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The map label for this gene is oprM [H]

Identifier: 117920439

GI number: 117920439

Start: 2376345

End: 2377832

Strand: Direct

Name: oprM [H]

Synonym: Shewana3_1994

Alternate gene names: 117920439

Gene position: 2376345-2377832 (Clockwise)

Preceding gene: 117920438

Following gene: 117920442

Centisome position: 47.79

GC content: 49.06

Gene sequence:

>1488_bases
GTGGTTAAGCATCATACTCTCTCCAGCCATCCATCGTCGGCGACTCGGCCTGTGATGCAGTCTGTTTCAGTGGGTTTGCG
TTTAAAGCCGCTTTATTTGGGACTCGTGACGGCATTGAGTGTTGCAGGCTGCGCCATGGGGCCCGATTACCAGCGGCCAG
AGCTAGTGTTACCGAATCAGTATCAAGCGCAAATGTTGTCGCAAACCACTGCTGAGCAGGGGAATGTAAAGGAAGTCTCG
GCCCTATCATGGCGGCATTTTTATCAAGACCCTGTGCTGATTTCCTTGATTGAGCATGCGCTGGCGAACAATCTGGATTT
GCAAATGGCGCAGTCGCGCCTTCTCGCTGCACGTTCAAAAATGACAGTGGTCGACAGTGCGCTTTGGCCTGAGGTTTCAG
TCAAAGCGGGATATGAACGCAGTTTAGATAGCGGTGCAACCAGCACTAATCCAAGTCCGAGTACCACCTTAGATTTAGGC
GGCGCGGTTTCGTGGGAGCTAGACCTTTGGGGCGCAAACCGCAGGGCGAGCGAGGCTGCTATGGCTGATTATCTTTCGGA
GGTTGAAAAGCTCAGGCTCACCTATGTCAGCCTTATCAGTGATATTGCTAGCCGTTATTACGAATGGCTCGATATCGAGC
AGCGTTATAGTGTGTCTATCGACACCGTGGGATTACGGCAGAAGGAACTGGATATCGCCAAGCTTCGCCATGCTAATGGC
GTGATTTCGGGCCTCGATGTGCGTCAGGCAGAAGTTGAGCTTCAAAGCACCAAAGTGACCTTGCCGACATTAGACTACGA
AAGAAAATATAAAATAAATCAGTTGCATATCTTACTCGGTGAATATGATTATGCTTTACCATCTCCCCAAGGCTTACCCG
AAAATATGGGTTTACCCTATGAACTCAACACAGGTGTTCCTTCTGAATTGTTGACATTAAGACCCGATGTCAAAATTGCC
GAGCAGCAGATGATTGCAGCGAATGCGGAAGTGGGTATCGCCAAGGCGGCCTTTTTCCCTAAATTTACCATTTCGGGTGT
GTATGGTCGGGAGAATGATCATCTTAAAGATATCTTCGACAGTAATGGTGTCACTTGGTCACTGCTTGGCGGCATTAGTG
CACCGATTTTTAACCGCGGAAAGATTGCTGCTGAATATGATATCGCCACCGAAGCCGCGAAACAGTCGATGTTAAGTTAC
CGCAATGTGGTGCTGACCGCTTACTTTGATGTGAACGATGCGCTGAATAATCTTAAACGTGCCCAAGAGGCCATCAAAGC
GCAAAAGGAATTAGTTGAATCTTCATCGGCTTATGCACGTCTTGCACGACTGCGTTACCAAAATGGTGTTGCCACTTCGC
TGGATTTAATGGATGCGCAGCGGCAATTGTTTAGCGCCCAATTGGCCTACAGCGAGATCTTAAGGGATAAGCAATTGGCC
AAAATCGCCCTGTATCGAGCCCTCGGTGGTGGTGCAGTCAGTGAATAA

Upstream 100 bases:

>100_bases
GTGACGACCGCAGGTTGGGTAAAAAAGAAAACCATTAAACAACCTATTAAAACAAAAGAGCAATTACTCCTAGATCAAGA
AACCGTTGAGGAGGTGAGCC

Downstream 100 bases:

>100_bases
AAAATCCCCGCATGGCGGGGATTTTTATCGGGTTTAGGTTGGGTGACTGATTAGACCGCCACTTCTTTACCATCGAGTTT
ACGGATCACCACAGTTGCCG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MVKHHTLSSHPSSATRPVMQSVSVGLRLKPLYLGLVTALSVAGCAMGPDYQRPELVLPNQYQAQMLSQTTAEQGNVKEVS
ALSWRHFYQDPVLISLIEHALANNLDLQMAQSRLLAARSKMTVVDSALWPEVSVKAGYERSLDSGATSTNPSPSTTLDLG
GAVSWELDLWGANRRASEAAMADYLSEVEKLRLTYVSLISDIASRYYEWLDIEQRYSVSIDTVGLRQKELDIAKLRHANG
VISGLDVRQAEVELQSTKVTLPTLDYERKYKINQLHILLGEYDYALPSPQGLPENMGLPYELNTGVPSELLTLRPDVKIA
EQQMIAANAEVGIAKAAFFPKFTISGVYGRENDHLKDIFDSNGVTWSLLGGISAPIFNRGKIAAEYDIATEAAKQSMLSY
RNVVLTAYFDVNDALNNLKRAQEAIKAQKELVESSSAYARLARLRYQNGVATSLDLMDAQRQLFSAQLAYSEILRDKQLA
KIALYRALGGGAVSE

Sequences:

>Translated_495_residues
MVKHHTLSSHPSSATRPVMQSVSVGLRLKPLYLGLVTALSVAGCAMGPDYQRPELVLPNQYQAQMLSQTTAEQGNVKEVS
ALSWRHFYQDPVLISLIEHALANNLDLQMAQSRLLAARSKMTVVDSALWPEVSVKAGYERSLDSGATSTNPSPSTTLDLG
GAVSWELDLWGANRRASEAAMADYLSEVEKLRLTYVSLISDIASRYYEWLDIEQRYSVSIDTVGLRQKELDIAKLRHANG
VISGLDVRQAEVELQSTKVTLPTLDYERKYKINQLHILLGEYDYALPSPQGLPENMGLPYELNTGVPSELLTLRPDVKIA
EQQMIAANAEVGIAKAAFFPKFTISGVYGRENDHLKDIFDSNGVTWSLLGGISAPIFNRGKIAAEYDIATEAAKQSMLSY
RNVVLTAYFDVNDALNNLKRAQEAIKAQKELVESSSAYARLARLRYQNGVATSLDLMDAQRQLFSAQLAYSEILRDKQLA
KIALYRALGGGAVSE
>Mature_495_residues
MVKHHTLSSHPSSATRPVMQSVSVGLRLKPLYLGLVTALSVAGCAMGPDYQRPELVLPNQYQAQMLSQTTAEQGNVKEVS
ALSWRHFYQDPVLISLIEHALANNLDLQMAQSRLLAARSKMTVVDSALWPEVSVKAGYERSLDSGATSTNPSPSTTLDLG
GAVSWELDLWGANRRASEAAMADYLSEVEKLRLTYVSLISDIASRYYEWLDIEQRYSVSIDTVGLRQKELDIAKLRHANG
VISGLDVRQAEVELQSTKVTLPTLDYERKYKINQLHILLGEYDYALPSPQGLPENMGLPYELNTGVPSELLTLRPDVKIA
EQQMIAANAEVGIAKAAFFPKFTISGVYGRENDHLKDIFDSNGVTWSLLGGISAPIFNRGKIAAEYDIATEAAKQSMLSY
RNVVLTAYFDVNDALNNLKRAQEAIKAQKELVESSSAYARLARLRYQNGVATSLDLMDAQRQLFSAQLAYSEILRDKQLA
KIALYRALGGGAVSE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=30.4347826086957, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI2367348, Length=434, Percent_Identity=24.8847926267281, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54594; Mature: 54594

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKHHTLSSHPSSATRPVMQSVSVGLRLKPLYLGLVTALSVAGCAMGPDYQRPELVLPNQ
CCCCCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
YQAQMLSQTTAEQGNVKEVSALSWRHFYQDPVLISLIEHALANNLDLQMAQSRLLAARSK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MTVVDSALWPEVSVKAGYERSLDSGATSTNPSPSTTLDLGGAVSWELDLWGANRRASEAA
HHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCHHHHH
MADYLSEVEKLRLTYVSLISDIASRYYEWLDIEQRYSVSIDTVGLRQKELDIAKLRHANG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
VISGLDVRQAEVELQSTKVTLPTLDYERKYKINQLHILLGEYDYALPSPQGLPENMGLPY
CEECCCCHHHHEEEECCEEEECCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCE
ELNTGVPSELLTLRPDVKIAEQQMIAANAEVGIAKAAFFPKFTISGVYGRENDHLKDIFD
EECCCCCHHHHEECCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHC
SNGVTWSLLGGISAPIFNRGKIAAEYDIATEAAKQSMLSYRNVVLTAYFDVNDALNNLKR
CCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
AQEAIKAQKELVESSSAYARLARLRYQNGVATSLDLMDAQRQLFSAQLAYSEILRDKQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIALYRALGGGAVSE
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MVKHHTLSSHPSSATRPVMQSVSVGLRLKPLYLGLVTALSVAGCAMGPDYQRPELVLPNQ
CCCCCCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCH
YQAQMLSQTTAEQGNVKEVSALSWRHFYQDPVLISLIEHALANNLDLQMAQSRLLAARSK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MTVVDSALWPEVSVKAGYERSLDSGATSTNPSPSTTLDLGGAVSWELDLWGANRRASEAA
HHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCHHHHH
MADYLSEVEKLRLTYVSLISDIASRYYEWLDIEQRYSVSIDTVGLRQKELDIAKLRHANG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
VISGLDVRQAEVELQSTKVTLPTLDYERKYKINQLHILLGEYDYALPSPQGLPENMGLPY
CEECCCCHHHHEEEECCEEEECCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCE
ELNTGVPSELLTLRPDVKIAEQQMIAANAEVGIAKAAFFPKFTISGVYGRENDHLKDIFD
EECCCCCHHHHEECCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHC
SNGVTWSLLGGISAPIFNRGKIAAEYDIATEAAKQSMLSYRNVVLTAYFDVNDALNNLKR
CCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
AQEAIKAQKELVESSSAYARLARLRYQNGVATSLDLMDAQRQLFSAQLAYSEILRDKQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIALYRALGGGAVSE
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]