Click here to switch to the map view.

The map label for this gene is pyrG [H]

Identifier: 117919564

GI number: 117919564

Start: 1313897

End: 1315537

Strand: Direct

Name: pyrG [H]

Synonym: Shewana3_1115

Alternate gene names: 117919564

Gene position: 1313897-1315537 (Clockwise)

Preceding gene: 117919563

Following gene: 117919565

Centisome position: 26.42

GC content: 47.96

Gene sequence:

>1641_bases
ATGACTACAAGGTATATCTTCGTTACTGGTGGTGTGGTTTCATCACTAGGTAAAGGCATTGCAGCCGCTTCATTAGCCGC
AATTTTAGAGGCCCGTGGTCTCAATGTAACCATCATGAAACTGGACCCATACATTAACGTTGATCCAGGTACCATGAGCC
CAACGCAACATGGTGAAGTGTTTGTGACTGAGGACGGCGCAGAAACAGACCTTGATCTTGGCCACTATGAGCGTTTCATC
CGTACTAAGATGAACCGTCGCAACAACTTCACTACGGGTCGTATTTACGAGGAAGTACTGCGTAAAGAGCGTCGCGGTGA
CTATTTAGGCGCGACGATTCAGGTGATCCCACACATCACTAACGCGATCAAAGAAAAAGTGATTGCAGGCGGTGAAGGTC
ACGATGTGGCTATCGTTGAAATCGGCGGTACTGTTGGTGATATCGAATCACTGCCATTCCTTGAGTCTATCCGCCAATTA
GGCGTTGAACTGGGCCGCGATCGTACCCTGTTTATGCATTTAACCTTAGTGCCATTCCTTGGCGCCGCGGGTGAAGTGAA
AACGAAACCGACTCAACATTCAGTGAAAGAATTGCGTTCTATCGGTATTGCACCGGATGTGCTGATCTGCCGTGGCGACC
GCGCGATTCCTGCCAACGAACGTGCGAAGATTTCGTTATTCTGTAACGTTGAAGAACGTGCGGTTATTTCATTAAAAGAC
GTCGACAGCATCTACAAGATCCCTGCGCTGCTGCGTTCACAGGGTTTAGATGACTTAGTGGTTAAGCGTTTTGGCTTAGA
ATGCCGCGAAGCTGACTTGTCTGAGTGGGAAAACGTCATTTACCAAGAAGCCAATCCAAACGGCGAAGTCGTGATTGGTA
TGGTCGGTAAATACATCGAACTGCCAGACGCCTATAAGTCAGTCAACGAAGCATTAAAACACGCGGGTCTGAAGAACCGT
GTATCTGTGAGCATCAAGTATATTGACTCACAAACCGTTGAAGCGAAAGGCGATGAAGTTCTGCAAGGTTTAGACGGTAT
CTTAGTTCCAGGTGGCTTCGGTGAGCGCGGTGTGGAAGGTAAGATCCTAGCGGCTAAATATGCCCGTGAAAACGAGCTGC
CGTACTTTGGTATCTGTTTAGGTATGCAAGTGGCGCTGATTGAATTCGCTCGTAACGTTGCTGGCATGACCGATGCTCAC
TCAACCGAGTTCAATAAAGCAACGCCATTCCCTGTGGTTGGCTTAATCACTGAATGGGTTGATGAAGAAGGTAACGTTGA
ACAACGCCATGAAGCTTCTGATTTAGGTGGCACTATGCGTTTAGGTGCGCAGTTATGTCACCTGCTGGAAGGCTCAAAAG
CGGCGCAAGCGTACAAAGGTAACACTTGTGTTGAGCGTCACCGTCACCGTTACGAAGTGAACAACAAGTACAGAGAACGT
TTAGAGCAAGCGGGTCTGGTATTTAGTGGTTTATCTTCGGACCGTAAACTGGTTGAGATGATTGAGCTGAAAGATCATCC
TTGGTTTGTAGCGGGTCAATTCCACCCAGAATTCACTTCGACCCCACGCGATGGTCATCCATTGTTCGAAGGTTTCGTTG
CTGCCGCAAGTGCACATCAAAAACGTGATCTGAAGAAATAA

Upstream 100 bases:

>100_bases
ATTGGTGTTTGTCGAATGGCAACACCTTTGGTATACTACTGCCCCGTCCTAGTGCTTTCTTACAAGCACGTATTTTCAAA
CTCATTTTCTCAGGTTCAGC

Downstream 100 bases:

>100_bases
ATTTTAAGGCCGGACCCGCGACAGTGGGCCCGGCCTTTTGTTACACTCAGGCCGCGTAATCATGAGTCAAAACTCATACC
CATGTGTATAAACGACATGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVIAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
VSVSIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGMTDAH
STEFNKATPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNTCVERHRHRYEVNNKYRER
LEQAGLVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAASAHQKRDLKK

Sequences:

>Translated_546_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVIAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
VSVSIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGMTDAH
STEFNKATPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNTCVERHRHRYEVNNKYRER
LEQAGLVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAASAHQKRDLKK
>Mature_545_residues
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVIAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLG
VELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKDV
DSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIELPDAYKSVNEALKHAGLKNRV
SVSIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEGKILAAKYARENELPYFGICLGMQVALIEFARNVAGMTDAHS
TEFNKATPFPVVGLITEWVDEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNTCVERHRHRYEVNNKYRERL
EQAGLVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAASAHQKRDLKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=567, Percent_Identity=43.9153439153439, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI28559085, Length=567, Percent_Identity=43.3862433862434, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=567, Percent_Identity=43.3862433862434, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=567, Percent_Identity=43.3862433862434, Blast_Score=463, Evalue=1e-130,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=77.7981651376147, Blast_Score=855, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=38.6252045826514, Blast_Score=420, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=43.2337434094903, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.7543859649123, Blast_Score=445, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=44.7037701974865, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=43.3399602385686, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60223; Mature: 60091

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVIAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHHHHHHHHEECCCCCCCEEEEECCCHHC
LPDAYKSVNEALKHAGLKNRVSVSIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
KILAAKYARENELPYFGICLGMQVALIEFARNVAGMTDAHSTEFNKATPFPVVGLITEWV
CEEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNTCVERHRHRYEVNNKYRER
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHH
LEQAGLVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAASAHQ
HHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLKK
HHCCCC
>Mature Secondary Structure 
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVIAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLICRGDRAIPANERAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLRSQGLDDLVVKRFGLECREADLSEWENVIYQEANPNGEVVIGMVGKYIE
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHHHHHHHHEECCCCCCCEEEEECCCHHC
LPDAYKSVNEALKHAGLKNRVSVSIKYIDSQTVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCC
KILAAKYARENELPYFGICLGMQVALIEFARNVAGMTDAHSTEFNKATPFPVVGLITEWV
CEEEEEHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
DEEGNVEQRHEASDLGGTMRLGAQLCHLLEGSKAAQAYKGNTCVERHRHRYEVNNKYRER
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHH
LEQAGLVFSGLSSDRKLVEMIELKDHPWFVAGQFHPEFTSTPRDGHPLFEGFVAAASAHQ
HHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLKK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA