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The map label for this gene is pleD [H]

Identifier: 117920878

GI number: 117920878

Start: 2919485

End: 2921665

Strand: Direct

Name: pleD [H]

Synonym: Shewana3_2435

Alternate gene names: 117920878

Gene position: 2919485-2921665 (Clockwise)

Preceding gene: 117920877

Following gene: 117920880

Centisome position: 58.72

GC content: 45.39

Gene sequence:

>2181_bases
TTGCGTGTATCAGCTCTGAATATTGCCCATCTAAAGTTAGCCACCTTTCTCGGCCTAGTGGGTTTAGTGCTCAACATCTA
CCCTATTCCACTTTTTGCCAACGTCCAGCTTATTTTAGGCAACGCGGCGGTGGTGATTGTTGCCATCTTACTCGGGCCTT
GGTATGCCCTGTTTACTGCCCTATTTACGGCAACGGGTTTGATGTTAGCTTGGTCTAGTGCCCACGTGTATCTGGTGTTT
TTGCTTGAGGCGCTGTGGTTGGGGTTTGCGCGGCGCAGAGATTTCCCCATACTCTACGCCAGCATTTGTTATTGGCTGTT
GTTAGGCTTGCCATTAATGGGCTTATACCTGTGGTTAATCACGGGGATGCCGACTTACCACATTCCCTTTACCACGGTAA
AACAAGCTTTTAATGGCATTTTCTACACTACCTTAGGTGAGCTTTGTGTGGTGGCACTCCCCAGTTTGTGGCACTTAAAA
GATAAGCTGGTAAACCATACTCGCCGCACCATGAGTGCGCAGCTTAGCTATCTGTTTATTTTGATTACCACCATCAGCTT
ACTGACCTCATCCCTCGTGTTTAATCATTATTTTATGGATAAACAACAGGTGTTGATTAATCAAAACCTCAATGACACAG
GGATCTCCCTTGGCCACGCCACCGAAACTTATATCAGCACTAACACCCGTACTATTGCCAGCATAGGTAAATTTATCAGC
GTATCGGGTTTGCCATTCGAACAATGGCAACTCGTCCTCGATAGTGTTCAAGGGTTAACCCCCAGTTTTACGACTATGTT
TATCGCCAATGAAGATGGCGACATAGTGGCGGGCAGCCCACTTGAGAAACTTAACCAAGTTAGGCTGTTACCACAAAAAG
TAAATGTGCTAGATAGGGATTACTTTATCCAAGCTTTTATTCACCATAACACCTTTGTCTCGCCAGTATTTATTGGCCGT
GGGCTCGGCAAGGACATCATCATCGCCATCAGTGCCCCTATCTTTAAAGAAGAGTCTAAAACTGCGATTGGTATTGTTGA
GGGCAGCCTCGATTTAAGTTACTTCTCCAATATTGACAAGCAAAATCAACACCATGAGACACAATCCATTATCTTATTGG
ATGAGAAGAAAAATATTGTTTACGCCTCGGAAAGGCTCGGGCTACAACCCTTAACCCCTTTCAACTATGTCACGGGTAGC
ACTGAGTATCGCACGCGATTACAGCTGATGAATATCAATCAGGAGGATACCGAAACGCCTGAATATATTTACGCCCACCA
TAAACTCAAAAATGGTTGGCATTTGTATGTGGTTGAGCCGTTTATCCCTCTGCTTACCTTAGCCCAAGAGCAATATACTA
AGACCATAGTGCTACTGTTACTCAGTATGGTCGGCGCCATTATTATCGCCAAAGCAATCAGCCGTTTAACGACTACTCCC
CTCGCCTTACTGGCGCAACATTTTAGCCAAAAGAAAGACGGCAGTATCAATGAGGAAAAATTTGAACACGAGCTACTCGA
TAACAGTACGCCAAAGGAAATCTATAGCCTGTACGAGAGCTTAGAAGCCAATCAGCAAGTGCTGCTTAGCCATCAACTCG
AGTTAGAAGATAAAGTAAAACAGCGCACTATGGATTTAGAAATCGCTAACCGCAAACTCAAGGATATGGCCGAGCGGGAT
TCCCTCACCAATTTGTACAATCGACGTTATACCGAAAAGCAATTCCTGAAAATTCAAGACTTGTGTGAGCGAGGCCAAGA
TGCCATTGCCGTAGTATTGCTCGACCTCGACTTCTTTAAAAAAGTGAATGACACCTACGGCCATCTGGCGGGCGATGAAT
GCCTACGGGTGATGGCTGAAGTGTTAACGAGTCACTTTAAGCGTGATGTCGATTTGCTGTGTCGTTATGGCGGCGAAGAG
TTCGTCTTGATATTACCTATGTGTAATACCTTGAAAGTTGAGCAACATCTTAATGGCTTCCGCGAAAAGCTCGCCAGCGT
GGTGATTACGAACCCAGCCGATCAAGTCACTTTTAGTGTCACAGTCAGCATAGGCGCCATCATTGCCGATGCCGCTTACA
GCTCAACGCTTGAAGTTTGGTTAAAACAAGCGGATGAGAACCTCTATAAAGCTAAGGAACAAGGGCGCAACTGCGTGGTT
TGCACCCTAGTCACAGTTTAA

Upstream 100 bases:

>100_bases
CAATATCAAATTAAGAAACTTCTGCTAAGCTAGATATAATTTATAGTTTCAGTGCAATCGATTGCTTTTTAAGCATAATT
TTGACTAAAGGGACTTATCT

Downstream 100 bases:

>100_bases
TCGGGCATATGCGCGGTGCGATATTGGTCAATCCGCGTCAGCATATAGGGGAAAAACGGATTCCATTTTTGGCGTAACAT
TTCCGCGGCGGGCACGCTAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MRVSALNIAHLKLATFLGLVGLVLNIYPIPLFANVQLILGNAAVVIVAILLGPWYALFTALFTATGLMLAWSSAHVYLVF
LLEALWLGFARRRDFPILYASICYWLLLGLPLMGLYLWLITGMPTYHIPFTTVKQAFNGIFYTTLGELCVVALPSLWHLK
DKLVNHTRRTMSAQLSYLFILITTISLLTSSLVFNHYFMDKQQVLINQNLNDTGISLGHATETYISTNTRTIASIGKFIS
VSGLPFEQWQLVLDSVQGLTPSFTTMFIANEDGDIVAGSPLEKLNQVRLLPQKVNVLDRDYFIQAFIHHNTFVSPVFIGR
GLGKDIIIAISAPIFKEESKTAIGIVEGSLDLSYFSNIDKQNQHHETQSIILLDEKKNIVYASERLGLQPLTPFNYVTGS
TEYRTRLQLMNINQEDTETPEYIYAHHKLKNGWHLYVVEPFIPLLTLAQEQYTKTIVLLLLSMVGAIIIAKAISRLTTTP
LALLAQHFSQKKDGSINEEKFEHELLDNSTPKEIYSLYESLEANQQVLLSHQLELEDKVKQRTMDLEIANRKLKDMAERD
SLTNLYNRRYTEKQFLKIQDLCERGQDAIAVVLLDLDFFKKVNDTYGHLAGDECLRVMAEVLTSHFKRDVDLLCRYGGEE
FVLILPMCNTLKVEQHLNGFREKLASVVITNPADQVTFSVTVSIGAIIADAAYSSTLEVWLKQADENLYKAKEQGRNCVV
CTLVTV

Sequences:

>Translated_726_residues
MRVSALNIAHLKLATFLGLVGLVLNIYPIPLFANVQLILGNAAVVIVAILLGPWYALFTALFTATGLMLAWSSAHVYLVF
LLEALWLGFARRRDFPILYASICYWLLLGLPLMGLYLWLITGMPTYHIPFTTVKQAFNGIFYTTLGELCVVALPSLWHLK
DKLVNHTRRTMSAQLSYLFILITTISLLTSSLVFNHYFMDKQQVLINQNLNDTGISLGHATETYISTNTRTIASIGKFIS
VSGLPFEQWQLVLDSVQGLTPSFTTMFIANEDGDIVAGSPLEKLNQVRLLPQKVNVLDRDYFIQAFIHHNTFVSPVFIGR
GLGKDIIIAISAPIFKEESKTAIGIVEGSLDLSYFSNIDKQNQHHETQSIILLDEKKNIVYASERLGLQPLTPFNYVTGS
TEYRTRLQLMNINQEDTETPEYIYAHHKLKNGWHLYVVEPFIPLLTLAQEQYTKTIVLLLLSMVGAIIIAKAISRLTTTP
LALLAQHFSQKKDGSINEEKFEHELLDNSTPKEIYSLYESLEANQQVLLSHQLELEDKVKQRTMDLEIANRKLKDMAERD
SLTNLYNRRYTEKQFLKIQDLCERGQDAIAVVLLDLDFFKKVNDTYGHLAGDECLRVMAEVLTSHFKRDVDLLCRYGGEE
FVLILPMCNTLKVEQHLNGFREKLASVVITNPADQVTFSVTVSIGAIIADAAYSSTLEVWLKQADENLYKAKEQGRNCVV
CTLVTV
>Mature_726_residues
MRVSALNIAHLKLATFLGLVGLVLNIYPIPLFANVQLILGNAAVVIVAILLGPWYALFTALFTATGLMLAWSSAHVYLVF
LLEALWLGFARRRDFPILYASICYWLLLGLPLMGLYLWLITGMPTYHIPFTTVKQAFNGIFYTTLGELCVVALPSLWHLK
DKLVNHTRRTMSAQLSYLFILITTISLLTSSLVFNHYFMDKQQVLINQNLNDTGISLGHATETYISTNTRTIASIGKFIS
VSGLPFEQWQLVLDSVQGLTPSFTTMFIANEDGDIVAGSPLEKLNQVRLLPQKVNVLDRDYFIQAFIHHNTFVSPVFIGR
GLGKDIIIAISAPIFKEESKTAIGIVEGSLDLSYFSNIDKQNQHHETQSIILLDEKKNIVYASERLGLQPLTPFNYVTGS
TEYRTRLQLMNINQEDTETPEYIYAHHKLKNGWHLYVVEPFIPLLTLAQEQYTKTIVLLLLSMVGAIIIAKAISRLTTTP
LALLAQHFSQKKDGSINEEKFEHELLDNSTPKEIYSLYESLEANQQVLLSHQLELEDKVKQRTMDLEIANRKLKDMAERD
SLTNLYNRRYTEKQFLKIQDLCERGQDAIAVVLLDLDFFKKVNDTYGHLAGDECLRVMAEVLTSHFKRDVDLLCRYGGEE
FVLILPMCNTLKVEQHLNGFREKLASVVITNPADQVTFSVTVSIGAIIADAAYSSTLEVWLKQADENLYKAKEQGRNCVV
CTLVTV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=36.6279069767442, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=32.6086956521739, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=30.9782608695652, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=36.7816091954023, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=35.2272727272727, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=33.1288343558282, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=35, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=35.7541899441341, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=32.9113924050633, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 81973; Mature: 81973

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSALNIAHLKLATFLGLVGLVLNIYPIPLFANVQLILGNAAVVIVAILLGPWYALFTA
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCHHHHHHHHHHHHHHHHHHH
LFTATGLMLAWSSAHVYLVFLLEALWLGFARRRDFPILYASICYWLLLGLPLMGLYLWLI
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGMPTYHIPFTTVKQAFNGIFYTTLGELCVVALPSLWHLKDKLVNHTRRTMSAQLSYLFI
HCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LITTISLLTSSLVFNHYFMDKQQVLINQNLNDTGISLGHATETYISTNTRTIASIGKFIS
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHC
VSGLPFEQWQLVLDSVQGLTPSFTTMFIANEDGDIVAGSPLEKLNQVRLLPQKVNVLDRD
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHHHHHH
YFIQAFIHHNTFVSPVFIGRGLGKDIIIAISAPIFKEESKTAIGIVEGSLDLSYFSNIDK
HHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCHHHHHCCCC
QNQHHETQSIILLDEKKNIVYASERLGLQPLTPFNYVTGSTEYRTRLQLMNINQEDTETP
CCCCCCCCEEEEEECCCCEEEEHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCC
EYIYAHHKLKNGWHLYVVEPFIPLLTLAQEQYTKTIVLLLLSMVGAIIIAKAISRLTTTP
HHEEEHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LALLAQHFSQKKDGSINEEKFEHELLDNSTPKEIYSLYESLEANQQVLLSHQLELEDKVK
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
QRTMDLEIANRKLKDMAERDSLTNLYNRRYTEKQFLKIQDLCERGQDAIAVVLLDLDFFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHH
KVNDTYGHLAGDECLRVMAEVLTSHFKRDVDLLCRYGGEEFVLILPMCNTLKVEQHLNGF
HHCHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
REKLASVVITNPADQVTFSVTVSIGAIIADAAYSSTLEVWLKQADENLYKAKEQGRNCVV
HHHHHHEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CTLVTV
EEEEEC
>Mature Secondary Structure
MRVSALNIAHLKLATFLGLVGLVLNIYPIPLFANVQLILGNAAVVIVAILLGPWYALFTA
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCHHHHHHHHHHHHHHHHHHH
LFTATGLMLAWSSAHVYLVFLLEALWLGFARRRDFPILYASICYWLLLGLPLMGLYLWLI
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGMPTYHIPFTTVKQAFNGIFYTTLGELCVVALPSLWHLKDKLVNHTRRTMSAQLSYLFI
HCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LITTISLLTSSLVFNHYFMDKQQVLINQNLNDTGISLGHATETYISTNTRTIASIGKFIS
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHHHC
VSGLPFEQWQLVLDSVQGLTPSFTTMFIANEDGDIVAGSPLEKLNQVRLLPQKVNVLDRD
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHHHHHH
YFIQAFIHHNTFVSPVFIGRGLGKDIIIAISAPIFKEESKTAIGIVEGSLDLSYFSNIDK
HHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCHHHHHCCCC
QNQHHETQSIILLDEKKNIVYASERLGLQPLTPFNYVTGSTEYRTRLQLMNINQEDTETP
CCCCCCCCEEEEEECCCCEEEEHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCC
EYIYAHHKLKNGWHLYVVEPFIPLLTLAQEQYTKTIVLLLLSMVGAIIIAKAISRLTTTP
HHEEEHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LALLAQHFSQKKDGSINEEKFEHELLDNSTPKEIYSLYESLEANQQVLLSHQLELEDKVK
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
QRTMDLEIANRKLKDMAERDSLTNLYNRRYTEKQFLKIQDLCERGQDAIAVVLLDLDFFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHH
KVNDTYGHLAGDECLRVMAEVLTSHFKRDVDLLCRYGGEEFVLILPMCNTLKVEQHLNGF
HHCHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
REKLASVVITNPADQVTFSVTVSIGAIIADAAYSSTLEVWLKQADENLYKAKEQGRNCVV
HHHHHHEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CTLVTV
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA