Click here to switch to the map view.

The map label for this gene is dusC [H]

Identifier: 117920895

GI number: 117920895

Start: 2940208

End: 2941155

Strand: Direct

Name: dusC [H]

Synonym: Shewana3_2452

Alternate gene names: 117920895

Gene position: 2940208-2941155 (Clockwise)

Preceding gene: 117920880

Following gene: 117920896

Centisome position: 59.13

GC content: 54.64

Gene sequence:

>948_bases
GTGCGCGTAATTTTGGCTCCGATGGAAGGCGTGGTTGACGACTTAATGCGAGATATTTTGTCCTCAATTAACCCCTACGA
TTTGCTCGTCACCGAATTTGTGCGCGTGGTTGACCAACTCTTACCCGAAAAAGTGTTCCTCAAACTCTGCCCCGAACTGC
TCAATGGCGGCTTTACCCCTTCGGGCACGCCGGTTCGCGTACAGCTTTTAGGGCAAGAGCCCAACTGCATGGCCGAAAAC
GCAATGCGCGCGATTGAGCTTGGCTCCCACGGCGTCGATGCTAACTTTGGTTGCCCCGCTAAGATGGTGAACCGCAGTAA
TGGCGGCGCGGTGTTGCTGCAATACCCTAATACGATTCACGATATTGTGCGCGCCATGCGCCAAGCCGTGCCCGCCGAGC
ATCCGGTGACGGCGAAGATTCGTTTAGGTTACGAAGACAAATCCCTGTTTATGGAGAATGCGCTAGCCGTTTATGAAGCG
GGCGCGACTGAGCTGGCTATCCATGCCCGCAGTAAGGTCGATGGTTATAAACCTCCCGCCTACTGGGAATACATCACCGA
AGTGCGCGAGCGTTTGCCCATTCCAGTCATTGCCAATGGTGAGATTTGGAACCGCGAGGATGCAAAGCGTTGTATGGCGG
TGACGGGCTGCGACAGCATTATGATTGGCCGCGGCGCCATTTCGCTGCCCAATCTTGCCGATACCATTAAGACTGGCGCG
GCCCCCTACAGCTGGGCCGACACCTTAGGCTTGATGCTAAGCTACACCCAACGTGAACTGAGCGGCCGTAAGAGTGATTA
TTATCCGGCGCGAATTAAGCAGTGGTTTAGTTACTTAAATCGCCAATACCCCGAGGCGGACACCCTGTTTAGGGAGCTGC
GCATCTATAAGACCACTGAGGAAATTGTGCGAGTGCTGGAAGAGGCACAGCAGCGCCTTGGCGAGTAA

Upstream 100 bases:

>100_bases
TAACCCAAAACAGTCATCGCCCTTCCCTCGCAATGCTTTGTAACCACAGTCCAATAGGATAGAATGCACGCCCTTTGAAA
CTGGATATGGGTTTGTTGCA

Downstream 100 bases:

>100_bases
ACACGGATGGTGCATAGGATAATCCCATGAGAAAGTGGTGAATTTTTATTTAAAACACCACTAAATCCCTGCAAATCGGA
CAGATACTTGATCTTTATCA

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNGGFTPSGTPVRVQLLGQEPNCMAEN
AMRAIELGSHGVDANFGCPAKMVNRSNGGAVLLQYPNTIHDIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEA
GATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNREDAKRCMAVTGCDSIMIGRGAISLPNLADTIKTGA
APYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNRQYPEADTLFRELRIYKTTEEIVRVLEEAQQRLGE

Sequences:

>Translated_315_residues
MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNGGFTPSGTPVRVQLLGQEPNCMAEN
AMRAIELGSHGVDANFGCPAKMVNRSNGGAVLLQYPNTIHDIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEA
GATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNREDAKRCMAVTGCDSIMIGRGAISLPNLADTIKTGA
APYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNRQYPEADTLFRELRIYKTTEEIVRVLEEAQQRLGE
>Mature_315_residues
MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNGGFTPSGTPVRVQLLGQEPNCMAEN
AMRAIELGSHGVDANFGCPAKMVNRSNGGAVLLQYPNTIHDIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEA
GATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNREDAKRCMAVTGCDSIMIGRGAISLPNLADTIKTGA
APYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNRQYPEADTLFRELRIYKTTEEIVRVLEEAQQRLGE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=257, Percent_Identity=31.5175097276265, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=33.1877729257642, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI40807366, Length=257, Percent_Identity=29.5719844357977, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI31742496, Length=172, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI8923374, Length=259, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=53.2467532467532, Blast_Score=341, Evalue=3e-95,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=30.4075235109718, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI145693211, Length=281, Percent_Identity=27.0462633451957, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=171, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=158, Percent_Identity=34.1772151898734, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=170, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=179, Percent_Identity=31.2849162011173, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=223, Percent_Identity=23.7668161434978, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=32.9059829059829, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24580595, Length=191, Percent_Identity=30.3664921465969, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=191, Percent_Identity=30.3664921465969, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=148, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549423, Length=156, Percent_Identity=30.1282051282051, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35326; Mature: 35326

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNGGFTP
CEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTPVRVQLLGQEPNCMAENAMRAIELGSHGVDANFGCPAKMVNRSNGGAVLLQYPNTIH
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCHHH
DIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEAGATELAIHARSKVDGYKPPA
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCCH
YWEYITEVRERLPIPVIANGEIWNREDAKRCMAVTGCDSIMIGRGAISLPNLADTIKTGA
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCC
APYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNRQYPEADTLFRELRIYKTTE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIVRVLEEAQQRLGE
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKVFLKLCPELLNGGFTP
CEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTPVRVQLLGQEPNCMAENAMRAIELGSHGVDANFGCPAKMVNRSNGGAVLLQYPNTIH
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCHHH
DIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEAGATELAIHARSKVDGYKPPA
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCCH
YWEYITEVRERLPIPVIANGEIWNREDAKRCMAVTGCDSIMIGRGAISLPNLADTIKTGA
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCC
APYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNRQYPEADTLFRELRIYKTTE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIVRVLEEAQQRLGE
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]