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The map label for this gene is ycdW [C]

Identifier: 117919036

GI number: 117919036

Start: 683607

End: 684539

Strand: Reverse

Name: ycdW [C]

Synonym: Shewana3_0583

Alternate gene names: 117919036

Gene position: 684539-683607 (Counterclockwise)

Preceding gene: 117919038

Following gene: 117919034

Centisome position: 13.77

GC content: 51.66

Gene sequence:

>933_bases
ATGGGGCACAAGTTACTCTTACTGACCAAGGCGAATGAGCAGTATCGCCAGCTCATTGAGGCGCAGCAGTTGCCAGGGCT
TGAGTTGCTCGATGATAATCCCGCGAATATTGCAGAGGCTAACATTTGGCTTGCCGAACCTAAGCTTGCCGCACCACTGG
TGCCCCATGCCAAACAGCTAAAATGGCTGCAATCCAGTTTTGCAGGCATTGATGCCCTGATGGGCCCAAGAGCGAGAAAA
GATTATCAACTCACTAACATTAAAGGTATTTTCGGCCCCTTGATGAGTGAGTATCTATTTGGTTATCTGCTCGCCCATGT
GCGAGGACATCAGTTTTATCAAGCGCAGCAAAGGCAAAAATCTTGGCAAGTGCAAAGTGCTACGCGCGGCAGCTTGCAAG
GGATGCGATTATTACTCTTAGGCACAGGCTCTATCGCCCAGCACGTGGCCAAGACAGCTAAGCACTTCGGCATGCATATT
ACTGGAATAAACCGCAGCGGCCGTGAGGTCGACGGCTTTGATTTGATCCAGCCGCTCTCGCAGCTCGGGCAATGCTTAAG
CCAAAGCGATGTGGTAACCAATTTACTGCCAAGCACCCCTGAGACACGTTTGCTGCTAAATGCAGACATGCTCGCCACGC
TCAAAGCCGATGCCATTTTAGTGAATGTGGGCCGTGGTGATGTGCTCGACCTCGATGCCCTCAATACCCAGTTGATTGCG
CGTCCTGAACAACATGCCGTTCTCGATGTGTTCACGCAGGAGCCACTCCCTGCGAGTCATCCGATTTGGGAAAGACCCAA
CGCCATCATCACGCCCCATATCTCCGCGCCGAGTCATCCAGAACAAATCGTGCGTATTTTTAGCGACAACTATCGCCGCT
ATATTGCAGCCGAGCCGCTGCAAAATCAGGTCGATTTTATTCAGGGCTACTAG

Upstream 100 bases:

>100_bases
GATCAAACATAATCACTCAACCTAAAATGACGCTGCAAAATCAAAACAACTACAGCAGCGTATTGAAAAATAACGACAAC
CAAGCATAAAGGGAGACGAC

Downstream 100 bases:

>100_bases
TTGGGATGAATAGTTAAAGATAACGAATCCCTACGGTCACTCAGGCAAAAACAAAAGGCAATCAGAGTACTGATTGCCTT
TCGATTAAGCCACTATCCTA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NADPH; glyoxylate; glycerate [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQLKWLQSSFAGIDALMGPRARK
DYQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQKSWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHI
TGINRSGREVDGFDLIQPLSQLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIA
RPEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPLQNQVDFIQGY

Sequences:

>Translated_310_residues
MGHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQLKWLQSSFAGIDALMGPRARK
DYQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQKSWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHI
TGINRSGREVDGFDLIQPLSQLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIA
RPEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPLQNQVDFIQGY
>Mature_309_residues
GHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQLKWLQSSFAGIDALMGPRARKD
YQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQKSWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHIT
GINRSGREVDGFDLIQPLSQLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIAR
PEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPLQNQVDFIQGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=144, Percent_Identity=32.6388888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI23308577, Length=268, Percent_Identity=27.2388059701493, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87081824, Length=220, Percent_Identity=30.9090909090909, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI87082289, Length=178, Percent_Identity=34.2696629213483, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789279, Length=193, Percent_Identity=28.4974093264249, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=264, Percent_Identity=26.5151515151515, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=306, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322116, Length=251, Percent_Identity=23.5059760956175, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571528, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574286, Length=280, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585514, Length=326, Percent_Identity=25.1533742331288, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574282, Length=326, Percent_Identity=25.1533742331288, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574284, Length=326, Percent_Identity=25.1533742331288, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551003, Length=326, Percent_Identity=25.1533742331288, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552429, Length=326, Percent_Identity=25.1533742331288, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921140, Length=264, Percent_Identity=26.8939393939394, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34505; Mature: 34374

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQL
CCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCEEECEEEEECCCCCCCCCCHHHHH
KWLQSSFAGIDALMGPRARKDYQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQK
HHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHITGINRSGREVDGFDLIQPLS
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHH
QLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIA
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHEECCEEEEECCCCCEEEHHHHCHHHHC
RPEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPL
CCCHHHHEEHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCH
QNQVDFIQGY
HHHHHHHCCC
>Mature Secondary Structure 
GHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQL
CCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCEEECEEEEECCCCCCCCCCHHHHH
KWLQSSFAGIDALMGPRARKDYQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQK
HHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHITGINRSGREVDGFDLIQPLS
HHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHH
QLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIA
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHEECCEEEEECCCCCEEEHHHHCHHHHC
RPEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPL
CCCHHHHEEHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCH
QNQVDFIQGY
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycolate; NADP; hydroxypyruvate; NADPH [C]

Specific reaction: glycolate + NADP = NADPH + glyoxylate hydroxypyruvate + NADPH = glycerate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]