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The map label for this gene is grpE [H]

Identifier: 117921343

GI number: 117921343

Start: 3471986

End: 3472606

Strand: Reverse

Name: grpE [H]

Synonym: Shewana3_2902

Alternate gene names: 117921343

Gene position: 3472606-3471986 (Counterclockwise)

Preceding gene: 117921350

Following gene: 117921339

Centisome position: 69.84

GC content: 48.47

Gene sequence:

>621_bases
ATGAGCAACGAGTCGATTAAAGCAGAACAGGATCTGATCCAAGAGGGTGTAGAGTCTGAAGTTTCTACCGAAGAAGCGAG
TCTGATTGACGAGCTTACCCAAGCCAATTTCCGTATTGAAGAGCTGGAACAGTTACTCGCTGACGCTTTGGCGAAAGTTG
AAGAGCAAAAAGATTCAGTTATCCGTGCTGCTGCCGAAGTAGACAACATTCGCCGCCGTGCTGCGATGGATGTGGAAAAG
GCGAACAAGTTTGCATTGGAAAAATTCGCCAACGAATTACTGCCAGTGTTAGACAATATGGAACGTGCGCTGCAAGGTAC
TAATCCACAGGATGAAACCACCAAAGCACTGTTCGAAGGTGTTGAGCTTACGCAAAAGAGCTTCTTGACCGCCGTGGCTA
AGTTTGGGGTAAAACCTATCGATCCACAGGGGCAAGCCTTCAACCCTGACCAACATCAAGCCATTGGTATGCAGCCAAGC
GCTGAGTATCCAGCCAATACTGTGATGTTAGTGATGCAAAAGGGTTATGAGCTGAACAGCCGTTTACTGCGTCCTGCCAT
GGTGATGGTGTCACAGGGCGGCCCTAGCCAAGAATCTGCTTCAATCAACATTGAAGCCTGA

Upstream 100 bases:

>100_bases
TATTTATGCTAGATCTGGCCTTAGGCCTTGAAACGTCAAAATTGATCCCCATAATATGCCCAGAGTATTTAGAAACAAAG
CATTTTTAGCTGGAGTGAAA

Downstream 100 bases:

>100_bases
TTGAAGCCTAAGTTAACGCACTGAGCGTTAAAGGTTACGTAATAAAAAGGACTGCAGATGCAGTCCTTTTTATTTGTCTG
TTTTTTGCCTGCTAATCGCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQLLADALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALQGTNPQDETTKALFEGVELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPS
AEYPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPSQESASINIEA

Sequences:

>Translated_206_residues
MSNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQLLADALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALQGTNPQDETTKALFEGVELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPS
AEYPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPSQESASINIEA
>Mature_205_residues
SNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQLLADALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEKA
NKFALEKFANELLPVLDNMERALQGTNPQDETTKALFEGVELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPSA
EYPANTVMLVMQKGYELNSRLLRPAMVMVSQGGPSQESASINIEA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=158, Percent_Identity=33.5443037974684, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=143, Percent_Identity=32.1678321678322, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1788967, Length=179, Percent_Identity=43.5754189944134, Blast_Score=140, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653432, Length=165, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22680; Mature: 22549

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQLLADALAKVEEQKDSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALQGTNPQDETTKALFEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPSAEYPANTVMLVMQKGYELNS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCHHH
RLLRPAMVMVSQGGPSQESASINIEA
HHHHHHHHHHCCCCCCCCCCCEEECC
>Mature Secondary Structure 
SNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQLLADALAKVEEQKDSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALQGTNPQDETTKALFEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPSAEYPANTVMLVMQKGYELNS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCHHH
RLLRPAMVMVSQGGPSQESASINIEA
HHHHHHHHHHCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA