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The map label for this gene is truC [H]

Identifier: 117921265

GI number: 117921265

Start: 3373305

End: 3374159

Strand: Reverse

Name: truC [H]

Synonym: Shewana3_2824

Alternate gene names: 117921265

Gene position: 3374159-3373305 (Counterclockwise)

Preceding gene: 117921266

Following gene: 117921264

Centisome position: 67.86

GC content: 49.47

Gene sequence:

>855_bases
ATGAACCTAGATGATGATTGGCTGGATATGCCAGAGGATAAATTCAACCCAACCGAGAGCGACGAACAGTCAGAGCCTGC
GCCTGAACTGCGTGTTTTGTATCAAGACGAGCACTTGGTGGCTATCCATAAACCCGCGGGATTATTGGTTCATCGCAGCT
ACTTAGCACGGCGTGAACGCTTTTTTGCGATGCAACTGACTCGTGACTTGGTCGGTTGCCATGTGTTTCCGGTACACCGT
TTAGATAGACCGACCTCGGGCGTGTTGTTATTTGCCAAAAGCAGTGAGGTGGCCAATGCCCTATGTGAGCAATTTGCCAA
CCACAGTATTGAAAAACAATATTTGGCACTGGTGCGTGGCAATATGCACGAGAGTGGTGTGTTGGATTATGCGCTCAAAG
TCGAATTGGATGAGGTGGCTGACAAGTTTGCCAATCAAGATAAGGCCGCGCAGGACGCTGTGACTCAATATGAGCCGCTA
TTAAATACCGAAATTCCTTATCCGTCGGGGCGTTACGCTACCAGCCGTTTCGCGTTAGTGAAACTGAGTCCAAAAACGGG
GCGTAAGCATCAACTCAGACGCCATATGGCGCATTTACGTCACCCGATTATTGGCGATACCACGCACGGAGATGGTAAGC
AAAATGCGTTTTTCCGTACGCATTTCGATATCAATCGATTGTGGCTGATTGCGAAGAAGTTAACCTTCACTCACCCAGTT
ACCCAAGCGCGACTGAGTATCGAAACCGAGCTTGAGCCAGAATGGGAAACCGTGTTTTCAGGACTGGGCTGGGATGATGC
CGCCCTTTGTTGCGAGCCAAGTGTGTTGATTGCAGAGCAAACGACGACCGTTTAA

Upstream 100 bases:

>100_bases
AGTAAAATTGCGATAGCCCCGATGGCGGAACATGTGTGGTCCGAACAAGCGGCGTTAACACCTTTAATCGGTGTCTTAAA
TGAATTGGATATGTTGTTAA

Downstream 100 bases:

>100_bases
GGCAATGCTTGTTTGGGCATTTAGGGCCTGTGGTCGCTTAAGAGGCGACTACTAACTTAAAGATACTCCTAACCAAAGGA
GAGTGCTCACTTAAGCTTGC

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNLDDDWLDMPEDKFNPTESDEQSEPAPELRVLYQDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFANHSIEKQYLALVRGNMHESGVLDYALKVELDEVADKFANQDKAAQDAVTQYEPL
LNTEIPYPSGRYATSRFALVKLSPKTGRKHQLRRHMAHLRHPIIGDTTHGDGKQNAFFRTHFDINRLWLIAKKLTFTHPV
TQARLSIETELEPEWETVFSGLGWDDAALCCEPSVLIAEQTTTV

Sequences:

>Translated_284_residues
MNLDDDWLDMPEDKFNPTESDEQSEPAPELRVLYQDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFANHSIEKQYLALVRGNMHESGVLDYALKVELDEVADKFANQDKAAQDAVTQYEPL
LNTEIPYPSGRYATSRFALVKLSPKTGRKHQLRRHMAHLRHPIIGDTTHGDGKQNAFFRTHFDINRLWLIAKKLTFTHPV
TQARLSIETELEPEWETVFSGLGWDDAALCCEPSVLIAEQTTTV
>Mature_284_residues
MNLDDDWLDMPEDKFNPTESDEQSEPAPELRVLYQDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFANHSIEKQYLALVRGNMHESGVLDYALKVELDEVADKFANQDKAAQDAVTQYEPL
LNTEIPYPSGRYATSRFALVKLSPKTGRKHQLRRHMAHLRHPIIGDTTHGDGKQNAFFRTHFDINRLWLIAKKLTFTHPV
TQARLSIETELEPEWETVFSGLGWDDAALCCEPSVLIAEQTTTV

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=32.7956989247312, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI17158025, Length=150, Percent_Identity=31.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI23308689, Length=190, Percent_Identity=29.4736842105263, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI221316663, Length=204, Percent_Identity=29.9019607843137, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=55.7446808510638, Blast_Score=267, Evalue=6e-73,
Organism=Escherichia coli, GI1787327, Length=227, Percent_Identity=34.3612334801762, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786244, Length=230, Percent_Identity=33.4782608695652, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788946, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320168, Length=186, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321608, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324506, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583449, Length=177, Percent_Identity=28.2485875706215, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583447, Length=177, Percent_Identity=28.2485875706215, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583445, Length=177, Percent_Identity=28.2485875706215, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921090, Length=177, Percent_Identity=27.1186440677966, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 32371; Mature: 32371

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDDDWLDMPEDKFNPTESDEQSEPAPELRVLYQDEHLVAIHKPAGLLVHRSYLARRER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHH
FFAMQLTRDLVGCHVFPVHRLDRPTSGVLLFAKSSEVANALCEQFANHSIEKQYLALVRG
HHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHC
NMHESGVLDYALKVELDEVADKFANQDKAAQDAVTQYEPLLNTEIPYPSGRYATSRFALV
CCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
KLSPKTGRKHQLRRHMAHLRHPIIGDTTHGDGKQNAFFRTHFDINRLWLIAKKLTFTHPV
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHEEHHHHHHHCCCH
TQARLSIETELEPEWETVFSGLGWDDAALCCEPSVLIAEQTTTV
HHHEEEEECCCCCCHHHHHCCCCCCCCHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MNLDDDWLDMPEDKFNPTESDEQSEPAPELRVLYQDEHLVAIHKPAGLLVHRSYLARRER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHH
FFAMQLTRDLVGCHVFPVHRLDRPTSGVLLFAKSSEVANALCEQFANHSIEKQYLALVRG
HHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHC
NMHESGVLDYALKVELDEVADKFANQDKAAQDAVTQYEPLLNTEIPYPSGRYATSRFALV
CCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
KLSPKTGRKHQLRRHMAHLRHPIIGDTTHGDGKQNAFFRTHFDINRLWLIAKKLTFTHPV
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHEEHHHHHHHCCCH
TQARLSIETELEPEWETVFSGLGWDDAALCCEPSVLIAEQTTTV
HHHEEEEECCCCCCHHHHHCCCCCCCCHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]