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The map label for this gene is dcuB [H]

Identifier: 117918750

GI number: 117918750

Start: 325290

End: 326633

Strand: Reverse

Name: dcuB [H]

Synonym: Shewana3_0293

Alternate gene names: 117918750

Gene position: 326633-325290 (Counterclockwise)

Preceding gene: 117918753

Following gene: 117918748

Centisome position: 6.57

GC content: 50.74

Gene sequence:

>1344_bases
ATGATTTTAATGGAGTTTATTGTCGTACTCGGCTGTTTACTGCTCGGTACCCGTTACGGTGGTATGGGGCTCGGGTTGAT
AAGTGGTATTGGCTTATTTTTACTGACATTTGTGTTTGGTCTCGCCCCCGGACAACCGCCAGTACAAGTGATGCTCACCA
TTCTTGCGGTGATCGGCTGCGCCTCAGTACTGCAAACCGCAGGCGGTTTAAATGTGATGATGCAGTTTGCCGAGCGTTTA
CTGCGCCGCCACCCACAATACATCACCATTCTGGCGCCGCTAACCACTTGGACCTTAACCTTCTTATGCGGAACTGGCCA
CGTGGTTTACACCATGTTCCCGATCATCTCCGACATCGCCCTGAAGAAAAATATTCGTCCAGAACGCCCGATGGCCGTGG
CCTCGGTCGCCTCGCAAATGGCGATTTGCGCCTCTCCGGTGTCGGTCGCAGTGGTTTCTATGGTATCGATTTTGGCGGCG
CAGCACGGCGTTGGTCATGCCTATAGCATGCTGGAAATTTTAATGGTGTCCGTACCAGCATCCCTTTGCGGCGTGTTAGT
GGCGGCACTCTGGAGCCTGCGTCGTGGTAAAGATCTAGATAAGGACGAAGAGTTTCAGGCGCGAATTAAAGATCCCGAAC
AAAGGGCATTTATCTACGACAAGAGCGAGACTCTGCTGGAACAAACCTTTCCTAAGGAAGCCTATTGGGCAACGGGGATT
TTCTTTACCGCTATCGCCGCCGTGGTGTTACTCGGTTCCTTTAGCGAACTGCGGCCTGTGTTTGAGGTAAAGGGCAAGTT
GGCGCCGCTGTCGATGAACTTAGTGATCCAGATGATGATGTTGATCGCTGGGGCTTTTATCTTAATGAGCTGCAAAGTCA
AACCCACAGAAATTGCCAATGGCCCAGTGTTTAAAGCTGGCATGGTGGCGATTTTCTCGGTATTTGGCGTGGCTTGGATG
AGTGATACTTACTTTATCAGCCATATGGATGTGCTGAAGGAAAACCTCTCCCATGTGGTGCAAAACCAACCTTGGACCTA
TGCCTTAGTGCTGTTTTTAATCTCAAAACTGGTCAACAGCCAAGCGGCGGCGCTCACGGCTATCGCGCCCATGGGCTTGG
CTTTAGGCGTTGATCCTAAGCTGTTGATTGCCTTTTTACCCGCAAGCTATGGTTACTTTGTACTACCTACTTATCCAAGC
GATTTGGCCTGCATCGGCTTCGACCGAACTGGCACCACCAAAATTGGTAAGTTCATCATTAACCACAGCTTTATTATTCC
GGGATTGATCGGTGTGGGTACGGCGTCGACCGTCGGTTATCTGCTGGCGACCACCTTACTGTAA

Upstream 100 bases:

>100_bases
CGCCAGCGCAAGTTCTCAAATCCATTCCCCTCGCACAATAGCGCCAAATACAGCTAGTTCTAATGCAAATCGCGCGGCAG
GTTCCGCAGGGGTGAATCAA

Downstream 100 bases:

>100_bases
GCACGGTTTGAAGCGATAAAACACATAAGGCCGATAATGTGAAAGTATTATCGGCCTTATGATTGATACGAAAGAAAAAA
CTAAGCGCGATACATGGCGA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MILMEFIVVLGCLLLGTRYGGMGLGLISGIGLFLLTFVFGLAPGQPPVQVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
QHGVGHAYSMLEILMVSVPASLCGVLVAALWSLRRGKDLDKDEEFQARIKDPEQRAFIYDKSETLLEQTFPKEAYWATGI
FFTAIAAVVLLGSFSELRPVFEVKGKLAPLSMNLVIQMMMLIAGAFILMSCKVKPTEIANGPVFKAGMVAIFSVFGVAWM
SDTYFISHMDVLKENLSHVVQNQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRTGTTKIGKFIINHSFIIPGLIGVGTASTVGYLLATTLL

Sequences:

>Translated_447_residues
MILMEFIVVLGCLLLGTRYGGMGLGLISGIGLFLLTFVFGLAPGQPPVQVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
QHGVGHAYSMLEILMVSVPASLCGVLVAALWSLRRGKDLDKDEEFQARIKDPEQRAFIYDKSETLLEQTFPKEAYWATGI
FFTAIAAVVLLGSFSELRPVFEVKGKLAPLSMNLVIQMMMLIAGAFILMSCKVKPTEIANGPVFKAGMVAIFSVFGVAWM
SDTYFISHMDVLKENLSHVVQNQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRTGTTKIGKFIINHSFIIPGLIGVGTASTVGYLLATTLL
>Mature_447_residues
MILMEFIVVLGCLLLGTRYGGMGLGLISGIGLFLLTFVFGLAPGQPPVQVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
QHGVGHAYSMLEILMVSVPASLCGVLVAALWSLRRGKDLDKDEEFQARIKDPEQRAFIYDKSETLLEQTFPKEAYWATGI
FFTAIAAVVLLGSFSELRPVFEVKGKLAPLSMNLVIQMMMLIAGAFILMSCKVKPTEIANGPVFKAGMVAIFSVFGVAWM
SDTYFISHMDVLKENLSHVVQNQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRTGTTKIGKFIINHSFIIPGLIGVGTASTVGYLLATTLL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=407, Percent_Identity=55.036855036855, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1790580, Length=451, Percent_Identity=37.6940133037694, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 48213; Mature: 48213

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMEFIVVLGCLLLGTRYGGMGLGLISGIGLFLLTFVFGLAPGQPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ASVLQTAGGLNVMMQFAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAAQHGVGHAYSMLEILMVSVPA
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
SLCGVLVAALWSLRRGKDLDKDEEFQARIKDPEQRAFIYDKSETLLEQTFPKEAYWATGI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHEEEECCHHHHHHHHCCCHHHHHHHH
FFTAIAAVVLLGSFSELRPVFEVKGKLAPLSMNLVIQMMMLIAGAFILMSCKVKPTEIAN
HHHHHHHHHHHCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPVFKAGMVAIFSVFGVAWMSDTYFISHMDVLKENLSHVVQNQPWTYALVLFLISKLVNS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPSDLACIGFDRTGTTKIGKFII
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVGTASTVGYLLATTLL
CCCHHHCCHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MILMEFIVVLGCLLLGTRYGGMGLGLISGIGLFLLTFVFGLAPGQPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ASVLQTAGGLNVMMQFAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAAQHGVGHAYSMLEILMVSVPA
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
SLCGVLVAALWSLRRGKDLDKDEEFQARIKDPEQRAFIYDKSETLLEQTFPKEAYWATGI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHEEEECCHHHHHHHHCCCHHHHHHHH
FFTAIAAVVLLGSFSELRPVFEVKGKLAPLSMNLVIQMMMLIAGAFILMSCKVKPTEIAN
HHHHHHHHHHHCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPVFKAGMVAIFSVFGVAWMSDTYFISHMDVLKENLSHVVQNQPWTYALVLFLISKLVNS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVDPKLLIAFLPASYGYFVLPTYPSDLACIGFDRTGTTKIGKFII
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVGTASTVGYLLATTLL
CCCHHHCCHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]