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The map label for this gene is ackA [H]

Identifier: 117920000

GI number: 117920000

Start: 1808697

End: 1809896

Strand: Reverse

Name: ackA [H]

Synonym: Shewana3_1552

Alternate gene names: 117920000

Gene position: 1809896-1808697 (Counterclockwise)

Preceding gene: 117920002

Following gene: 117919999

Centisome position: 36.4

GC content: 49.25

Gene sequence:

>1200_bases
ATGTCTAATAAACTGGTTTTAGTACTCAATTGCGGTAGCTCTTCACTCAAATTTGCCGTCATTGACGCGCAAACAGGTGA
CGATCAGATCTCTGGCCTAGCCGAGTGTTTTGGCTTAGAAGACTCGCGCATTAAATGGAAAATCAACGGTGAAAAGCATG
AATCCTCTTTAGGTGCATTTACCGCTCACCGCGAAGCCGTTGAATTTATTGTCAATAAAATCCTTGCTGGTCAACCTGAA
TTAGCCGCGCAGATCCAAGCCGTGGGTCACCGTATCGTACACGGCGGTGAGAAGTTCACTCGCTCTGTGATCATCGACGA
GCATGTGATCAAAGGTATTGAAGAATGTTCTTCACTGGCGCCACTGCATAACCCAGCACACCTTATCGGTATTCGCGCGG
CCATTGCATCTTTCCCTAAACTGCCACAAGTGGCGGTATTCGATACTGCATTCCACCAAAGCATGCCTGAGCGCGCGTAC
ATCTACGCTCTACCATACAAGCTGTACCGTGAGCATGGTATTCGCCGTTATGGTATGCACGGCACTAGCCACCTGTTTGT
GAGTCGTGAAGCCGCTAAAGTGTTGAACAAACCTTTAGAAGAAACCAATGTGATCTGCGCGCATTTAGGTAACGGCGCCT
CGGTGACTGCGGTTAAAGGCGGTAAGAGCGTTGATACCTCAATGGGACTGACGCCGCTTGAAGGTTTAGTCATGGGTACT
CGCTGTGGCGATCTCGACCCCTCTATCATCTACCACTTAGTGCACCAACTGGGTTACACACTGGAAGAAGTCAACAACCT
GATGAACAAGCAAAGTGGTCTGCTAGGGATTTCTGAGCTGACCAACGATTGCCGTGGCATCGAAGAAGGTTATGCCGATG
GTCACAAAGGCGCGACCTTAGCACTGGAAATCTTCTGCTACCGTCTGGCGAAATATATCGCGTCTTACACTGTGCCGCTC
GGTCGTTTAGATGCCGTAGTCTTTACCGGTGGTATCGGCGAAAACTCCGACATCATCCGCGAGAAAGTGCTCAACATGCT
GCAAATCTTCAATTTCCATGTGGATAGCGAGCGTAACAAAGCGGCCCGCTTCGGCAAGAAAGGCATCATCACCACTGACA
ACAGTACCGTTGCCATGGTCATCCCGACCAACGAAGAGTGGGTTATCGCCGAAGATTCGATTAAACTCATTACAAAATAA

Upstream 100 bases:

>100_bases
CACAATCTGGCACTGCGCCCCAAGACTGTGATGGCAAATTACGCAAGCGTTCCCAAGATTTTGAATCGTTAACTTAAACA
CAATGGCAAAAGGTTTACAC

Downstream 100 bases:

>100_bases
TAAGATGATTCAAGCCGTTTGCTCAGCCAAACGGCTTTATTATTAGCGACCTACAGCTAAATAAACACAAGCAAAGCAGA
GACGACTCACTCTGCCAAAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSNKLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHESSLGAFTAHREAVEFIVNKILAGQPE
LAAQIQAVGHRIVHGGEKFTRSVIIDEHVIKGIEECSSLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPERAY
IYALPYKLYREHGIRRYGMHGTSHLFVSREAAKVLNKPLEETNVICAHLGNGASVTAVKGGKSVDTSMGLTPLEGLVMGT
RCGDLDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDIIREKVLNMLQIFNFHVDSERNKAARFGKKGIITTDNSTVAMVIPTNEEWVIAEDSIKLITK

Sequences:

>Translated_399_residues
MSNKLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHESSLGAFTAHREAVEFIVNKILAGQPE
LAAQIQAVGHRIVHGGEKFTRSVIIDEHVIKGIEECSSLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPERAY
IYALPYKLYREHGIRRYGMHGTSHLFVSREAAKVLNKPLEETNVICAHLGNGASVTAVKGGKSVDTSMGLTPLEGLVMGT
RCGDLDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDIIREKVLNMLQIFNFHVDSERNKAARFGKKGIITTDNSTVAMVIPTNEEWVIAEDSIKLITK
>Mature_398_residues
SNKLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHESSLGAFTAHREAVEFIVNKILAGQPEL
AAQIQAVGHRIVHGGEKFTRSVIIDEHVIKGIEECSSLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPERAYI
YALPYKLYREHGIRRYGMHGTSHLFVSREAAKVLNKPLEETNVICAHLGNGASVTAVKGGKSVDTSMGLTPLEGLVMGTR
CGDLDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPLG
RLDAVVFTGGIGENSDIIREKVLNMLQIFNFHVDSERNKAARFGKKGIITTDNSTVAMVIPTNEEWVIAEDSIKLITK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=63.6591478696742, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=45.7286432160804, Blast_Score=342, Evalue=3e-95,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43582; Mature: 43450

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHESSLGAF
CCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
TAHREAVEFIVNKILAGQPELAAQIQAVGHRIVHGGEKFTRSVIIDEHVIKGIEECSSLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPERAYIYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKVLNKPLEETNVICAHLGNGASVTAVKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHHCCHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDLDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDIIREKVLNMLQIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITTDNSTVAMVIPTNEEWVIAEDSIKLITK
HHHCCCCCEEEECCCEEEEEEECCCCEEEECCCEEEECC
>Mature Secondary Structure 
SNKLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHESSLGAF
CCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
TAHREAVEFIVNKILAGQPELAAQIQAVGHRIVHGGEKFTRSVIIDEHVIKGIEECSSLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPERAYIYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKVLNKPLEETNVICAHLGNGASVTAVKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHHCCHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDLDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDIIREKVLNMLQIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITTDNSTVAMVIPTNEEWVIAEDSIKLITK
HHHCCCCCEEEECCCEEEEEEECCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA