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The map label for this gene is ispD [H]

Identifier: 117919567

GI number: 117919567

Start: 1317457

End: 1318206

Strand: Direct

Name: ispD [H]

Synonym: Shewana3_1118

Alternate gene names: 117919567

Gene position: 1317457-1318206 (Clockwise)

Preceding gene: 117919566

Following gene: 117919568

Centisome position: 26.5

GC content: 54.53

Gene sequence:

>750_bases
ATGGATACCGTATTAGAGGCCGCGCCGCAGGTCGAAACCCAAGTTGCAGTTCAAATTGATCCTTTCTCGCACCATGTGGT
GGCGATTGTGCCCGCCGCGGGGATTGGCAGCCGCATGGGCGCGGGCAAACCCAAACAATATTTAACCTTGCTGGGACAAA
GCATTCTGGCTCATACCTTAGATAAGTTATTATCCCATCCGCAAATTAACCAAGTGATTGTGGCGCTGCATCCTGAGGAT
ACCAAGTTTGCCGCGTTGCCGCAGGCCAAGCATCCCAAGCTGGTAACTGTCATTGGCGGTTCTGAGCGTGCCGACTCGGT
GCTGGCGGCACTCGATAAGGCGCCTGATAATGGTTGGGCTTTAGTTCACGATGCGGCCAGACCCTGCCTGACGGCGCAAG
ACATTGATAAATTGTTGGCATCGCGGGTGCACTTTCCCCAAGGGGCGATTTTAGCCATGCCTGTGCGGGATACCATGAAG
CGCGCCAATAGCTTAGGTGAAATTAGCTCGACTGTTTGCCGCGATAATCTCTGGCATGCGTTGACACCGCAGTTATTCCC
AACCTCCTTGTTGCGATTACATTTAAAAGCCGCGCTTGCCGCGGGGGCTGTCGTGACCGATGAAGCCTCGGCGATGGAGT
GGGCGGGCATTTCCCCTGGTTTGGTCGCGGGGCGGGCGGATAACATTAAAGTCACCCATCCCGATGATTTGGAGTTGGCA
GAACTTTTTTTACTGCGCGCGAATGCTTGA

Upstream 100 bases:

>100_bases
GCGCGTAACGAGCTAGGCATGGTGAAAGAAGGCGAAACCTTCTATCGCGTGGTGGGCGGTGACCGTTCAGTATCGAGTCC
CTCGCAGTAACGGAGCGCCT

Downstream 100 bases:

>100_bases
AGCCAAGCAGAGTGAATTGCGCAATCTATTAAATACGTTAGGAAATAAGTAATGAAAATCCGAATCGGCCATGGTTTTGA
TGTCCATAAATTTGGTGAAG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MDTVLEAAPQVETQVAVQIDPFSHHVVAIVPAAGIGSRMGAGKPKQYLTLLGQSILAHTLDKLLSHPQINQVIVALHPED
TKFAALPQAKHPKLVTVIGGSERADSVLAALDKAPDNGWALVHDAARPCLTAQDIDKLLASRVHFPQGAILAMPVRDTMK
RANSLGEISSTVCRDNLWHALTPQLFPTSLLRLHLKAALAAGAVVTDEASAMEWAGISPGLVAGRADNIKVTHPDDLELA
ELFLLRANA

Sequences:

>Translated_249_residues
MDTVLEAAPQVETQVAVQIDPFSHHVVAIVPAAGIGSRMGAGKPKQYLTLLGQSILAHTLDKLLSHPQINQVIVALHPED
TKFAALPQAKHPKLVTVIGGSERADSVLAALDKAPDNGWALVHDAARPCLTAQDIDKLLASRVHFPQGAILAMPVRDTMK
RANSLGEISSTVCRDNLWHALTPQLFPTSLLRLHLKAALAAGAVVTDEASAMEWAGISPGLVAGRADNIKVTHPDDLELA
ELFLLRANA
>Mature_249_residues
MDTVLEAAPQVETQVAVQIDPFSHHVVAIVPAAGIGSRMGAGKPKQYLTLLGQSILAHTLDKLLSHPQINQVIVALHPED
TKFAALPQAKHPKLVTVIGGSERADSVLAALDKAPDNGWALVHDAARPCLTAQDIDKLLASRVHFPQGAILAMPVRDTMK
RANSLGEISSTVCRDNLWHALTPQLFPTSLLRLHLKAALAAGAVVTDEASAMEWAGISPGLVAGRADNIKVTHPDDLELA
ELFLLRANA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=56.3063063063063, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26491; Mature: 26491

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVLEAAPQVETQVAVQIDPFSHHVVAIVPAAGIGSRMGAGKPKQYLTLLGQSILAHTL
CCCHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DKLLSHPQINQVIVALHPEDTKFAALPQAKHPKLVTVIGGSERADSVLAALDKAPDNGWA
HHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEE
LVHDAARPCLTAQDIDKLLASRVHFPQGAILAMPVRDTMKRANSLGEISSTVCRDNLWHA
EEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHH
LTPQLFPTSLLRLHLKAALAAGAVVTDEASAMEWAGISPGLVAGRADNIKVTHPDDLELA
CCCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHCCCCCCEEECCCCCEEEECCCCHHHH
ELFLLRANA
HHHHHHCCC
>Mature Secondary Structure
MDTVLEAAPQVETQVAVQIDPFSHHVVAIVPAAGIGSRMGAGKPKQYLTLLGQSILAHTL
CCCHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DKLLSHPQINQVIVALHPEDTKFAALPQAKHPKLVTVIGGSERADSVLAALDKAPDNGWA
HHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEE
LVHDAARPCLTAQDIDKLLASRVHFPQGAILAMPVRDTMKRANSLGEISSTVCRDNLWHA
EEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHH
LTPQLFPTSLLRLHLKAALAAGAVVTDEASAMEWAGISPGLVAGRADNIKVTHPDDLELA
CCCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHCCCCCCEEECCCCCEEEECCCCHHHH
ELFLLRANA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]