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The map label for this gene is nrfA [H]

Identifier: 117920806

GI number: 117920806

Start: 2840377

End: 2841810

Strand: Direct

Name: nrfA [H]

Synonym: Shewana3_2363

Alternate gene names: 117920806

Gene position: 2840377-2841810 (Clockwise)

Preceding gene: 117920805

Following gene: 117920809

Centisome position: 57.13

GC content: 50.42

Gene sequence:

>1434_bases
GTGAATTATTTTACCAAAACAACGGCCCTATCGGGTCTCAAGCTCAGTTTGCTCACCGCCGCGCTGCTGAGCAATTGGGG
CGCCATGGCCCAAAATGCACCGAATGAGTTACCAACAAGCGCAGCCCCAAAAAGCCATTTGCCAAAAGATGAAGTACAAA
ACAGCGATGCACAAAAGTTTCCGCTGCAACACCAGTCTTGGGGAGCAACCATTGAACAGGCCGAACGAGAAGACGTCCTT
GAGAAAAACCCGCGCAATGTCATTCTGTGGGCGGGCTCATCCTATGCTAAGGAATACCACAGTCCACGCGGACATCAGTT
TGCCGTCGCCGATGTGTCCCATACACTGCGCACAGGCGTAGCCCCCAAAGCGGGCGAAAAAGGCCTATCGGCCAGCTGCT
GGACCTGTAAAAGCCCAGACGCACCAAGGCTCATTGGCGAACTTGGCTTTGAAGGCTATGCCGCCAAAAACTTTACCGAC
CTTGGCGCCGAAATGAAAAGCGTGGTGTATTGCAGCGATTGCCACGAATCGGGCAGCGCAAAACTCACGCTTCCCCGCCC
CCATGCCAAGGATGCAATGGCAAAAATCCACCTTCCCTTCGAGCAACAAAATGCCACTATGCAGAGCGCTCAAGTTTGTG
GACAATGCCACGTTACCTACTATTTCCGCCCCGAGAAAAGTAACGTCGTGAATATTCCATGGATCTTCGGCAATACGGCC
GATGAGATTGAAAAATACTACGACACTCGCCGCTTCTATGAATGGGTCCATCCACTGTCGAAAGCCCCGATGCTTAAAGC
CCGCCACCCAGAATTTGAGCACTGGAGTCGCAGTAAACACGCTGAAGTGGGGGTCACCTGCGTCACCTGCCATATGCCAT
CGGCGCAGGATAAAAAAGGCAATGAATTTACTAATCACAAGGTAGACAAAGCCTTACCTAACTTTGAAACCAGTTGTAAA
GGTTGTCACAGCAGCAAGGACAAATTAGAAACCAAACTGACAGACTTAAAAACAGAAATTGATAACAAGGCCCATGCGGT
TGAAGATCTGCTGGTTAAAGCCCACTACGAAGCCAAGGCGGCTTGGGTTGCAGGCGCCACATGGGAGCAAATGAATGACC
CTATTATGGATATCCGTCATAGCCAATGGCGCTGGGATTTTGCCATGTCATCCCACGGCCTTTACGCCCATAACCCGAAG
GAAGGCCGCCAACTACTCGATACCGCCTTAGAGCAAGCCAAGAGTGCGCGCACAAGCCTAGCCGAGGTACTAAAGCAATT
AAACAGTGAGGTATACTACCCTGATATCAGTACAAAAGCGAAGGCGCAGGAAGCAATTGGTCTCAACGAAGCCGAACTAA
TCAAGGAAAAACAGCAGTTTATTAAGGAAGAAATCGATAAAAACTGGGATCCAGTCGCCCACAGAGGTTATTGA

Upstream 100 bases:

>100_bases
TAGTGCATTCGCCCTGTTTGTCTGGCACTACAGCCCTATTCTCACTCGCCCACGCATCGATGGACGCCCAGGTTAACTTT
CAAATTATAGGAAAATAAAC

Downstream 100 bases:

>100_bases
TCCGATCAGCCTAGAAGGGATTTATTAATGTGTGTAATTCATAGTGATCTGATCCAGTATTCATAGTGAGAAGATCCGTC
CGTTGTGTTTGGGACTCAAG

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNYFTKTTALSGLKLSLLTAALLSNWGAMAQNAPNELPTSAAPKSHLPKDEVQNSDAQKFPLQHQSWGATIEQAEREDVL
EKNPRNVILWAGSSYAKEYHSPRGHQFAVADVSHTLRTGVAPKAGEKGLSASCWTCKSPDAPRLIGELGFEGYAAKNFTD
LGAEMKSVVYCSDCHESGSAKLTLPRPHAKDAMAKIHLPFEQQNATMQSAQVCGQCHVTYYFRPEKSNVVNIPWIFGNTA
DEIEKYYDTRRFYEWVHPLSKAPMLKARHPEFEHWSRSKHAEVGVTCVTCHMPSAQDKKGNEFTNHKVDKALPNFETSCK
GCHSSKDKLETKLTDLKTEIDNKAHAVEDLLVKAHYEAKAAWVAGATWEQMNDPIMDIRHSQWRWDFAMSSHGLYAHNPK
EGRQLLDTALEQAKSARTSLAEVLKQLNSEVYYPDISTKAKAQEAIGLNEAELIKEKQQFIKEEIDKNWDPVAHRGY

Sequences:

>Translated_477_residues
MNYFTKTTALSGLKLSLLTAALLSNWGAMAQNAPNELPTSAAPKSHLPKDEVQNSDAQKFPLQHQSWGATIEQAEREDVL
EKNPRNVILWAGSSYAKEYHSPRGHQFAVADVSHTLRTGVAPKAGEKGLSASCWTCKSPDAPRLIGELGFEGYAAKNFTD
LGAEMKSVVYCSDCHESGSAKLTLPRPHAKDAMAKIHLPFEQQNATMQSAQVCGQCHVTYYFRPEKSNVVNIPWIFGNTA
DEIEKYYDTRRFYEWVHPLSKAPMLKARHPEFEHWSRSKHAEVGVTCVTCHMPSAQDKKGNEFTNHKVDKALPNFETSCK
GCHSSKDKLETKLTDLKTEIDNKAHAVEDLLVKAHYEAKAAWVAGATWEQMNDPIMDIRHSQWRWDFAMSSHGLYAHNPK
EGRQLLDTALEQAKSARTSLAEVLKQLNSEVYYPDISTKAKAQEAIGLNEAELIKEKQQFIKEEIDKNWDPVAHRGY
>Mature_477_residues
MNYFTKTTALSGLKLSLLTAALLSNWGAMAQNAPNELPTSAAPKSHLPKDEVQNSDAQKFPLQHQSWGATIEQAEREDVL
EKNPRNVILWAGSSYAKEYHSPRGHQFAVADVSHTLRTGVAPKAGEKGLSASCWTCKSPDAPRLIGELGFEGYAAKNFTD
LGAEMKSVVYCSDCHESGSAKLTLPRPHAKDAMAKIHLPFEQQNATMQSAQVCGQCHVTYYFRPEKSNVVNIPWIFGNTA
DEIEKYYDTRRFYEWVHPLSKAPMLKARHPEFEHWSRSKHAEVGVTCVTCHMPSAQDKKGNEFTNHKVDKALPNFETSCK
GCHSSKDKLETKLTDLKTEIDNKAHAVEDLLVKAHYEAKAAWVAGATWEQMNDPIMDIRHSQWRWDFAMSSHGLYAHNPK
EGRQLLDTALEQAKSARTSLAEVLKQLNSEVYYPDISTKAKAQEAIGLNEAELIKEKQQFIKEEIDKNWDPVAHRGY

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=437, Percent_Identity=45.9954233409611, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 53551; Mature: 53551

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYFTKTTALSGLKLSLLTAALLSNWGAMAQNAPNELPTSAAPKSHLPKDEVQNSDAQKF
CCCCCCHHHHCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCHHC
PLQHQSWGATIEQAEREDVLEKNPRNVILWAGSSYAKEYHSPRGHQFAVADVSHTLRTGV
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCEEEEHHHHHHHHHCC
APKAGEKGLSASCWTCKSPDAPRLIGELGFEGYAAKNFTDLGAEMKSVVYCSDCHESGSA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
KLTLPRPHAKDAMAKIHLPFEQQNATMQSAQVCGQCHVTYYFRPEKSNVVNIPWIFGNTA
EEEECCCCHHHHHEEEECCHHHHCCHHHHHHHCCCEEEEEEEECCCCCEEEECEEECCCH
DEIEKYYDTRRFYEWVHPLSKAPMLKARHPEFEHWSRSKHAEVGVTCVTCHMPSAQDKKG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCC
NEFTNHKVDKALPNFETSCKGCHSSKDKLETKLTDLKTEIDNKAHAVEDLLVKAHYEAKA
CCCCCCHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHE
AWVAGATWEQMNDPIMDIRHSQWRWDFAMSSHGLYAHNPKEGRQLLDTALEQAKSARTSL
HEEECCCHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEVLKQLNSEVYYPDISTKAKAQEAIGLNEAELIKEKQQFIKEEIDKNWDPVAHRGY
HHHHHHHCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNYFTKTTALSGLKLSLLTAALLSNWGAMAQNAPNELPTSAAPKSHLPKDEVQNSDAQKF
CCCCCCHHHHCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCHHC
PLQHQSWGATIEQAEREDVLEKNPRNVILWAGSSYAKEYHSPRGHQFAVADVSHTLRTGV
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCEEEEHHHHHHHHHCC
APKAGEKGLSASCWTCKSPDAPRLIGELGFEGYAAKNFTDLGAEMKSVVYCSDCHESGSA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
KLTLPRPHAKDAMAKIHLPFEQQNATMQSAQVCGQCHVTYYFRPEKSNVVNIPWIFGNTA
EEEECCCCHHHHHEEEECCHHHHCCHHHHHHHCCCEEEEEEEECCCCCEEEECEEECCCH
DEIEKYYDTRRFYEWVHPLSKAPMLKARHPEFEHWSRSKHAEVGVTCVTCHMPSAQDKKG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCC
NEFTNHKVDKALPNFETSCKGCHSSKDKLETKLTDLKTEIDNKAHAVEDLLVKAHYEAKA
CCCCCCHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHE
AWVAGATWEQMNDPIMDIRHSQWRWDFAMSSHGLYAHNPKEGRQLLDTALEQAKSARTSL
HEEECCCHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEVLKQLNSEVYYPDISTKAKAQEAIGLNEAELIKEKQQFIKEEIDKNWDPVAHRGY
HHHHHHHCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA