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The map label for this gene is pinE [H]

Identifier: 117921078

GI number: 117921078

Start: 3147377

End: 3147925

Strand: Reverse

Name: pinE [H]

Synonym: Shewana3_2637

Alternate gene names: 117921078

Gene position: 3147925-3147377 (Counterclockwise)

Preceding gene: 117921079

Following gene: 117921077

Centisome position: 63.31

GC content: 43.53

Gene sequence:

>549_bases
ATGGCTCTAATTGGCTATGCTAGGGTATCAACTCAAGACCAGTCTCTGGATGCACAAATTGACGCGCTTACCAATGCGGG
TTGCGTGAAGATTTATCACGGTAAGCAATCAGGTAAGTCGGATGATAATTGGAAGAAGTTAGATGAGCTTATAGATTACT
CTAGGGAAGGGGATGTTGTGGTTGTAACCGCCTTTGACCGACTAGGCCGAAGTCTTAATCAGGTGTTGTCTAAAGTGGAT
GCTTTAAGTAAGAAAGGGGTTCATATAAAGACTTTAGACGGGCAAATAGACACAAGTAATGGCTCGCCTATGGCTAAGGC
ATTTACACAGCTTCTAGGGGTGTTTGCGGAGCTTGAGCACTCGATAATTACAGATAGATTGCAGTCTGGAAGGGTGCGAA
CTGGAAGGCTTGGAGGAAGAACTCCCAAGTTAAGTCCTGAGCAACGAAATCAAGTGAGAACACGCTTAAAGAATGGTGAA
AGCAAGCTCAAGCTATCTAAAGAGTTCTTGGTAAGTAGAGCTACCATTCTGAATATTGAGAATGAATAG

Upstream 100 bases:

>100_bases
ACCTTACACTGTTAATATTTTAAAAACCAAAGTTATTGACGCCAATAAATCACGCTGCCAATATCTATGTAGTTTTTAAC
ACTCTCATAAGGTGATTTAA

Downstream 100 bases:

>100_bases
TGTGTTTAGACTGTGTTTAAAGGGATTTTCAGATAGGGTTAAAGCTGGCTTATCACATGGTGTTATGTGGTTTGCTGCAT
CAGGTCTTTTAATGCTTTCC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MALIGYARVSTQDQSLDAQIDALTNAGCVKIYHGKQSGKSDDNWKKLDELIDYSREGDVVVVTAFDRLGRSLNQVLSKVD
ALSKKGVHIKTLDGQIDTSNGSPMAKAFTQLLGVFAELEHSIITDRLQSGRVRTGRLGGRTPKLSPEQRNQVRTRLKNGE
SKLKLSKEFLVSRATILNIENE

Sequences:

>Translated_182_residues
MALIGYARVSTQDQSLDAQIDALTNAGCVKIYHGKQSGKSDDNWKKLDELIDYSREGDVVVVTAFDRLGRSLNQVLSKVD
ALSKKGVHIKTLDGQIDTSNGSPMAKAFTQLLGVFAELEHSIITDRLQSGRVRTGRLGGRTPKLSPEQRNQVRTRLKNGE
SKLKLSKEFLVSRATILNIENE
>Mature_181_residues
ALIGYARVSTQDQSLDAQIDALTNAGCVKIYHGKQSGKSDDNWKKLDELIDYSREGDVVVVTAFDRLGRSLNQVLSKVDA
LSKKGVHIKTLDGQIDTSNGSPMAKAFTQLLGVFAELEHSIITDRLQSGRVRTGRLGGRTPKLSPEQRNQVRTRLKNGES
KLKLSKEFLVSRATILNIENE

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=38.2022471910112, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1787827, Length=184, Percent_Identity=32.0652173913043, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1787638, Length=184, Percent_Identity=32.0652173913043, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20096; Mature: 19965

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIGYARVSTQDQSLDAQIDALTNAGCVKIYHGKQSGKSDDNWKKLDELIDYSREGDVV
CCEECCEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEE
VVTAFDRLGRSLNQVLSKVDALSKKGVHIKTLDGQIDTSNGSPMAKAFTQLLGVFAELEH
EEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHH
SIITDRLQSGRVRTGRLGGRTPKLSPEQRNQVRTRLKNGESKLKLSKEFLVSRATILNIE
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEC
NE
CC
>Mature Secondary Structure 
ALIGYARVSTQDQSLDAQIDALTNAGCVKIYHGKQSGKSDDNWKKLDELIDYSREGDVV
CEECCEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEE
VVTAFDRLGRSLNQVLSKVDALSKKGVHIKTLDGQIDTSNGSPMAKAFTQLLGVFAELEH
EEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHH
SIITDRLQSGRVRTGRLGGRTPKLSPEQRNQVRTRLKNGESKLKLSKEFLVSRATILNIE
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEC
NE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]