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The map label for this gene is glnA [H]

Identifier: 117918757

GI number: 117918757

Start: 334470

End: 335879

Strand: Reverse

Name: glnA [H]

Synonym: Shewana3_0300

Alternate gene names: 117918757

Gene position: 335879-334470 (Counterclockwise)

Preceding gene: 117918759

Following gene: 117918756

Centisome position: 6.76

GC content: 47.87

Gene sequence:

>1410_bases
ATGTCAGTTGAATCAGTTTTAAAGCAACTTGAAGAATTAGAAGTTAAGTTTGTTGATTTACGTTTTACCGACACCAAAGG
TAAAGAGCAGCACGTATCTATTCCTGCTCATCAGGTAGACGCTGATTTCTTCGAAGACGGCAAAATGTTTGACGGTTCGT
CAATCGCTGGCTGGAAAGGCATTAACGAATCAGACATGGTGCTGATGCCAGACCCAACCACGTTTGTATTAGACCCATTC
ACCGAAGAAACCACTGCACTGATCCGCTGTGACATTCTCGAGCCAGGCACTATGACTGGTTATGACCGTGACCCACGTTC
TATCGCGAAAAAAGCGGAAGAATACTTACGCTCTACCGGTATCGCTGACACTGTATTAGTTGGACCAGAACCAGAATTCT
TCCTGTTCGACGACGTACGTTTCGGTACTGACATGTCTGGTTGTTTCGTGAAAATCGACGCTAAAGAAGCGGCATGGAAC
TCTGGCACTAGCTACGAAGGTGGTAACACTGGTCACCGTCCATTCGTGAAAGGCGGTTACTTCCCAGTAGCTCCTGTTGA
CTCTTCACAAGACTTACGTAGCGCTATGTGTTTAGTGCTCGAAGAAATGGGTCAAGTAGTTGAAGCTCACCACCACGAAG
TGGCGACTGCGGGTCAAAACGAAATCGCAACTCGCTTCAACACTCTGACTAAGAAAGCGGACGAAATCCAAATTCTGAAG
TACGTAGTACACAACATGGCTCACGCCTACGGTAAGACTGCGACTTTCATGCCTAAGCCAATCGTTGGCGACAACGGTAG
CGGTATGCACGTTCACCAATCTCTGTCTAAAGACGGTGTGAACCTGTTCGCTGGCGACAAATACGCTGGTTTAAGTGAAA
CTGCACTGTACTACATCGGTGGTATCATCAAGCACGCTCGCGCACTGAACGCCTTCACTAACCCAAGCACTAACTCTTAC
AAGCGTTTAGTACCACACTTTGAAGCGCCAGTGATGTTAGCTTACTCAGCACGTAACCGCAGCGCTTCAATCCGTATTCC
AGTGGTACCAAGCCCTAAAGGCCGTCGTATCGAAACTCGCTTCCCAGATCCACATGCAAACCCATACTTAGGTTTCGCAG
CGCTGCTGATGGCGGGTATCGACGGTATCCAAAACAAGATCCATCCAGGCGAAGCTATGGATAAAGACTTGTACGATCTG
CCAGCGGAAGAAGCGGCTGAAATCCCACAAGTAGCGACTTCATTAGAAAACGCACTTGAGAACCTGCAAGCTGACCACGA
GTTCTTAACTCGCGGCGGTGTATTCAGCGAAGATTTCATCCAATCTTACATTGCACTGAAATCTGCAGAAGCTGAGCGTG
TAAGCCGTACAACTCACCCATTAGAGTTCGAGATGTACTACAGCCTGTAA

Upstream 100 bases:

>100_bases
GGCATAGATATCGCTATATAGCTTGTAAGTTTGGCTCAAGCACGACTTGAGTTAAAAGATTATAGAAGGGACATCCCTTT
ACCCTACCGGAGACTTGAGA

Downstream 100 bases:

>100_bases
TGGCTAACGGCATCGATTAGCTGCACAACATCTAAAAGCCCCGACAGTCTTCTGTCGGGGCTTTTTTGTCTCTATCGTTC
TGTGACTCCTAATTATTCCC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPAHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPF
TEETTALIRCDILEPGTMTGYDRDPRSIAKKAEEYLRSTGIADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWN
SGTSYEGGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDL
PAEEAAEIPQVATSLENALENLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVSRTTHPLEFEMYYSL

Sequences:

>Translated_469_residues
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPAHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPF
TEETTALIRCDILEPGTMTGYDRDPRSIAKKAEEYLRSTGIADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWN
SGTSYEGGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDL
PAEEAAEIPQVATSLENALENLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVSRTTHPLEFEMYYSL
>Mature_468_residues
SVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPAHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPFT
EETTALIRCDILEPGTMTGYDRDPRSIAKKAEEYLRSTGIADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNS
GTSYEGGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILKY
VVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSYK
RLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDLP
AEEAAEIPQVATSLENALENLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVSRTTHPLEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=463, Percent_Identity=26.133909287257, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=78.0383795309168, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=368, Percent_Identity=26.9021739130435, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51788; Mature: 51657

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPAHQVDADFFEDGKMFDGSSIAGWKG
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCHHHCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPTTFVLDPFTEETTALIRCDILEPGTMTGYDRDPRSIAKKAEEYLRSTG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNSGTSYEGGNTGHRPFVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECC
FPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDLPAEEAAEIPQVATSLENALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVSRTTHPLEFEMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPAHQVDADFFEDGKMFDGSSIAGWKG
CHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCHHHCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPTTFVLDPFTEETTALIRCDILEPGTMTGYDRDPRSIAKKAEEYLRSTG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNSGTSYEGGNTGHRPFVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLSKDGVNLFAGDKYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECC
FPDPHANPYLGFAALLMAGIDGIQNKIHPGEAMDKDLYDLPAEEAAEIPQVATSLENALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLQADHEFLTRGGVFSEDFIQSYIALKSAEAERVSRTTHPLEFEMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]