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The map label for this gene is flgJ [H]

Identifier: 117919777

GI number: 117919777

Start: 1541061

End: 1542230

Strand: Direct

Name: flgJ [H]

Synonym: Shewana3_1329

Alternate gene names: 117919777

Gene position: 1541061-1542230 (Clockwise)

Preceding gene: 117919776

Following gene: 117919778

Centisome position: 30.99

GC content: 46.5

Gene sequence:

>1170_bases
ATGGAAAAGCTTTCAAGTTCATCACATTTTCTTGACCTGGGCGGGCTAGATTCACTTCGCGCCCAGGCACAGAAGGATGA
GAAGGGCACTTTAAAACAAGTTGCCCAACAATTTGAAGGCATTTTCGTGCAAATGTTGATGAAAAGCATGCGCGATGCTA
ATGCGGTATTTGAATCGGATAGTCCCCTCAATAGCCAATACACTAAGTTTTATGAGCAAATGCGTGATCAGCAATTGTCG
GTCGATTTGTCGGATAAAGGTGTGTTAGGTCTTGCCGACATGATGGTGCAACAGTTATCACCTGAGTCTAGCCAGCATAT
TCCTGCCTCTGTATTACGTAGTGATGGCGGCGAAAAATTGCTGCAAGGCGATAAAGCATTGAGTGTTCCAGCGCAAAATA
CGGCAACCCAAGATGTGCTTAATGCTTCAGCAACGACTGCAAGCACTTCTGCAAGCACTTCTGCAGGCATCCCAGCCTAT
ATTGTTCGTCCAACCTTTGAGTCAGATAGGCCAGATGATGTGGCCTCGTCATTGGATATCGACACGCCAGCACCTTCATT
AGCGGTTAGTACACCCAAACCAACCTGGTCAGAGCAACCACTATCGCCCATTGAGCCTGTAATTAGCGGCCAGATTTTGC
CGACAGTGGCATTTAGAGAAACCCAAAAGACACTCAAATTTGGTAGTCGGGAAGAGTTTTTGGCGACCTTGTATCCCCAT
GCAGAAAAAGCGGCTAAGGCTTTGGGTACCCAACCTGAAGTGCTACTGGCGCAGTCGGCGCTCGAAACCGGTTGGGGACA
GAAAATTGTACGAGGTAGCAATGGTGCACCTAGTCACAACCTATTCAACATTAAGGCTGATCGCCGTTGGCTTGGGGATA
AGGCTAACGTGAGTACCTTGGAATTCGAGCAGGGTATTGCCGTTAGACAAAAAGCCGATTTTCGCGTTTACACTGATTTT
GAACATAGTTTTAACGATTTTGTCACCTTTATTGCTGAGGGTGAGCGTTATCAAGATGCTAAAAAAGTCGCCGCTTCACC
AACCCAATTTATTCGTGCATTGCAGGATGCGGGCTATGCCACCGATCCAAAATATGCTGAGAAAGTCATTAAAGTGATGC
AGACAATAAGCCAAGAGCTTAAGTCGATACTGCAAGGAGAAGACAAATGA

Upstream 100 bases:

>100_bases
GTGCAGCACCTTCAGATGTACTTGCTATCCTAGAAGCATTAAAAGTGGCAGGTGCTTTGCATGGTGAGCTTATCATCATC
TAATTTCTGTCGGGAAAATT

Downstream 100 bases:

>100_bases
TAAATCTTCATAGGTTATTAGTTCTGAGGAGTGTCGGCTAATGGCGATTGACCTATTAAATATTGCTCGTACTGGGGTGC
TAGCCTCGCAGTCGCAACTT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQHIPASVLRSDGGEKLLQGDKALSVPAQNTATQDVLNASATTASTSASTSAGIPAY
IVRPTFESDRPDDVASSLDIDTPAPSLAVSTPKPTWSEQPLSPIEPVISGQILPTVAFRETQKTLKFGSREEFLATLYPH
AEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDF
EHSFNDFVTFIAEGERYQDAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQTISQELKSILQGEDK

Sequences:

>Translated_389_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQHIPASVLRSDGGEKLLQGDKALSVPAQNTATQDVLNASATTASTSASTSAGIPAY
IVRPTFESDRPDDVASSLDIDTPAPSLAVSTPKPTWSEQPLSPIEPVISGQILPTVAFRETQKTLKFGSREEFLATLYPH
AEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDF
EHSFNDFVTFIAEGERYQDAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQTISQELKSILQGEDK
>Mature_389_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQHIPASVLRSDGGEKLLQGDKALSVPAQNTATQDVLNASATTASTSASTSAGIPAY
IVRPTFESDRPDDVASSLDIDTPAPSLAVSTPKPTWSEQPLSPIEPVISGQILPTVAFRETQKTLKFGSREEFLATLYPH
AEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDF
EHSFNDFVTFIAEGERYQDAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQTISQELKSILQGEDK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=156, Percent_Identity=43.5897435897436, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 42543; Mature: 42543

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQHIPASVLRSDGGEKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHH
LQGDKALSVPAQNTATQDVLNASATTASTSASTSAGIPAYIVRPTFESDRPDDVASSLDI
HCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCC
DTPAPSLAVSTPKPTWSEQPLSPIEPVISGQILPTVAFRETQKTLKFGSREEFLATLYPH
CCCCCCCEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCC
AEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTL
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCEE
EFEQGIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQDAKKVAASPTQFIRALQDAGYA
EHHCCCHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCC
TDPKYAEKVIKVMQTISQELKSILQGEDK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQHIPASVLRSDGGEKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHH
LQGDKALSVPAQNTATQDVLNASATTASTSASTSAGIPAYIVRPTFESDRPDDVASSLDI
HCCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCC
DTPAPSLAVSTPKPTWSEQPLSPIEPVISGQILPTVAFRETQKTLKFGSREEFLATLYPH
CCCCCCCEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCC
AEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTL
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCEE
EFEQGIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQDAKKVAASPTQFIRALQDAGYA
EHHCCCHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCC
TDPKYAEKVIKVMQTISQELKSILQGEDK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]