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The map label for this gene is trmJ [H]

Identifier: 117920726

GI number: 117920726

Start: 2745028

End: 2745753

Strand: Reverse

Name: trmJ [H]

Synonym: Shewana3_2283

Alternate gene names: 117920726

Gene position: 2745753-2745028 (Counterclockwise)

Preceding gene: 117920728

Following gene: 117920725

Centisome position: 55.22

GC content: 47.52

Gene sequence:

>726_bases
ATGCTAAGTAATATTCGTGTGATTTTAGTGGGGACATCGCACCCCGGCAATATTGGTTCGACCGCCAGAGCGATGAAGAC
CATGGGGTTATCGACCCTGTATCTTGCCGAACCAAGATGTGAAGTGGATGGTCAATCCATTGCCTTGGCCGCTGGTGCAT
CGGACATTTTAAAACATTTGGTGAAAGTGGATTCCTTAGAGGAAGCCATCGCCGATTGCAGTTTAGTAATTGCCACCAGT
GCCCGCAGCCGTACCTTAGATTGGCCAATGCTCGACCCGCGTGAAGCGGGGCAAAAGCTGGTCACTGAGGGCTTAACAGG
GCCTGTCGCCATTGTCTTTGGCCGTGAAAATCATGGTTTGACCAATGAAGAATTACAGCAATGTACATATCATGTGGCTA
TTCCTGCTAACCCTGAATACAGCTCATTGAATTTAGCGCAAGCAGTGCAGATCATTTGTTATGAGACGCGTGTGGCACAC
TTAGCGCAAACTGAGGCGCCTCAAGTGGAGGAAGAGTATCCGCTTTCTGCCGATCTCGAGCGTTTCTTTGTGCACTTAGA
AAGCACCTTATCATCGACAGGTTTTATTATTAAAAACCACCCCGGCCAAGTGATGACTAAGCTACGTCGTCTGTTTAGCC
GTGCACGTATCGAAAGCCAAGAGATGAACATTCTTCGAGGCATTTTAACCTCGATAGATAAAGTGGTGGGTAACAAACAA
AAATAG

Upstream 100 bases:

>100_bases
ATTTGAATGTTATATCAAATACCGATTTTCATATAAGCATTCACGCACGCCTGTGATAGTATTTGCTCCCTGTGTTTTGT
GAATTAAATTAAGTCGTTTC

Downstream 100 bases:

>100_bases
CCAAAGCACATTTGGCAAATAACGCTAATCGCGAAGGATTGAAGCATGGGTGTGATAGCTAGGCTAAAAGAAGACATTGA
ATCTATATATCACCGTGATC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLSNIRVILVGTSHPGNIGSTARAMKTMGLSTLYLAEPRCEVDGQSIALAAGASDILKHLVKVDSLEEAIADCSLVIATS
ARSRTLDWPMLDPREAGQKLVTEGLTGPVAIVFGRENHGLTNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQTEAPQVEEEYPLSADLERFFVHLESTLSSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVGNKQ
K

Sequences:

>Translated_241_residues
MLSNIRVILVGTSHPGNIGSTARAMKTMGLSTLYLAEPRCEVDGQSIALAAGASDILKHLVKVDSLEEAIADCSLVIATS
ARSRTLDWPMLDPREAGQKLVTEGLTGPVAIVFGRENHGLTNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQTEAPQVEEEYPLSADLERFFVHLESTLSSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVGNKQ
K
>Mature_241_residues
MLSNIRVILVGTSHPGNIGSTARAMKTMGLSTLYLAEPRCEVDGQSIALAAGASDILKHLVKVDSLEEAIADCSLVIATS
ARSRTLDWPMLDPREAGQKLVTEGLTGPVAIVFGRENHGLTNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAQTEAPQVEEEYPLSADLERFFVHLESTLSSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVGNKQ
K

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=67.2131147540984, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1790865, Length=152, Percent_Identity=37.5, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26394; Mature: 26394

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVILVGTSHPGNIGSTARAMKTMGLSTLYLAEPRCEVDGQSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLEEAIADCSLVIATSARSRTLDWPMLDPREAGQKLVTEGLTGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
TNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAQTEAPQVEEEYPLSADLE
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RFFVHLESTLSSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVGNKQ
HHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
K
C
>Mature Secondary Structure
MLSNIRVILVGTSHPGNIGSTARAMKTMGLSTLYLAEPRCEVDGQSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLEEAIADCSLVIATSARSRTLDWPMLDPREAGQKLVTEGLTGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
TNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAQTEAPQVEEEYPLSADLE
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RFFVHLESTLSSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVGNKQ
HHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA