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The map label for this gene is mdeA [H]

Identifier: 117921084

GI number: 117921084

Start: 3156336

End: 3157529

Strand: Reverse

Name: mdeA [H]

Synonym: Shewana3_2643

Alternate gene names: 117921084

Gene position: 3157529-3156336 (Counterclockwise)

Preceding gene: 117921085

Following gene: 117921083

Centisome position: 63.5

GC content: 50.67

Gene sequence:

>1194_bases
ATGCAAGATGAATCAAGCAAGCAGTGGAAAGCCGCGACCCAAGCTATTCATGCCGGGCATGAGCGAGAAGCTTTTGGTAC
TTTGGTGACGCCACTTTATCAAACGGCGACTTTTGTTTTTGAGTCGGCGCAGCAAGGCGGCGAGCGATTTGCCGGCAACG
AACCCGGTTATATTTATACTCGCCTAGGTAACCCGACCGTTGCCGAGCTGGAACGTAAAATGGCGATTTTAGAAGGCGCT
GAAGCGGCGGCAGCAACGGCCTCTGGCATGGGTGCTGTGTCGGCGGCGCTGCTGGCTAACTTGCAGATGGGCGATCATTT
AGTGGCTTCCAATGCGGTTTATGGTTGCACCTTTGCCCTTATGACTAGCCAATTTGCCCGCTTCGGTATCGAAGTCACCT
TAGTCGATTTTACCGATTTAGCGGCCATTGAACGGGCTATCAAACCCAACACTCGGGTGATTTTTTGCGAAACTCCGGTT
AATCCTCATTTACAGGTGTTTGATCTCAAAGGTATCGCCGATATTGCGAAACGGCATCAACTTGTCAGCATTGTCGATAA
TACTTTTATGACACCGTTACTGCAGCAACCCTTAGCATTCGGTATCGATTTAGTGGTACACAGTGCGACTAAGTATTTGA
ATGGGCACGGCGATGTAATTGCTGGCGTCGTGTGTGGCAGTGAAGAGCAGCTGCACAGAGTGAAATATGAGATTCTAAAG
GATATCGGTGCTGTGATGTCTCCCCACGATGCGTGGCTGATTTTGCGAGGGCTAAAAACCTTAGATGTGCGTTTGCAGCG
CCATTGTGACAGTGCGCAGCGTGTCGCCGAGTTTTTGGAGCAACATCCCGCAGTGACTCGGGTGTATTATCCCGGGCTTA
AATCCCATTCAGGGCATCGATTTATCGGTGGACAGATGGCGAAAGCTGGTGGCGTGATTGCCTTTGAGTTAGCCGCCTCT
CTTGAGCAAGCGATGGCTTTTGTGGGTTACCTTAAGCTGTTTTCAATCGCTGTAAGTTTGGGGGATGCGGAATCCCTTAT
TCAGCACCCAGCTTCCATGACCCACTCACCCTACACGCCAGAGGCACGCCAGGCAGCGGGAATAAGCGATAATTTACTGC
GCATCTCAATCGGTTTAGAGGATTGCGGCGATATCATCGAGGATTTAAACCAAGCTTTGGCGATGCTGGCGTAA

Upstream 100 bases:

>100_bases
AACTTATCAATAGGACTTATCAAGATGCTAAGCATGGTCTCATTCAAACAAACTATGAAACAACAAGCGAATGAGGCACT
GCGACAACAAGGAGTGAGCA

Downstream 100 bases:

>100_bases
TGGTTTGTGGTACTTCGCGAAAGGATGCCTTAACGGCGTCCTTTTGGCTTTCTAGAAAGGATTAACCCGCAAGGGAACAG
GCTATTTTAGTCATCGATAA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQDESSKQWKAATQAIHAGHEREAFGTLVTPLYQTATFVFESAQQGGERFAGNEPGYIYTRLGNPTVAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTFALMTSQFARFGIEVTLVDFTDLAAIERAIKPNTRVIFCETPV
NPHLQVFDLKGIADIAKRHQLVSIVDNTFMTPLLQQPLAFGIDLVVHSATKYLNGHGDVIAGVVCGSEEQLHRVKYEILK
DIGAVMSPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLEQHPAVTRVYYPGLKSHSGHRFIGGQMAKAGGVIAFELAAS
LEQAMAFVGYLKLFSIAVSLGDAESLIQHPASMTHSPYTPEARQAAGISDNLLRISIGLEDCGDIIEDLNQALAMLA

Sequences:

>Translated_397_residues
MQDESSKQWKAATQAIHAGHEREAFGTLVTPLYQTATFVFESAQQGGERFAGNEPGYIYTRLGNPTVAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTFALMTSQFARFGIEVTLVDFTDLAAIERAIKPNTRVIFCETPV
NPHLQVFDLKGIADIAKRHQLVSIVDNTFMTPLLQQPLAFGIDLVVHSATKYLNGHGDVIAGVVCGSEEQLHRVKYEILK
DIGAVMSPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLEQHPAVTRVYYPGLKSHSGHRFIGGQMAKAGGVIAFELAAS
LEQAMAFVGYLKLFSIAVSLGDAESLIQHPASMTHSPYTPEARQAAGISDNLLRISIGLEDCGDIIEDLNQALAMLA
>Mature_397_residues
MQDESSKQWKAATQAIHAGHEREAFGTLVTPLYQTATFVFESAQQGGERFAGNEPGYIYTRLGNPTVAELERKMAILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTFALMTSQFARFGIEVTLVDFTDLAAIERAIKPNTRVIFCETPV
NPHLQVFDLKGIADIAKRHQLVSIVDNTFMTPLLQQPLAFGIDLVVHSATKYLNGHGDVIAGVVCGSEEQLHRVKYEILK
DIGAVMSPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLEQHPAVTRVYYPGLKSHSGHRFIGGQMAKAGGVIAFELAAS
LEQAMAFVGYLKLFSIAVSLGDAESLIQHPASMTHSPYTPEARQAAGISDNLLRISIGLEDCGDIIEDLNQALAMLA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=370, Percent_Identity=40.5405405405405, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI299473759, Length=370, Percent_Identity=37.8378378378378, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI25453487, Length=369, Percent_Identity=35.5013550135501, Blast_Score=212, Evalue=5e-55,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=39.6373056994819, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1789383, Length=400, Percent_Identity=31.25, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=40.9090909090909, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=41.2371134020619, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17538055, Length=388, Percent_Identity=39.9484536082474, Blast_Score=270, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=41.1167512690355, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=32.1759259259259, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=29.3532338308458, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=32, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=405, Percent_Identity=27.9012345679012, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=38.659793814433, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42888; Mature: 42888

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDESSKQWKAATQAIHAGHEREAFGTLVTPLYQTATFVFESAQQGGERFAGNEPGYIYT
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RLGNPTVAELERKMAILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTFAL
ECCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MTSQFARFGIEVTLVDFTDLAAIERAIKPNTRVIFCETPVNPHLQVFDLKGIADIAKRHQ
HHHHHHHCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHH
LVSIVDNTFMTPLLQQPLAFGIDLVVHSATKYLNGHGDVIAGVVCGSEEQLHRVKYEILK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVMSPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLEQHPAVTRVYYPGLKSHSGHR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
FIGGQMAKAGGVIAFELAASLEQAMAFVGYLKLFSIAVSLGDAESLIQHPASMTHSPYTP
ECCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARQAAGISDNLLRISIGLEDCGDIIEDLNQALAMLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDESSKQWKAATQAIHAGHEREAFGTLVTPLYQTATFVFESAQQGGERFAGNEPGYIYT
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RLGNPTVAELERKMAILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTFAL
ECCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MTSQFARFGIEVTLVDFTDLAAIERAIKPNTRVIFCETPVNPHLQVFDLKGIADIAKRHQ
HHHHHHHCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHH
LVSIVDNTFMTPLLQQPLAFGIDLVVHSATKYLNGHGDVIAGVVCGSEEQLHRVKYEILK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVMSPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLEQHPAVTRVYYPGLKSHSGHR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
FIGGQMAKAGGVIAFELAASLEQAMAFVGYLKLFSIAVSLGDAESLIQHPASMTHSPYTP
ECCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARQAAGISDNLLRISIGLEDCGDIIEDLNQALAMLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]