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The map label for this gene is ptsI [H]

Identifier: 117920700

GI number: 117920700

Start: 2718641

End: 2720344

Strand: Direct

Name: ptsI [H]

Synonym: Shewana3_2257

Alternate gene names: 117920700

Gene position: 2718641-2720344 (Clockwise)

Preceding gene: 117920699

Following gene: 117920701

Centisome position: 54.68

GC content: 52.29

Gene sequence:

>1704_bases
ATGTCGATTACGGGGATCATAGTGTCATCGGGGATTGCCTTCGGACAGGCGCTCCACCTTACCCAGGCCGAACACCACCT
CGATTATCGCCCTATTCCCCTATCACGGATCCCGCAGCAACAGAGTAAATTTGTTAAAGCCCTACAGGCACTGCAGCAAC
AACTCAGCCACAGCCAAACTAAGCTCGACCCACAAAGCGAAAACTATCAGTTAATTGAAGCCGATTTGTTACTGCTTGAG
GACGAAGAGCTTATCGACCAAGTGAAGGAAGCGATCCGCACCCTGCAGTTATCTGCCAGTGTAGCGGTTGAGCGGATCTT
CGCCCATCAGGCGAATGAGCTTGAATCCTTAGACGATCCCTATCTTGCCAATCGTGCCCAAGATGTACGATGCCTAGGCC
AGCGATTAGTCACGGCTATTAACGGTCGCTTAGATCAAGGCTTAGCCCAGTTAACTGAGCCGACGATCCTCCTGGCGCAA
GATCTCACTCCCGCCGAATTTGCGTTATTACCTAAAGAGCAGCTCAGCGGCATAGTACTTAAAACCGGCGGCTTAACCAG
CCATACGGCGATTTTAGCCCGCGCGGCGGGGATCCCCGCTATTCTTAGCTGCCAGTTCGATGCCGAGTTTATTCCCAATG
GCACACCACTGGTGCTCGATGCCCTCAGTGGTGAGCTTTTTGTTAACCCAGCACCCGAGCTACAGGCACGCTTAACCGTC
ACATTGCACCATGAGCAAGCCAGACGCGCCGCCCTGCAAGCGTACCGAGATGTGCCCGCCAAGACCCAAGATGGCCACCT
CGTAGGCCTGATGGCCAACGTGGGCAACTTGAACGATATCACCCATGTTGGCGATGTGGGTGCCGATGGTATTGGTCTGT
TTCGCACCGAGTTTATGCTGATGCACACCAGTACACTGCCGGATGAAAAAGCGCAATACAATCTATACTGCGAAGCCTTG
CATGCGTTAGGTGGTAAAACCTTTACGATCAGAACCTTAGATATTGGTGCCGATAAAGAGCTGCCCTGCCTCTGCCAGGA
GGTCGAGGACAATCCCGCCCTTGGGCTACGCGGAGTTAGATATACCTTGGCGCATCCTGAGTTGTTTAAGACCCAGCTTA
GGGCGATTTTGCGAGCAGCCAACCATGGCCCCATTCGACTCATGTTTCCCATGGTGAATCAGGTTGAAGAACTCGATCAG
ATTTTTGCCCTCATCGCCGAATGCCAAGACGCCCTCGAAGAGGAAGAAAAAGGCTTCGGCGAACTCAGTTACGGCATAGT
GGTTGAAACCCCCGCCGCCGTGATGAATCTTGCTTCTATGCTGCCAAGATTAGATTTTGTGAGCATAGGCACCAATGACT
TAACCCAATATGCCATGGCGGCGGATCGCACAAATCCACAACTGACACGGGATTATCCTTCCCTGTCACCGGCGATTTTA
GGGCTCATCAAGATGACCGTCGACCAAGCCAAAGCCGCTGGAGTCAAAGTGTCACTCTGTGGCGAACTTGCCAGCTCGCC
GCTGATGGTCCCGCTCCTTATCGGTATGGGACTCGATGAACTCAGCGTAAACTTAAGCGCATTGCTGGAGGTAAAGGCCG
CCGTTTGCCAAGGGCAATTGACGAAATTTTCTGCGTTAGCGCACACCGCCATGCAACAGAATAGAATTTCAGAACTACAG
CAGTGTATAACAAGCTATAAATAA

Upstream 100 bases:

>100_bases
TTGCCGAAGGTGAGCAGGCACAGGAAGCGGTCGAGAAAGTCTCGGAACTGCTCATCACTTTAAGTTAAACGGCATCTTAG
CCAGCTTGATAGGAACGAAT

Downstream 100 bases:

>100_bases
TACATTTTATTGGTTATGCTGCTTTACAGACTAAAAAGATAACAACAAAAACAAGCGAAGGGGCACAGGATAATGGGATT
TTTAAGCCGAATAAGGCGAT

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSITGIIVSSGIAFGQALHLTQAEHHLDYRPIPLSRIPQQQSKFVKALQALQQQLSHSQTKLDPQSENYQLIEADLLLLE
DEELIDQVKEAIRTLQLSASVAVERIFAHQANELESLDDPYLANRAQDVRCLGQRLVTAINGRLDQGLAQLTEPTILLAQ
DLTPAEFALLPKEQLSGIVLKTGGLTSHTAILARAAGIPAILSCQFDAEFIPNGTPLVLDALSGELFVNPAPELQARLTV
TLHHEQARRAALQAYRDVPAKTQDGHLVGLMANVGNLNDITHVGDVGADGIGLFRTEFMLMHTSTLPDEKAQYNLYCEAL
HALGGKTFTIRTLDIGADKELPCLCQEVEDNPALGLRGVRYTLAHPELFKTQLRAILRAANHGPIRLMFPMVNQVEELDQ
IFALIAECQDALEEEEKGFGELSYGIVVETPAAVMNLASMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
GLIKMTVDQAKAAGVKVSLCGELASSPLMVPLLIGMGLDELSVNLSALLEVKAAVCQGQLTKFSALAHTAMQQNRISELQ
QCITSYK

Sequences:

>Translated_567_residues
MSITGIIVSSGIAFGQALHLTQAEHHLDYRPIPLSRIPQQQSKFVKALQALQQQLSHSQTKLDPQSENYQLIEADLLLLE
DEELIDQVKEAIRTLQLSASVAVERIFAHQANELESLDDPYLANRAQDVRCLGQRLVTAINGRLDQGLAQLTEPTILLAQ
DLTPAEFALLPKEQLSGIVLKTGGLTSHTAILARAAGIPAILSCQFDAEFIPNGTPLVLDALSGELFVNPAPELQARLTV
TLHHEQARRAALQAYRDVPAKTQDGHLVGLMANVGNLNDITHVGDVGADGIGLFRTEFMLMHTSTLPDEKAQYNLYCEAL
HALGGKTFTIRTLDIGADKELPCLCQEVEDNPALGLRGVRYTLAHPELFKTQLRAILRAANHGPIRLMFPMVNQVEELDQ
IFALIAECQDALEEEEKGFGELSYGIVVETPAAVMNLASMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
GLIKMTVDQAKAAGVKVSLCGELASSPLMVPLLIGMGLDELSVNLSALLEVKAAVCQGQLTKFSALAHTAMQQNRISELQ
QCITSYK
>Mature_566_residues
SITGIIVSSGIAFGQALHLTQAEHHLDYRPIPLSRIPQQQSKFVKALQALQQQLSHSQTKLDPQSENYQLIEADLLLLED
EELIDQVKEAIRTLQLSASVAVERIFAHQANELESLDDPYLANRAQDVRCLGQRLVTAINGRLDQGLAQLTEPTILLAQD
LTPAEFALLPKEQLSGIVLKTGGLTSHTAILARAAGIPAILSCQFDAEFIPNGTPLVLDALSGELFVNPAPELQARLTVT
LHHEQARRAALQAYRDVPAKTQDGHLVGLMANVGNLNDITHVGDVGADGIGLFRTEFMLMHTSTLPDEKAQYNLYCEALH
ALGGKTFTIRTLDIGADKELPCLCQEVEDNPALGLRGVRYTLAHPELFKTQLRAILRAANHGPIRLMFPMVNQVEELDQI
FALIAECQDALEEEEKGFGELSYGIVVETPAAVMNLASMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAILG
LIKMTVDQAKAAGVKVSLCGELASSPLMVPLLIGMGLDELSVNLSALLEVKAAVCQGQLTKFSALAHTAMQQNRISELQQ
CITSYK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=567, Percent_Identity=37.2134038800705, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789193, Length=473, Percent_Identity=35.0951374207188, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=33.5676625659051, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI48994992, Length=510, Percent_Identity=33.7254901960784, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1787994, Length=420, Percent_Identity=25.4761904761905, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61722; Mature: 61591

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITGIIVSSGIAFGQALHLTQAEHHLDYRPIPLSRIPQQQSKFVKALQALQQQLSHSQT
CCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
KLDPQSENYQLIEADLLLLEDEELIDQVKEAIRTLQLSASVAVERIFAHQANELESLDDP
CCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRLVTAINGRLDQGLAQLTEPTILLAQDLTPAEFALLPKEQLSGIVL
HHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHHHEECCHHHHCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDAEFIPNGTPLVLDALSGELFVNPAPELQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEEE
TLHHEQARRAALQAYRDVPAKTQDGHLVGLMANVGNLNDITHVGDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHE
MHTSTLPDEKAQYNLYCEALHALGGKTFTIRTLDIGADKELPCLCQEVEDNPALGLRGVR
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCE
YTLAHPELFKTQLRAILRAANHGPIRLMFPMVNQVEELDQIFALIAECQDALEEEEKGFG
EEECCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVMNLASMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
CEECCEEEECHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
GLIKMTVDQAKAAGVKVSLCGELASSPLMVPLLIGMGLDELSVNLSALLEVKAAVCQGQL
HHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TKFSALAHTAMQQNRISELQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITGIIVSSGIAFGQALHLTQAEHHLDYRPIPLSRIPQQQSKFVKALQALQQQLSHSQT
CEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
KLDPQSENYQLIEADLLLLEDEELIDQVKEAIRTLQLSASVAVERIFAHQANELESLDDP
CCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRLVTAINGRLDQGLAQLTEPTILLAQDLTPAEFALLPKEQLSGIVL
HHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHHHEECCHHHHCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDAEFIPNGTPLVLDALSGELFVNPAPELQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCEEEEEE
TLHHEQARRAALQAYRDVPAKTQDGHLVGLMANVGNLNDITHVGDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHE
MHTSTLPDEKAQYNLYCEALHALGGKTFTIRTLDIGADKELPCLCQEVEDNPALGLRGVR
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCE
YTLAHPELFKTQLRAILRAANHGPIRLMFPMVNQVEELDQIFALIAECQDALEEEEKGFG
EEECCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVMNLASMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
CEECCEEEECHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
GLIKMTVDQAKAAGVKVSLCGELASSPLMVPLLIGMGLDELSVNLSALLEVKAAVCQGQL
HHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TKFSALAHTAMQQNRISELQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]