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The map label for this gene is mltB [H]

Identifier: 117920823

GI number: 117920823

Start: 2860378

End: 2861379

Strand: Reverse

Name: mltB [H]

Synonym: Shewana3_2380

Alternate gene names: 117920823

Gene position: 2861379-2860378 (Counterclockwise)

Preceding gene: 117920824

Following gene: 117920822

Centisome position: 57.55

GC content: 48.9

Gene sequence:

>1002_bases
ATGCCAAAGACCCTCCTTCAACGCGTTGCAGCATGTACATTGATTTTCTTGCCCTTGATAAGCCATAGCGCGCTAGGGCA
AGACGCCTCCTTCAACGATTACCTCCTTAAGCTTAAGCAAGAGGCGAGTGCCCAAGGCATTAGCCAAGCGACGATCAATA
CGGCTTTTCCGCAGATAAAACGCTTTAAAAAAGCCAATGTGGCCGCAGCGCCAAGCCAATCTAAAACCCTTGAAACCTAT
TTGCCAGAAGTGGTGCCGGAATGGAAGGTGGATACGGCGCGCGTACTCTTTAACGAGCATGAAGTGCTATTGAGCAATAT
TGCCGACAAGTATCAAGTGCAGCCGCGGTTTTTAGTGGCGCTCTGGGGCTTAGTGTCTAACTTTGGGGATGCGTCGGGGG
ATTATCCTGTACTGTCTGTGATGGCGTCGTTAGCCTTTACTGGCGAAGATACGGCGCGTTACCAACGGGAGTTTATGGCT
GCGCTTTCCATCATGGATAAAGAGCAACTTAAGTTTGATGATCTTAAAAGCAATGACAAAGGCTTTATGGGGCAAACCCA
ATTGCTACCGAGTGAATATTTAGCCTACGGCCAAGATGGCGACGGTGATGGTAAAAAAGACATTTGGCATAATGTGGCCG
ATGCGTTTGCCTCTGCCGCGAATCTGCTTAAGCAGCAGGGCTGGAATGGTGAAGATACTTGGGGACGTCAGGTTCAAGCA
CCGGCGGATATTAAGACCGAGTTTATCGGACTCAATCAATCACAGACCCTAGCCAAGTGGCAACAGCTCGGTGTCAGGCG
GTTCGATAATACGGATTTACCGAATCGTGAGGATATTCATGCTTCGCTTATCATGCCCGACGGTGTTAAGGGCCGAAAAT
ATCTGGTCTATGGCAATTACCGCGCCTTAATGCACTGGCAAGCGGTGAATTATTTAGGCGAGGGCGATTATTTTGGCATC
TCTGTGGTGCATTTATCGGAGAGAATTAAGACTCCCCAGTAA

Upstream 100 bases:

>100_bases
TTTTTATTTACAAATTCCGCCACCGCAGGTTAATTTATTGGCCGAACACAGAGAAAGCCTTGGGATTAAAGACTAGCCCA
TAAACTGCGGACAAAGGTAT

Downstream 100 bases:

>100_bases
ATTGGTGTAAAATGGCCCGCATTAAGCGGGTTTTTTATATGAAGATAAGCGTAAACATGGCATTTTGGCAGAATAAAGCG
TTAACCGAGTTGACGTCGCA

Product: putative transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MPKTLLQRVAACTLIFLPLISHSALGQDASFNDYLLKLKQEASAQGISQATINTAFPQIKRFKKANVAAAPSQSKTLETY
LPEVVPEWKVDTARVLFNEHEVLLSNIADKYQVQPRFLVALWGLVSNFGDASGDYPVLSVMASLAFTGEDTARYQREFMA
ALSIMDKEQLKFDDLKSNDKGFMGQTQLLPSEYLAYGQDGDGDGKKDIWHNVADAFASAANLLKQQGWNGEDTWGRQVQA
PADIKTEFIGLNQSQTLAKWQQLGVRRFDNTDLPNREDIHASLIMPDGVKGRKYLVYGNYRALMHWQAVNYLGEGDYFGI
SVVHLSERIKTPQ

Sequences:

>Translated_333_residues
MPKTLLQRVAACTLIFLPLISHSALGQDASFNDYLLKLKQEASAQGISQATINTAFPQIKRFKKANVAAAPSQSKTLETY
LPEVVPEWKVDTARVLFNEHEVLLSNIADKYQVQPRFLVALWGLVSNFGDASGDYPVLSVMASLAFTGEDTARYQREFMA
ALSIMDKEQLKFDDLKSNDKGFMGQTQLLPSEYLAYGQDGDGDGKKDIWHNVADAFASAANLLKQQGWNGEDTWGRQVQA
PADIKTEFIGLNQSQTLAKWQQLGVRRFDNTDLPNREDIHASLIMPDGVKGRKYLVYGNYRALMHWQAVNYLGEGDYFGI
SVVHLSERIKTPQ
>Mature_332_residues
PKTLLQRVAACTLIFLPLISHSALGQDASFNDYLLKLKQEASAQGISQATINTAFPQIKRFKKANVAAAPSQSKTLETYL
PEVVPEWKVDTARVLFNEHEVLLSNIADKYQVQPRFLVALWGLVSNFGDASGDYPVLSVMASLAFTGEDTARYQREFMAA
LSIMDKEQLKFDDLKSNDKGFMGQTQLLPSEYLAYGQDGDGDGKKDIWHNVADAFASAANLLKQQGWNGEDTWGRQVQAP
ADIKTEFIGLNQSQTLAKWQQLGVRRFDNTDLPNREDIHASLIMPDGVKGRKYLVYGNYRALMHWQAVNYLGEGDYFGIS
VVHLSERIKTPQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=199, Percent_Identity=30.1507537688442, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37244; Mature: 37112

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTLLQRVAACTLIFLPLISHSALGQDASFNDYLLKLKQEASAQGISQATINTAFPQIK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RFKKANVAAAPSQSKTLETYLPEVVPEWKVDTARVLFNEHEVLLSNIADKYQVQPRFLVA
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LWGLVSNFGDASGDYPVLSVMASLAFTGEDTARYQREFMAALSIMDKEQLKFDDLKSNDK
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCC
GFMGQTQLLPSEYLAYGQDGDGDGKKDIWHNVADAFASAANLLKQQGWNGEDTWGRQVQA
CCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PADIKTEFIGLNQSQTLAKWQQLGVRRFDNTDLPNREDIHASLIMPDGVKGRKYLVYGNY
CCCCHHHEECCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECH
RALMHWQAVNYLGEGDYFGISVVHLSERIKTPQ
HHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCC
>Mature Secondary Structure 
PKTLLQRVAACTLIFLPLISHSALGQDASFNDYLLKLKQEASAQGISQATINTAFPQIK
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RFKKANVAAAPSQSKTLETYLPEVVPEWKVDTARVLFNEHEVLLSNIADKYQVQPRFLVA
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LWGLVSNFGDASGDYPVLSVMASLAFTGEDTARYQREFMAALSIMDKEQLKFDDLKSNDK
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCC
GFMGQTQLLPSEYLAYGQDGDGDGKKDIWHNVADAFASAANLLKQQGWNGEDTWGRQVQA
CCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PADIKTEFIGLNQSQTLAKWQQLGVRRFDNTDLPNREDIHASLIMPDGVKGRKYLVYGNY
CCCCHHHEECCCCHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECH
RALMHWQAVNYLGEGDYFGISVVHLSERIKTPQ
HHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]