTGAAACCGAAAAATATCGCCGTTGCAGATCTGCTCAAATTAGAGCAATCGAGCCCGGAACTAGCAG
TTAAAGCGGGGTAATAGCAG

Downstream 100 bases:

>100_bases
TTGTTTTTTGTTGTACCGCTGTACCTGTGAGTCTCGGACTCGTACCCACGAGTCCACCATTTGCTTGTGAATTTTGGCGC
ACCTTAGGGTGCGCATTTTT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSNDFQFIEVGRKDPTKHPAAKRSTQFIEIYQPFAQAQVTEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAQQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNADFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRKEKVAIVGAGPAGLGC
ADILARNGIKAVVFDKYPQIGGLLTYGIPAFKLDKAVMATRRTVLEGMGIEFKLGVTVGKDVAFNQLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGSSNPDTPYLNLAGKRVVVLGGGDTAMDCVRTAVRQGASSVICAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGVECVETQMGKADASGRQRAEAIEGSEQLLDADAVIIAFGFQP
SPAPWFADYGIELDQWGRVKASKQADNPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Sequences:

>Translated_468_residues
MSNDFQFIEVGRKDPTKHPAAKRSTQFIEIYQPFAQAQVTEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAQQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNADFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRKEKVAIVGAGPAGLGC
ADILARNGIKAVVFDKYPQIGGLLTYGIPAFKLDKAVMATRRTVLEGMGIEFKLGVTVGKDVAFNQLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGSSNPDTPYLNLAGKRVVVLGGGDTAMDCVRTAVRQGASSVICAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGVECVETQMGKADASGRQRAEAIEGSEQLLDADAVIIAFGFQP
SPAPWFADYGIELDQWGRVKASKQADNPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
>Mature_467_residues
SNDFQFIEVGRKDPTKHPAAKRSTQFIEIYQPFAQAQVTEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAQQGRIMEAAD
LVHETNTLPEICGRVCPQDRLCEGACTLNADFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRKEKVAIVGAGPAGLGCA
DILARNGIKAVVFDKYPQIGGLLTYGIPAFKLDKAVMATRRTVLEGMGIEFKLGVTVGKDVAFNQLLEEYDAVFLGMGTY
TAMKAGLEGEDAQGVYQALPYLIGNTHHLMGSSNPDTPYLNLAGKRVVVLGGGDTAMDCVRTAVRQGASSVICAYRRDEA
NMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGVECVETQMGKADASGRQRAEAIEGSEQLLDADAVIIAFGFQPS
PAPWFADYGIELDQWGRVKASKQADNPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=30.3097345132743, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=66.4529914529915, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=59.7826086956522, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=56.304347826087, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=29.2792792792793, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=29.8823529411765, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=32.9938900203666, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=471, Percent_Identity=30.7855626326964, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17543860, Length=207, Percent_Identity=31.8840579710145, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=482, Percent_Identity=31.7427385892116, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=36.9072164948454, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=36.9072164948454, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=36.9072164948454, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=36.9072164948454, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24640763, Length=474, Percent_Identity=28.4810126582279, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=474, Percent_Identity=28.4810126582279, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50800; Mature: 50669

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFIEVGRKDPTKHPAAKRSTQFIEIYQPFAQAQVTEQADRCLDCGNPYCEWKCPL
CCCCCEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAQQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNADFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHCCCEEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRKEKVAIVGAGPAGLGCADILARNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCC
GGLLTYGIPAFKLDKAVMATRRTVLEGMGIEFKLGVTVGKDVAFNQLLEEYDAVFLGMGT
CCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGSSNPDTPYLNLAGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVICAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGVE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEE
CVETQMGKADASGRQRAEAIEGSEQLLDADAVIIAFGFQPSPAPWFADYGIELDQWGRVK
EHHHHCCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCC
ASKQADNPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNDFQFIEVGRKDPTKHPAAKRSTQFIEIYQPFAQAQVTEQADRCLDCGNPYCEWKCPL
CCCCEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAQQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNADFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHCCCEEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRKEKVAIVGAGPAGLGCADILARNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCC
GGLLTYGIPAFKLDKAVMATRRTVLEGMGIEFKLGVTVGKDVAFNQLLEEYDAVFLGMGT
CCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGSSNPDTPYLNLAGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVICAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVVGVE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEE
CVETQMGKADASGRQRAEAIEGSEQLLDADAVIIAFGFQPSPAPWFADYGIELDQWGRVK
EHHHHCCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCC
ASKQADNPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]