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The map label for this gene is asnB [H]

Identifier: 117920990

GI number: 117920990

Start: 3047610

End: 3049274

Strand: Direct

Name: asnB [H]

Synonym: Shewana3_2549

Alternate gene names: 117920990

Gene position: 3047610-3049274 (Clockwise)

Preceding gene: 117920989

Following gene: 117920994

Centisome position: 61.29

GC content: 51.35

Gene sequence:

>1665_bases
ATGTGTTCGATTTTTTCAATTTTAGATATTCAATCCGATGCGAAAGAGCTGCGCCAAGTGGCACTGGAAATGTCCAAACT
GATGCGCCATCGCGGCCCCGATTGGTCAGGCATTTACGCCAGCGACAAAGCGATTTTGGCCCATGAGCGCTTGGCGATTG
TCGATATTGAACACGGTGCGCAGCCACTGCTAACCGAAGACGGCAGCCTTATCCTCGCGGTAAACGGTGAGATCTACAAC
CATAAAGAACTCAAAGCCCAACTCGGCGATAAATACAGCTATCAAACCAACTCTGACTGCGAAGTGATCTTGGCGCTATA
TCAAGAATATGGCACTGAATTCCTTGATAAATTAAACGGTATTTTCGCCTTCGTACTCTACGATAAAGCCAAAGACGCCT
ACCTTATTGGCCGCGATCATATGGGCATTATCCCACTGTACACAGGGCGTGATGCGGCGGGTAACTTCTATGTCGCCTCC
GAAATGAAGGCACTGATGCCTGTCTGTAAAACCGTGGCCGAATTCCAGCCAGGGCAATATCTGTACTCAAGCATCGGTGA
GCCAGTGCAATACTACACTCGCGATTGGCAGAGCTTTGATGCAGTGAAAGATAACGGCGCCAGCCAAGAAGAATTACGTG
ATGCCCTCGAAGCCGCCGTTAAGCGCCAATTGATGTCCGACGTGCCCTACGGCGTGTTGTTGTCTGGCGGTTTAGATTCC
TCGGTGATCTCAGCCATTACCCAAACCTTTGCCAAGCGCCGTATTGAAGATGACGATCAAAGTGGCGCTTGGTGGCCACA
GCTGCACTCCTTTGCCGTAGGGTTAAAAGGCGCGCCGGATTTAGCCGCCGCTAAGAAGGTCGCCGATGCGATTGGTACCA
TTCACCACGAGATCAACTTTACCTTCCAAGAGGGTTTAGATGCGATTAAAGAAGTCATCTACCACTTAGAAACCTATGAT
GTGACCACCATTCGTGCCGCGACACCTATGTACCTGATGGCGCGTAAAATCAAAGCGATGGGCATTAAAATGGTGTTATC
GGGTGAAGGTGCCGACGAACTCTTTGGCGGTTACTTGTACTTCCATAAGGCACCAAACGCGCAGGCCTTCCATGAAGAAC
TCGTGCGTAAACTGGATAAACTGCATCTGTTCGACTGTCTGCGCGCCAACAAAGCCATGGCGGCGTGGGGACTCGAAGCC
CGTGTGCCTTTCCTCGATAAGGAATTTATGGATGTGGCGATGCGCATCAACCCCGAGGCAAAAATGTCTAAGGATGGCCG
CATTGAGAAACACATTCTGCGCCAAGCCTTTGAGCATAAACTGCCAAAAGAAGTCGCCTGGCGTCAAAAGGAGCAATTCA
GCGATGGCGTGGGTTACTCTTGGATCGATGGTTTAAAGGCCCATGCTGCTGAGCAAGTCGACGACTTACAACTGGCTAAC
GCGAAGTTCCGCTTCCCGTACAACACGCCAGAAACCAAGGAAGCCTATTTCTACCGTTGTTTCTTTGAGGAGCATTATCC
ATTGCCAAGCGCCGCCGAAACGGTTCCCGGTGGCAAGTCGGTCGCCTGCTCTACTCCAGAAGCGCTGGCATGGGATGAAA
GCCTACGTGGCATTATCGACCCCTCTGGCCGCGCCGTACGCTCAGTGCATGCCGCCAGCTATTAA

Upstream 100 bases:

>100_bases
AATCGTCAAAAATAATGTTTGGCAAAATCCCGAATTGCTGGCACATTGATACTCAATTGAGACATCCTCAAATGAAATAT
AGCGGTTTAGGAGTTGCATT

Downstream 100 bases:

>100_bases
TCGCTGACGCATGCCATCAAGATAAAAGCCACCCTAGGGTGGCTTTTATCTTTAACTATCTAACTCTTTAAGACTTGGAT
TTAGCTTGACCACATGGCGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAKDAYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVAEFQPGQYLYSSIGEPVQYYTRDWQSFDAVKDNGASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDDQSGAWWPQLHSFAVGLKGAPDLAAAKKVADAIGTIHHEINFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKAHAAEQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLPSAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHAASY

Sequences:

>Translated_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAKDAYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVAEFQPGQYLYSSIGEPVQYYTRDWQSFDAVKDNGASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDDQSGAWWPQLHSFAVGLKGAPDLAAAKKVADAIGTIHHEINFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKAHAAEQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLPSAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHAASY
>Mature_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAKDAYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVAEFQPGQYLYSSIGEPVQYYTRDWQSFDAVKDNGASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SVISAITQTFAKRRIEDDDQSGAWWPQLHSFAVGLKGAPDLAAAKKVADAIGTIHHEINFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKAHAAEQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLPSAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHAASY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=40.8839779005525, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=40.8839779005525, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=40.8839779005525, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=42.4124513618677, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=43.4298440979955, Blast_Score=311, Evalue=9e-85,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=43.4298440979955, Blast_Score=311, Evalue=9e-85,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.7581227436823, Blast_Score=834, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=561, Percent_Identity=42.2459893048128, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71993933, Length=531, Percent_Identity=43.3145009416196, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=35.9464627151052, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI25147560, Length=312, Percent_Identity=38.7820512820513, Blast_Score=184, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.6422018348624, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=50.1831501831502, Blast_Score=522, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=46.2738301559792, Blast_Score=497, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62090; Mature: 62090

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IFAFVLYDKAKDAYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVAEFQPGQY
EEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHH
LYSSIGEPVQYYTRDWQSFDAVKDNGASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
HHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDDQSGAWWPQLHSFAVGLKGAPDLAAAKKVADAIGTIHHEINF
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKAHAAEQVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLPSAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCHHEEEEEEHHCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHAASY
CCCHHHHHHHHCCC
>Mature Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IFAFVLYDKAKDAYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVAEFQPGQY
EEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHH
LYSSIGEPVQYYTRDWQSFDAVKDNGASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
HHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAITQTFAKRRIEDDDQSGAWWPQLHSFAVGLKGAPDLAAAKKVADAIGTIHHEINF
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRQAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKAHAAEQVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLPSAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCHHEEEEEEHHCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHAASY
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]