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The map label for this gene is putP [H]

Identifier: 117919899

GI number: 117919899

Start: 1682588

End: 1684039

Strand: Reverse

Name: putP [H]

Synonym: Shewana3_1451

Alternate gene names: 117919899

Gene position: 1684039-1682588 (Counterclockwise)

Preceding gene: 117919900

Following gene: 117919898

Centisome position: 33.87

GC content: 50.9

Gene sequence:

>1452_bases
ATGACGATTGAAACCCCGATTTTAATCACATTTGTTGGCTATTTAGTATTGATGATGGGCATAGGATTTTGGGCTTACCG
TGCCACCGATACTGTTGATGATTATATTTTAGGTGGCCGTAAAATGGGCCCCGCCGTAACCGCGCTCAGCGTGGGCGCCT
CCGATATGTCAGGCTGGTTACTCTTAGGTTTACCCGGCGCCGTTTACTTAGGCGGCTTAGGCGAAGCTTGGATTGGTATC
GGCTTAGTCGTCGGTGCTTGGCTTAACTGGTTGTTTGTGGCCAAACGTTTGCGCATTTACACTCAATTAGCGGACAACGC
CCTCACCCTGCCCGACTTCTTCGAGAAACGTTTTCACGATAAACAGGGCTACCTCAAACTCGTCTCTGCGGTCACGATTC
TGGTGTTTTTCACCTTCTATGCATCCTCTGGCATGGTGGGCGGCGCCATTCTGTTCGAAAAAGTCTTCGGACTCGATTAC
ACCGTTGCCTTAGTCATTGGTTCGGCCATCATCGTGGGTTACACCTTTATTGGCGGCTTTTTTGCCGTGAGTTGGACCGA
CTTCTTCCAAGGCTGCTTGATGCTGATAGCCCTGCTGATCATCCCCTTTGCGGTGTTCTCGCACCCAGAGAGCCATGCGG
GAATTGAGTCTATCGACCCGGCTATGCTGGCACTTATCAGTGACAAAACCACGGTAATTGGCTTGTTATCACTGCTGGCG
TGGGGACTAGGTTATTTCGGCCAGCCACATATTTTGTCGCGCTTTATGGCCATTGGCACTGCCGATGCACTACCGCTGTC
TCGCCGTATCGCCATGAGCTGGATGATGTTATCCCTCATCGGCGCTTTAGCCACAGGGTTAGCGGGTTCACTGTATTTTG
CCAATCAACCACTCGCCAATGCCGAAACCGTGTTTATTCATTTAGCCCAAGCCGCCTTTAACCCTTGGATTGGGGGCTTG
CTGATCGCCGCAATTCTATCGGCCATTATGAGTACCATTGATTCGCAGTTATTGGTGTGCTCGAGCGTCATCACCGAAGA
CTTTTACCGCAAGTGGTTGCGCCCTCAAGCCGACGATCGCGAGTTGATGATGGTAGGTCGTATGGGCGTGCTGGCCATTG
CCGTGATCGCAGGCATCATTGCATTGAATCCTGAAAGCAGTGTATTAAGCCTTGTGAGCTATGCGTGGGCAGGTTTTGGC
GCTGCCTTTGGCCCTGTGGTGCTCCTGTCACTCTTCTGGAAGCAATACAGCCGTAACGGTGCAATTGCTACCATTATTGT
CGGCGCATTAACCGTGGTGATATGGAAGCAGCTCACAGGTGGCATTTTCGACCTATACGAAATCTTACCTGGATTTGTGT
TTGCAATTATTGCAGGGGTTGTTGTGAGCAAGATGTCTCGCCCAGCAGAAACCATCACCGCAGAGTTTGAGCAATTTAAG
TCCGCGTTGTAA

Upstream 100 bases:

>100_bases
TCGGATTTCGGTCACATCTTTATCTACTAATGGGTTCTTGCTCAAACATCTGGTAGCATGCTTGCCACTTTTAATAATAA
CAAGATGGGATAAAGACAAT

Downstream 100 bases:

>100_bases
CAGCTGAAATAATTTCTGAGAACGTAAAAAGCGTACAAGTGTACGCTTTTTACAATTTGTATAAATTTATTAACCTTTCA
TCAGTTACAAAACGTTAACC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVVGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKQGYLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDY
TVALVIGSAIIVGYTFIGGFFAVSWTDFFQGCLMLIALLIIPFAVFSHPESHAGIESIDPAMLALISDKTTVIGLLSLLA
WGLGYFGQPHILSRFMAIGTADALPLSRRIAMSWMMLSLIGALATGLAGSLYFANQPLANAETVFIHLAQAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFG
AAFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFDLYEILPGFVFAIIAGVVVSKMSRPAETITAEFEQFK
SAL

Sequences:

>Translated_483_residues
MTIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVVGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKQGYLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDY
TVALVIGSAIIVGYTFIGGFFAVSWTDFFQGCLMLIALLIIPFAVFSHPESHAGIESIDPAMLALISDKTTVIGLLSLLA
WGLGYFGQPHILSRFMAIGTADALPLSRRIAMSWMMLSLIGALATGLAGSLYFANQPLANAETVFIHLAQAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFG
AAFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFDLYEILPGFVFAIIAGVVVSKMSRPAETITAEFEQFK
SAL
>Mature_482_residues
TIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGIG
LVVGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKQGYLKLVSAVTILVFFTFYASSGMVGGAILFEKVFGLDYT
VALVIGSAIIVGYTFIGGFFAVSWTDFFQGCLMLIALLIIPFAVFSHPESHAGIESIDPAMLALISDKTTVIGLLSLLAW
GLGYFGQPHILSRFMAIGTADALPLSRRIAMSWMMLSLIGALATGLAGSLYFANQPLANAETVFIHLAQAAFNPWIGGLL
IAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFGA
AFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFDLYEILPGFVFAIIAGVVVSKMSRPAETITAEFEQFKS
AL

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=484, Percent_Identity=46.900826446281, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4507035, Length=376, Percent_Identity=24.468085106383, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI11141885, Length=399, Percent_Identity=25.8145363408521, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI17941285, Length=461, Percent_Identity=25.3796095444685, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI256985183, Length=442, Percent_Identity=23.7556561085973, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1787251, Length=494, Percent_Identity=53.0364372469636, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI87082237, Length=461, Percent_Identity=27.3318872017354, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790503, Length=460, Percent_Identity=25, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1790113, Length=406, Percent_Identity=23.1527093596059, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=412, Percent_Identity=23.7864077669903, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533094, Length=370, Percent_Identity=23.7837837837838, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24645928, Length=382, Percent_Identity=28.0104712041885, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI221459584, Length=367, Percent_Identity=23.1607629427793, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648033, Length=399, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356865, Length=399, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640370, Length=362, Percent_Identity=26.2430939226519, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52062; Mature: 51931

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCC
>Mature Secondary Structure 
TIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCEE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HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]