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The map label for this gene is ribE [H]

Identifier: 117920485

GI number: 117920485

Start: 2449582

End: 2450202

Strand: Direct

Name: ribE [H]

Synonym: Shewana3_2040

Alternate gene names: 117920485

Gene position: 2449582-2450202 (Clockwise)

Preceding gene: 117920483

Following gene: 117920487

Centisome position: 49.27

GC content: 47.34

Gene sequence:

>621_bases
ATGTTTACTGGTATAGTTCAAGCCACCTGTGAGGTGGTTGCAATACATAAGAAAGATGGCCTAAATACCCTTGAGGTAGC
GTTTAAACCTGATTTGCATGAGGGACTTGCGATTGGCGCAAGTGTGGCAAATAACGGGGTATGTCTGACGGTGACTCAGG
TCGTTGATGATAGGGTATTTTTCGATGTAGTGGAAGAGACCTTAAGGTTGACAAACCTTGCCAATTTGACCGTTGGTCAG
CGGGTGAACATTGAGCGTTCTTTGACGTTCGGTAGTGAAATCGGCGGCCATATCCTCTCGGGACACATTCATACCAAGGC
GAAAGTTAGCCACATTAGTCACACCGAGCAGCATTATGATCTGACCTTAAGCGTCGAGCCCAAATGGATGAACTATATCC
TTTATAAAGGCTTTGTCGGGGTAAACGGTTGCAGCTTAACGGTCGGCGAGGTGACCGAGAGCAGTTTTATGCTGCACTTG
ATCCCAGAAACCCTCAAATTGACCAACTTAGGCCAGTGTCAGGTTGGAGATGAGCTAAATATCGAAATTGACAGCCAAAC
CCAAGCCATCGTCGATACCGTCGAGCGTGTGCTGGCCAAACGTTTTAGCGGCCAATCTTAA

Upstream 100 bases:

>100_bases
CTTGGGCAATCAACACAGGGAGTGCGAGTTGAATTAAGCGTTTGGCCTGAAAGCCTGAATGATTCATAAATACAGCCTTT
AACAAATAAGCGAGCAAAAA

Downstream 100 bases:

>100_bases
GCTACATGCCTAGTAAATTTGCTCGTCGTCCGAGTCAATATATAACTCTATGGTGCGGCGGGCATCATCCACATGTAAAC
GCAGGTGGATGGGATTACAA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLTVGQ
RVNIERSLTFGSEIGGHILSGHIHTKAKVSHISHTEQHYDLTLSVEPKWMNYILYKGFVGVNGCSLTVGEVTESSFMLHL
IPETLKLTNLGQCQVGDELNIEIDSQTQAIVDTVERVLAKRFSGQS

Sequences:

>Translated_206_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLTVGQ
RVNIERSLTFGSEIGGHILSGHIHTKAKVSHISHTEQHYDLTLSVEPKWMNYILYKGFVGVNGCSLTVGEVTESSFMLHL
IPETLKLTNLGQCQVGDELNIEIDSQTQAIVDTVERVLAKRFSGQS
>Mature_206_residues
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVFFDVVEETLRLTNLANLTVGQ
RVNIERSLTFGSEIGGHILSGHIHTKAKVSHISHTEQHYDLTLSVEPKWMNYILYKGFVGVNGCSLTVGEVTESSFMLHL
IPETLKLTNLGQCQVGDELNIEIDSQTQAIVDTVERVLAKRFSGQS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=53.960396039604, Blast_Score=212, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319733, Length=197, Percent_Identity=29.4416243654822, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22533; Mature: 22533

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVF
CCCCHHCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHCCHHH
FDVVEETLRLTNLANLTVGQRVNIERSLTFGSEIGGHILSGHIHTKAKVSHISHTEQHYD
HHHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCCCEEEECEEECCCHHHHHHCCCCCEE
LTLSVEPKWMNYILYKGFVGVNGCSLTVGEVTESSFMLHLIPETLKLTNLGQCQVGDELN
EEEEECCHHHEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCHHHHCCCCCEECCCEEE
IEIDSQTQAIVDTVERVLAKRFSGQS
EEECCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVQATCEVVAIHKKDGLNTLEVAFKPDLHEGLAIGASVANNGVCLTVTQVVDDRVF
CCCCHHCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHCCHHH
FDVVEETLRLTNLANLTVGQRVNIERSLTFGSEIGGHILSGHIHTKAKVSHISHTEQHYD
HHHHHHHHHHHHHCCCCCCCEECCEEEEECCCCCCCEEEECEEECCCHHHHHHCCCCCEE
LTLSVEPKWMNYILYKGFVGVNGCSLTVGEVTESSFMLHLIPETLKLTNLGQCQVGDELN
EEEEECCHHHEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCHHHHCCCCCEECCCEEE
IEIDSQTQAIVDTVERVLAKRFSGQS
EEECCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]