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The map label for this gene is mnmC [H]

Identifier: 117919922

GI number: 117919922

Start: 1712516

End: 1714438

Strand: Reverse

Name: mnmC [H]

Synonym: Shewana3_1474

Alternate gene names: 117919922

Gene position: 1714438-1712516 (Counterclockwise)

Preceding gene: 117919932

Following gene: 117919915

Centisome position: 34.48

GC content: 51.85

Gene sequence:

>1923_bases
ATGGGGCAATGGGGATTGGGCGATGGACTCGAGCTGCTCTCTTTACTGCATCACTGGCAAACGCAGACTCAAAGCAACAC
ACGGCTGTTAGTGAAAGTGTTTGAGCCCAATCCCATTAACGATTACGAACTTAAATTACTTTGGGATCAATCGCAAAGCT
TAATTTCCACGCCTCATTTACAACCGATTGCCAATGCCATCCTTAAGGCAAAACCTGCGCGCATTATAGGGTGCCAGAGA
TTAATCTTTGATGACGGCCGCATCACGGTCGATTTACATTTTGGCGACCTGCACACCTCACTCACCAATTTGCCCCACAG
CCCTGCTCATCCTATTCAGCAATGGCTGGTTTTACCGCATTTGGCATCGCAGTTAAGCGGCAAACTAGCGTGGCAAATGG
CGCGTTTAAGTGCCGATGATGCCCAGCTGATTGGGGTCAATTTGGCAGAAACCGTGCAGCAGCTTGCCCACAGCAGTGGC
TTTAGCACCCTTAATGTCAGCCAAGATGCCTTGAATGGTGATGCAAGTGACGCACTGCCAAGCCAGATTATCACCGATGA
GATTTTGCTCCATGAGCGCAAACTGCTGCGCCAACAAGCCGATACCGCTCAGGCATTTACCCCAAAACCTGCCACGCTCG
CGGCTATAGATCACCCTGTTGCCATCGTCGGTGGCGGACTGGCCTCAGCGAACCTAATGCTATCCCTCGCCGAACGAGGC
CAGAGTAGCACATTGTTTTGTAAGGATAATGAGTTAGGTCAAGGCGCATCAGGCAATCGTCAAGGAGCAATTTACCCCCT
ACTCACGCCTGAAAATGATGAGCTTAGCCGTTTTTTCCAACAGGCCTTTTTGTTCAGCCGCCGCCGTATTGAGGCGCTGA
GCCAAGCGTCGATGATGGATACAAACGCCGCCCCTCATGTTACGGTGATTTCCCATGATTTTTGTGGGGTACTGCAAACG
GGTCATGATGAGCGCAGCCAACAACGGCTGGATAAAATCATCCAAAGTCAGGACTGGCCGGCGGAAATCGCCTACGCCGT
TGATGCGAATGAAGCCAACGAAATAGCCCAAATAGGTATTGATAAGGCTGGATTTTTTTATCCACTCGGAGGCTGGGTAT
GCCCATTTGAATACGCCAAGGCGGCGGTGGACAAGGCCTCGCAACTTGCCAATGTGCAGTGCCATTTCAATACCGAGATC
ACCGAAATTGAATGTGATGCACAGGCTTGGTATCTTCACAGTCAAGGCCAGCGTTTTGGACCATTCCGCCAACTCGTGCT
CGCCAATGGCGCGCAGCTCACCCAATTTTCGGCCAGTGAGCGATTACAGATAAGTCCGTTTCGCGGCCAAGTGAGCCACG
TCCCCGCCCAATTTAAATTGAGCCAATTGGCGACAGTGTTATGTGCGAACGGTTATCTCACGCCGAGTCATCAAGGCTTG
CATTGCCTCGGCGCCAGTTATGTCAAAGCGGCAGAGCACTTCGATTTTTGCCCGCAGGAGCAACGGGAAAACCTCGGGAA
AATGCAAGAAAGCTACCCCAACCAAGCCTGGGTTGACGATATCGATATCAGTGGCAATAGCGCTCGTGTCGGCGTGCGGA
TGGTGACGCGGGATCATTTCCCCATGATGGGCTGTGCGCCCGATGTGGCTGAAATATTGGCGCGCTATGAACTGCATCAA
CTCAATCAACAGCAGGCCGAACAAAGCAAGCATTATTGGCAAACAACCCCCGCGCCTATCCTCGATGGCCTTTATATTTT
GGGTGGTTTAGGGTCGCGGGGATTAAGTTCAGGCCCCTTGGCGGCCGAATGCTTGGCGGCACAGTTAACCGGAGAGCCGC
TGCCACTGGATTGGTCAACCTTAAATAAACTCAACCCCAATCGGATGTGGCTACGCAAATTACTAAAGGGGAAAGCGCTT
TAA

Upstream 100 bases:

>100_bases
ATGCGCGTTGACTACTGCACAGTCTCTGGACAATTTTACTCGCCTTCGACGCTCTCGCTTAACGACGCCATCAGCCCAAC
TCGGTCAGGAACTGTACGTC

Downstream 100 bases:

>100_bases
TAAAAAGCGGTTTGGTAAAAAGTGCTTTAGTCAAGATAAGCATCGTGGTTTGAGTAAAGCCTGTGTAATTAAAACAAGGT
TTATCATTAAGCGCTAATCA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MGQWGLGDGLELLSLLHHWQTQTQSNTRLLVKVFEPNPINDYELKLLWDQSQSLISTPHLQPIANAILKAKPARIIGCQR
LIFDDGRITVDLHFGDLHTSLTNLPHSPAHPIQQWLVLPHLASQLSGKLAWQMARLSADDAQLIGVNLAETVQQLAHSSG
FSTLNVSQDALNGDASDALPSQIITDEILLHERKLLRQQADTAQAFTPKPATLAAIDHPVAIVGGGLASANLMLSLAERG
QSSTLFCKDNELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRIEALSQASMMDTNAAPHVTVISHDFCGVLQT
GHDERSQQRLDKIIQSQDWPAEIAYAVDANEANEIAQIGIDKAGFFYPLGGWVCPFEYAKAAVDKASQLANVQCHFNTEI
TEIECDAQAWYLHSQGQRFGPFRQLVLANGAQLTQFSASERLQISPFRGQVSHVPAQFKLSQLATVLCANGYLTPSHQGL
HCLGASYVKAAEHFDFCPQEQRENLGKMQESYPNQAWVDDIDISGNSARVGVRMVTRDHFPMMGCAPDVAEILARYELHQ
LNQQQAEQSKHYWQTTPAPILDGLYILGGLGSRGLSSGPLAAECLAAQLTGEPLPLDWSTLNKLNPNRMWLRKLLKGKAL

Sequences:

>Translated_640_residues
MGQWGLGDGLELLSLLHHWQTQTQSNTRLLVKVFEPNPINDYELKLLWDQSQSLISTPHLQPIANAILKAKPARIIGCQR
LIFDDGRITVDLHFGDLHTSLTNLPHSPAHPIQQWLVLPHLASQLSGKLAWQMARLSADDAQLIGVNLAETVQQLAHSSG
FSTLNVSQDALNGDASDALPSQIITDEILLHERKLLRQQADTAQAFTPKPATLAAIDHPVAIVGGGLASANLMLSLAERG
QSSTLFCKDNELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRIEALSQASMMDTNAAPHVTVISHDFCGVLQT
GHDERSQQRLDKIIQSQDWPAEIAYAVDANEANEIAQIGIDKAGFFYPLGGWVCPFEYAKAAVDKASQLANVQCHFNTEI
TEIECDAQAWYLHSQGQRFGPFRQLVLANGAQLTQFSASERLQISPFRGQVSHVPAQFKLSQLATVLCANGYLTPSHQGL
HCLGASYVKAAEHFDFCPQEQRENLGKMQESYPNQAWVDDIDISGNSARVGVRMVTRDHFPMMGCAPDVAEILARYELHQ
LNQQQAEQSKHYWQTTPAPILDGLYILGGLGSRGLSSGPLAAECLAAQLTGEPLPLDWSTLNKLNPNRMWLRKLLKGKAL
>Mature_639_residues
GQWGLGDGLELLSLLHHWQTQTQSNTRLLVKVFEPNPINDYELKLLWDQSQSLISTPHLQPIANAILKAKPARIIGCQRL
IFDDGRITVDLHFGDLHTSLTNLPHSPAHPIQQWLVLPHLASQLSGKLAWQMARLSADDAQLIGVNLAETVQQLAHSSGF
STLNVSQDALNGDASDALPSQIITDEILLHERKLLRQQADTAQAFTPKPATLAAIDHPVAIVGGGLASANLMLSLAERGQ
SSTLFCKDNELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRIEALSQASMMDTNAAPHVTVISHDFCGVLQTG
HDERSQQRLDKIIQSQDWPAEIAYAVDANEANEIAQIGIDKAGFFYPLGGWVCPFEYAKAAVDKASQLANVQCHFNTEIT
EIECDAQAWYLHSQGQRFGPFRQLVLANGAQLTQFSASERLQISPFRGQVSHVPAQFKLSQLATVLCANGYLTPSHQGLH
CLGASYVKAAEHFDFCPQEQRENLGKMQESYPNQAWVDDIDISGNSARVGVRMVTRDHFPMMGCAPDVAEILARYELHQL
NQQQAEQSKHYWQTTPAPILDGLYILGGLGSRGLSSGPLAAECLAAQLTGEPLPLDWSTLNKLNPNRMWLRKLLKGKAL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=661, Percent_Identity=34.7957639939486, Blast_Score=348, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 70482; Mature: 70350

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQWGLGDGLELLSLLHHWQTQTQSNTRLLVKVFEPNPINDYELKLLWDQSQSLISTPHL
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHCCCCH
QPIANAILKAKPARIIGCQRLIFDDGRITVDLHFGDLHTSLTNLPHSPAHPIQQWLVLPH
HHHHHHHHHCCCCHHEEEEEEEEECCEEEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHH
LASQLSGKLAWQMARLSADDAQLIGVNLAETVQQLAHSSGFSTLNVSQDALNGDASDALP
HHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCH
SQIITDEILLHERKLLRQQADTAQAFTPKPATLAAIDHPVAIVGGGLASANLMLSLAERG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCC
QSSTLFCKDNELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRIEALSQASMMD
CCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TNAAPHVTVISHDFCGVLQTGHDERSQQRLDKIIQSQDWPAEIAYAVDANEANEIAQIGI
CCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCC
DKAGFFYPLGGWVCPFEYAKAAVDKASQLANVQCHFNTEITEIECDAQAWYLHSQGQRFG
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCEEEEEECCCCCCC
PFRQLVLANGAQLTQFSASERLQISPFRGQVSHVPAQFKLSQLATVLCANGYLTPSHQGL
HHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
HCLGASYVKAAEHFDFCPQEQRENLGKMQESYPNQAWVDDIDISGNSARVGVRMVTRDHF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEEEEEECCCC
PMMGCAPDVAEILARYELHQLNQQQAEQSKHYWQTTPAPILDGLYILGGLGSRGLSSGPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
AAECLAAQLTGEPLPLDWSTLNKLNPNRMWLRKLLKGKAL
HHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
GQWGLGDGLELLSLLHHWQTQTQSNTRLLVKVFEPNPINDYELKLLWDQSQSLISTPHL
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHCCCCH
QPIANAILKAKPARIIGCQRLIFDDGRITVDLHFGDLHTSLTNLPHSPAHPIQQWLVLPH
HHHHHHHHHCCCCHHEEEEEEEEECCEEEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHH
LASQLSGKLAWQMARLSADDAQLIGVNLAETVQQLAHSSGFSTLNVSQDALNGDASDALP
HHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCH
SQIITDEILLHERKLLRQQADTAQAFTPKPATLAAIDHPVAIVGGGLASANLMLSLAERG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCC
QSSTLFCKDNELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRIEALSQASMMD
CCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TNAAPHVTVISHDFCGVLQTGHDERSQQRLDKIIQSQDWPAEIAYAVDANEANEIAQIGI
CCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCC
DKAGFFYPLGGWVCPFEYAKAAVDKASQLANVQCHFNTEITEIECDAQAWYLHSQGQRFG
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCEEEEEECCCCCCC
PFRQLVLANGAQLTQFSASERLQISPFRGQVSHVPAQFKLSQLATVLCANGYLTPSHQGL
HHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
HCLGASYVKAAEHFDFCPQEQRENLGKMQESYPNQAWVDDIDISGNSARVGVRMVTRDHF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEEEEEECCCC
PMMGCAPDVAEILARYELHQLNQQQAEQSKHYWQTTPAPILDGLYILGGLGSRGLSSGPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
AAECLAAQLTGEPLPLDWSTLNKLNPNRMWLRKLLKGKAL
HHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA