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The map label for this gene is pflA [H]

Identifier: 117920002

GI number: 117920002

Start: 1810497

End: 1811237

Strand: Reverse

Name: pflA [H]

Synonym: Shewana3_1554

Alternate gene names: 117920002

Gene position: 1811237-1810497 (Counterclockwise)

Preceding gene: 117920003

Following gene: 117920000

Centisome position: 36.43

GC content: 47.1

Gene sequence:

>741_bases
ATGGCAGTTACCGGTCGGATCCACTCAGTGGAATCCTTTGGCACAGTGGATGGTCCAGGCATACGGTTTATCACCTTTAT
GCAGGGCTGTTTAATGCGCTGTCAGTATTGTCATAACCGTGATACTTGGGATCTTGATGGTGGCAAGGAAGTACAGGTCG
ACGAGTTAATGAGCCAAATCATTAGCTACCGTCCCTTCCTAGATGCCAGCAACGGCGGTGTGACCGCCAGCGGTGGCGAA
GCCATATTACAGGCGGAGTTTGTCGCCGAACTCTTTAAAGCCTGTAAAAAAGAAGGCATTCATACCTGTTTAGATACCAA
CGGCTTTGTGCGTAAGTACACGCCAGTGATTGATGAGCTACTCGATAATACCGATCTGGTGTTGCTCGATATCAAACAAA
TGAATGACGATAAACACATTGAACTGACTAAGGTCAGTAACCACAGAACGCTGCAATTTGCCGAGTATCTCGCCAAACGC
AATCAGCCTACCTGGATACGTTATGTGGTCGTGGGTGGATTTACTGACGATGAAGCTTCGGCATTACAGCTGGCCGAATT
TATCAAGCCCATGAAGAATATTGAAAAAGTTGAACTCCTGCCCTACCACGAACTGGGTAAACATAAGTGGGAAGCGATGG
GAGAAACCTATCAACTCGATGGCGTTGCACCACCGAGTCGCGACACAATGGAAAAAATTAAAGCCGTGTTTAGCTCACAG
GGGATTAATGCGGTGTATTAA

Upstream 100 bases:

>100_bases
AGACGTGATCACACGTACTTTCACAAAAGGTCTGTAAGCTAAATTAGGTAAGGCTGTAGTACAATGTGCTACAGCCTCAC
TTATTTCAACAGGAGTCACA

Downstream 100 bases:

>100_bases
GACGCCAAGGGAGGCTTAAAGCTCCTCCCAATAACTGGCATCTAAGCGCTCAAAGGCTATTTTTAAGATGAGCGCCATAT
CAAGGTAACACGCCTTAGCG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVTGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVQVDELMSQIISYRPFLDASNGGVTASGGE
AILQAEFVAELFKACKKEGIHTCLDTNGFVRKYTPVIDELLDNTDLVLLDIKQMNDDKHIELTKVSNHRTLQFAEYLAKR
NQPTWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGETYQLDGVAPPSRDTMEKIKAVFSSQ
GINAVY

Sequences:

>Translated_246_residues
MAVTGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVQVDELMSQIISYRPFLDASNGGVTASGGE
AILQAEFVAELFKACKKEGIHTCLDTNGFVRKYTPVIDELLDNTDLVLLDIKQMNDDKHIELTKVSNHRTLQFAEYLAKR
NQPTWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGETYQLDGVAPPSRDTMEKIKAVFSSQ
GINAVY
>Mature_245_residues
AVTGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVQVDELMSQIISYRPFLDASNGGVTASGGEA
ILQAEFVAELFKACKKEGIHTCLDTNGFVRKYTPVIDELLDNTDLVLLDIKQMNDDKHIELTKVSNHRTLQFAEYLAKRN
QPTWIRYVVVGGFTDDEASALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGETYQLDGVAPPSRDTMEKIKAVFSSQG
INAVY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=68.6991869918699, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1790389, Length=272, Percent_Identity=25, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI226510931, Length=270, Percent_Identity=27.037037037037, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27687; Mature: 27556

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00018 EF_HAND_1 ; PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVQVDELMSQI
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHH
ISYRPFLDASNGGVTASGGEAILQAEFVAELFKACKKEGIHTCLDTNGFVRKYTPVIDEL
HHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
LDNTDLVLLDIKQMNDDKHIELTKVSNHRTLQFAEYLAKRNQPTWIRYVVVGGFTDDEAS
HCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHH
ALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGETYQLDGVAPPSRDTMEKIKAVFSSQ
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCC
GINAVY
CCCCCC
>Mature Secondary Structure 
AVTGRIHSVESFGTVDGPGIRFITFMQGCLMRCQYCHNRDTWDLDGGKEVQVDELMSQI
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHH
ISYRPFLDASNGGVTASGGEAILQAEFVAELFKACKKEGIHTCLDTNGFVRKYTPVIDEL
HHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
LDNTDLVLLDIKQMNDDKHIELTKVSNHRTLQFAEYLAKRNQPTWIRYVVVGGFTDDEAS
HCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHH
ALQLAEFIKPMKNIEKVELLPYHELGKHKWEAMGETYQLDGVAPPSRDTMEKIKAVFSSQ
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCC
GINAVY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]