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The map label for this gene is emrD [H]

Identifier: 117920434

GI number: 117920434

Start: 2367658

End: 2368902

Strand: Reverse

Name: emrD [H]

Synonym: Shewana3_1989

Alternate gene names: 117920434

Gene position: 2368902-2367658 (Counterclockwise)

Preceding gene: 117920435

Following gene: 117920431

Centisome position: 47.64

GC content: 52.05

Gene sequence:

>1245_bases
ATGATGTCAGACGATGCACCGTCTTTAATCTCACAAAAAGCGCCGACACCCGCCGCAGGCAGCGCCACTCAGTTACTCTT
TATGCTGGTATTTATGGTGGCCTGTGGGCAGATGGCACAAACTATTTTTGTCCCCGCACTCCCCCTTATCGCCCAAGGCT
TAGCTGTGGATGCCAGTAAATTGCAGGCGGTCATGGCTTGCTATCTGCTCGCCTACGGCCTATGTCAGTTTATCTATGGC
CCATTATCCGACCGAGTTGGGCGTAAAATGCCGCTGCTCATTGGCATTGGGATTTTTATCCTTGGCGCCTTGATGGCGGC
AGAGGCTACAAGCTTTAATCAATTGATTTTCGCAAGCCTGCTACAGGGGTTAGGAACCGCGAGTGCGGGCGCCTTGTGCC
GCAGTATTCCAAGGGACCATTACTTCGGCGATAACTTAGTTAGATTTAACAGTTATGTGTCCATGGCCGTGGTGTTCTTG
CCCTTGGTCGCGCCCTTCTTAGGCAGTTTTGCCTCAGCTTACTGGGGGGTGCAGGCTGTGTATTGGGTGCTGTTTGGATT
TGGCAGTTTGGTTTGGCTACTGCTCTGCTTTGGTTTTAAGGAATCTTTACCCAAGCATAAGCGAGAGCAACAACCCATAT
TGGCATCCTATCGACATGTACTGCGCCACCGCAGTTTTCGAGCCTATTTGCTTTCGCTTATCGCGACCTTTGCAGGCATT
GCCGCCTTCGAAGCCATTGCGGGCGTACTCTATGGCCAATATTTGCAGCTCTCGCCGTTTATGGTGAGCTGTTATTTTGT
CGCGCCCATCCCTGGATATTTACTCGGCGCCCTGATTGCTGGTAGGCAAAAACAGGCGCAGCGTACGGTTTTACAAGGCG
TTGCACTGCTAGGCGCCGGCGCACTGTTTATGTTGATCCCCGGTATTTTTCAGCTGGTCGTGGGTTGGAGTTTATTGATA
GGCTCAATTTTGTTTTTCAGTGGCGCTGGCATGTTATTTCCGACATTAACCTCGGCCGCGCTAGAGCCGTTTCCACGCCA
AGCAGGCATTGCGGGCGCCCTACTCGGTGGCCTACAAAACTTCGGCGCGGGACTCGCTGCCTTAACCATGTCGCTCGTAC
CAATGGGCGGCCAGTTAAGCATTGGTTTACTGAGCGCGCTGATGGTGCTGTTAGTGATTGTCGCCTTGCATTATGCAAGG
GATCAAAACGATACCCACCACACCCTGTTACCGCAAAATCCATAA

Upstream 100 bases:

>100_bases
TTAAAAAATATTCGCGTAAATCAATATGATTGCCACTTGAACTGAAATGTTTTGGGGGTAAGATGCGCGCCATCGTTCAA
CTCAGCTTTTTAATGGAATT

Downstream 100 bases:

>100_bases
TTGGCAAGACACCCAATAAAAAGCCCACATTCGTGGGCTTTTTATTGGGGCGATTAGAGAATGCGTCTGAGCAGGAAATC
TGCTTTTAGCCTTCTAATAC

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MMSDDAPSLISQKAPTPAAGSATQLLFMLVFMVACGQMAQTIFVPALPLIAQGLAVDASKLQAVMACYLLAYGLCQFIYG
PLSDRVGRKMPLLIGIGIFILGALMAAEATSFNQLIFASLLQGLGTASAGALCRSIPRDHYFGDNLVRFNSYVSMAVVFL
PLVAPFLGSFASAYWGVQAVYWVLFGFGSLVWLLLCFGFKESLPKHKREQQPILASYRHVLRHRSFRAYLLSLIATFAGI
AAFEAIAGVLYGQYLQLSPFMVSCYFVAPIPGYLLGALIAGRQKQAQRTVLQGVALLGAGALFMLIPGIFQLVVGWSLLI
GSILFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSALMVLLVIVALHYAR
DQNDTHHTLLPQNP

Sequences:

>Translated_414_residues
MMSDDAPSLISQKAPTPAAGSATQLLFMLVFMVACGQMAQTIFVPALPLIAQGLAVDASKLQAVMACYLLAYGLCQFIYG
PLSDRVGRKMPLLIGIGIFILGALMAAEATSFNQLIFASLLQGLGTASAGALCRSIPRDHYFGDNLVRFNSYVSMAVVFL
PLVAPFLGSFASAYWGVQAVYWVLFGFGSLVWLLLCFGFKESLPKHKREQQPILASYRHVLRHRSFRAYLLSLIATFAGI
AAFEAIAGVLYGQYLQLSPFMVSCYFVAPIPGYLLGALIAGRQKQAQRTVLQGVALLGAGALFMLIPGIFQLVVGWSLLI
GSILFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSALMVLLVIVALHYAR
DQNDTHHTLLPQNP
>Mature_414_residues
MMSDDAPSLISQKAPTPAAGSATQLLFMLVFMVACGQMAQTIFVPALPLIAQGLAVDASKLQAVMACYLLAYGLCQFIYG
PLSDRVGRKMPLLIGIGIFILGALMAAEATSFNQLIFASLLQGLGTASAGALCRSIPRDHYFGDNLVRFNSYVSMAVVFL
PLVAPFLGSFASAYWGVQAVYWVLFGFGSLVWLLLCFGFKESLPKHKREQQPILASYRHVLRHRSFRAYLLSLIATFAGI
AAFEAIAGVLYGQYLQLSPFMVSCYFVAPIPGYLLGALIAGRQKQAQRTVLQGVALLGAGALFMLIPGIFQLVVGWSLLI
GSILFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSALMVLLVIVALHYAR
DQNDTHHTLLPQNP

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=351, Percent_Identity=43.3048433048433, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788509, Length=355, Percent_Identity=26.4788732394366, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI48994889, Length=372, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790146, Length=385, Percent_Identity=24.1558441558442, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44178; Mature: 44178

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]