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The map label for this gene is nagK [H]

Identifier: 117921139

GI number: 117921139

Start: 3224359

End: 3225285

Strand: Reverse

Name: nagK [H]

Synonym: Shewana3_2698

Alternate gene names: 117921139

Gene position: 3225285-3224359 (Counterclockwise)

Preceding gene: 117921140

Following gene: 117921138

Centisome position: 64.87

GC content: 54.05

Gene sequence:

>927_bases
ATGTATTACGGTTTAGATATAGGTGGAACAAAGATTGAGTTAGCGATTTTTGATACTCAATTAGCGCTACAGGATAAGTG
GCGTTTAAGTACTCCTGGGCAGGACTACAGTGCCTTTATGGCAACGCTGGCCGAGCAAATCGAAAAGGCGGATCAGCAAT
GTGGTGAGCGCGGTACCGTTGGGATTGCGCTCCCCGGGGTGGTGAAAGCCGACGGGACTGTGATTTCGAGTAATGTGCCT
TGCCTTAATCAACGCCGTGTTGCCCATGATCTTGCTCAGTTGCTCAATCGGACCGTGGCAATCGGTAATGATTGCCGCTG
TTTTGCCCTCTCCGAAGCGGTACTCGGAGTAGGCAGGGGCTACTCCCGCGTACTGGGGATGATTCTCGGCACGGGCACTG
GCGGGGGGTTATGTATCGATGGCAAGTTGTATCTTGGTGCAAACCGACTCGCCGGAGAGTTTGGTCATCAAGGCGTGAGC
GCCAATGTTGCCTGTCGACATCAACTGCCGCTCTATGTCTGTGGCTGCGGGCTTGAGGGCTGCGCCGAAACCTATGTGTC
AGGCACTGGACTTGGCAGACTCTACCAAGATATCGCGGGGCAAACGGCCGATACCTTTGCATGGCTGAATGCACTTCGGT
GCAACGACCCGCTCGCCATCAAAACCTTTGATACTTATATGGATATTCTCGGCAGCTTAATGGCTTCATTAGTGCTGGCG
ATGGACCCAGATATTATCGTGTTGGGCGGCGGATTATCCGAGGTGGAGGAAATTCTCGCTGCCTTACCCCAAGCGACCAA
GGCCCATCTTTTTGATGGCGTGACGTTGCCGCAATTTAAATTGGCCGACTTTGGTTCGGCCAGTGGCGTGCGCGGCGCGG
CTTTGCTCGGTCACGGGCTCGATGCGGGGATAAGCTATGAAGCCTAA

Upstream 100 bases:

>100_bases
TATCGCCAGATAATCCTTGTCCTACGGGGCAAGTTAATCGAGTTGTGCAGGGTGTCAATGTATATCCCTTTGTAAAATAA
ATATAAATAAGGGCAAGGGC

Downstream 100 bases:

>100_bases
TACGGATTTTATGCTGATAGCCGACGGCGCTAAGGTGCTAACTCAAGGCAATCTCACAGAGCACTGCGCGATTGAGGTGT
CGGATGGCATTATTTGCGGC

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MYYGLDIGGTKIELAIFDTQLALQDKWRLSTPGQDYSAFMATLAEQIEKADQQCGERGTVGIALPGVVKADGTVISSNVP
CLNQRRVAHDLAQLLNRTVAIGNDCRCFALSEAVLGVGRGYSRVLGMILGTGTGGGLCIDGKLYLGANRLAGEFGHQGVS
ANVACRHQLPLYVCGCGLEGCAETYVSGTGLGRLYQDIAGQTADTFAWLNALRCNDPLAIKTFDTYMDILGSLMASLVLA
MDPDIIVLGGGLSEVEEILAALPQATKAHLFDGVTLPQFKLADFGSASGVRGAALLGHGLDAGISYEA

Sequences:

>Translated_308_residues
MYYGLDIGGTKIELAIFDTQLALQDKWRLSTPGQDYSAFMATLAEQIEKADQQCGERGTVGIALPGVVKADGTVISSNVP
CLNQRRVAHDLAQLLNRTVAIGNDCRCFALSEAVLGVGRGYSRVLGMILGTGTGGGLCIDGKLYLGANRLAGEFGHQGVS
ANVACRHQLPLYVCGCGLEGCAETYVSGTGLGRLYQDIAGQTADTFAWLNALRCNDPLAIKTFDTYMDILGSLMASLVLA
MDPDIIVLGGGLSEVEEILAALPQATKAHLFDGVTLPQFKLADFGSASGVRGAALLGHGLDAGISYEA
>Mature_308_residues
MYYGLDIGGTKIELAIFDTQLALQDKWRLSTPGQDYSAFMATLAEQIEKADQQCGERGTVGIALPGVVKADGTVISSNVP
CLNQRRVAHDLAQLLNRTVAIGNDCRCFALSEAVLGVGRGYSRVLGMILGTGTGGGLCIDGKLYLGANRLAGEFGHQGVS
ANVACRHQLPLYVCGCGLEGCAETYVSGTGLGRLYQDIAGQTADTFAWLNALRCNDPLAIKTFDTYMDILGSLMASLVLA
MDPDIIVLGGGLSEVEEILAALPQATKAHLFDGVTLPQFKLADFGSASGVRGAALLGHGLDAGISYEA

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=39.7993311036789, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=36.7549668874172, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1790522, Length=262, Percent_Identity=27.8625954198473, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=22.7941176470588, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 32282; Mature: 32282

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGLDIGGTKIELAIFDTQLALQDKWRLSTPGQDYSAFMATLAEQIEKADQQCGERGTV
CEEEEECCCCEEEEEEEECEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
GIALPGVVKADGTVISSNVPCLNQRRVAHDLAQLLNRTVAIGNDCRCFALSEAVLGVGRG
EEECCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEHHHHHHHCCCC
YSRVLGMILGTGTGGGLCIDGKLYLGANRLAGEFGHQGVSANVACRHQLPLYVCGCGLEG
HHHHHHHHHCCCCCCCEEECCEEEECCHHHHHHHCCCCCCCCEEEEECCCEEEECCCCHH
CAETYVSGTGLGRLYQDIAGQTADTFAWLNALRCNDPLAIKTFDTYMDILGSLMASLVLA
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
MDPDIIVLGGGLSEVEEILAALPQATKAHLFDGVTLPQFKLADFGSASGVRGAALLGHGL
CCCCEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCCCC
DAGISYEA
CCCCCCCC
>Mature Secondary Structure
MYYGLDIGGTKIELAIFDTQLALQDKWRLSTPGQDYSAFMATLAEQIEKADQQCGERGTV
CEEEEECCCCEEEEEEEECEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
GIALPGVVKADGTVISSNVPCLNQRRVAHDLAQLLNRTVAIGNDCRCFALSEAVLGVGRG
EEECCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEHHHHHHHCCCC
YSRVLGMILGTGTGGGLCIDGKLYLGANRLAGEFGHQGVSANVACRHQLPLYVCGCGLEG
HHHHHHHHHCCCCCCCEEECCEEEECCHHHHHHHCCCCCCCCEEEEECCCEEEECCCCHH
CAETYVSGTGLGRLYQDIAGQTADTFAWLNALRCNDPLAIKTFDTYMDILGSLMASLVLA
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
MDPDIIVLGGGLSEVEEILAALPQATKAHLFDGVTLPQFKLADFGSASGVRGAALLGHGL
CCCCEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCCCC
DAGISYEA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA