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The map label for this gene is gsp [C]

Identifier: 117918747

GI number: 117918747

Start: 322495

End: 323184

Strand: Reverse

Name: gsp [C]

Synonym: Shewana3_0290

Alternate gene names: 117918747

Gene position: 323184-322495 (Counterclockwise)

Preceding gene: 117918748

Following gene: 117918746

Centisome position: 6.5

GC content: 48.7

Gene sequence:

>690_bases
ATGGTCAAAGGTTTTCGTTTATTGCTACTCACAGTAAGCCTAGTCTGCGGTCTTGGTTATCTCGGCTACCACGGGCTGAC
TCGTTATAACTTAAATACGGACTATCAAGTGAGTGATGTGCTCGACTCACTCAATGGCGTCGAGATTTATTATAACGGCG
GCGTCAACACTAACGAGGGGCGCAACCTCTCCCTCGACGGCTATAATCTGGGTATCAAATACCAATGCGTAGAGTTCGTT
AAACGTTACTTTTACCTGCGTTATCAGCACAAAATGCCCGACAGTTTCGGTCACGCCAAAGACTTCTTCAATGACCTTTT
GCCCGATGCGAGCTGGAATGAAAAACGCGCCCTACGGCAATATACCAATGGCAGCCAAAGCAAGCCGATGGCTGATGATT
TACTGGTATTCGCGCCATGGGTGTTTAATCCCTACGGCCATGTCGCCATTATCGCCAACGTCACAGAAACCAGTATCGAA
ATTGCCCAGCAAAATCCCGGCCCTTTTGCACCGTCACGGGAGGTTTTTCCCTTAGTGCAACGTGAAGGCCTGTGGTATAT
CGATGCCCCTCGCACTAAGGGCTGGTTACGCTGGGAAGCTCCAGCTGTCACGATGATGAAGGGAAGTGCTGAGGATAATG
TGGAGCCAAATATCCAAGTTAACCAACAAGCTATTCTCCTTAAACTGTGA

Upstream 100 bases:

>100_bases
CAAACCGCCAGTACGCCTAGACGTTTTTAGCGACAACAGCATACAATCCGACCTTGTATCCTGCTGAATCAATTTATCTT
TTGAGAATGTCGTACTAAAG

Downstream 100 bases:

>100_bases
ATGTCGTAGAGGTAAAAATGAGACTACTCGACTGGCTAAAACGACACCTTGGCCCTAAGGCAAATACGGACTCACAGACT
ACGGATCTTCAAGCAATTTT

Product: CHAP domain-containing protein

Products: ADP; Glutathionylspermidine; H+; Phosphate [C]

Alternate protein names: Trypanothione Synthetase Domain Protein; Amidase; Chap Domain Containing Protein; Trypanothione Synthetase Domain-Containing Protein

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MVKGFRLLLLTVSLVCGLGYLGYHGLTRYNLNTDYQVSDVLDSLNGVEIYYNGGVNTNEGRNLSLDGYNLGIKYQCVEFV
KRYFYLRYQHKMPDSFGHAKDFFNDLLPDASWNEKRALRQYTNGSQSKPMADDLLVFAPWVFNPYGHVAIIANVTETSIE
IAQQNPGPFAPSREVFPLVQREGLWYIDAPRTKGWLRWEAPAVTMMKGSAEDNVEPNIQVNQQAILLKL

Sequences:

>Translated_229_residues
MVKGFRLLLLTVSLVCGLGYLGYHGLTRYNLNTDYQVSDVLDSLNGVEIYYNGGVNTNEGRNLSLDGYNLGIKYQCVEFV
KRYFYLRYQHKMPDSFGHAKDFFNDLLPDASWNEKRALRQYTNGSQSKPMADDLLVFAPWVFNPYGHVAIIANVTETSIE
IAQQNPGPFAPSREVFPLVQREGLWYIDAPRTKGWLRWEAPAVTMMKGSAEDNVEPNIQVNQQAILLKL
>Mature_229_residues
MVKGFRLLLLTVSLVCGLGYLGYHGLTRYNLNTDYQVSDVLDSLNGVEIYYNGGVNTNEGRNLSLDGYNLGIKYQCVEFV
KRYFYLRYQHKMPDSFGHAKDFFNDLLPDASWNEKRALRQYTNGSQSKPMADDLLVFAPWVFNPYGHVAIIANVTETSIE
IAQQNPGPFAPSREVFPLVQREGLWYIDAPRTKGWLRWEAPAVTMMKGSAEDNVEPNIQVNQQAILLKL

Specific function: Catalyzes The Formation Of An Amide Bond Between Glutathione And Spermidine Coupled With Hydrolysis Of ATP; Also Catalyzes The Hydrolysis Of Glutathionylspermidine To Glutathione And Spermidine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.1.8; 3.5.1.78 [C]

Molecular weight: Translated: 26067; Mature: 26067

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGFRLLLLTVSLVCGLGYLGYHGLTRYNLNTDYQVSDVLDSLNGVEIYYNGGVNTNEG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCC
RNLSLDGYNLGIKYQCVEFVKRYFYLRYQHKMPDSFGHAKDFFNDLLPDASWNEKRALRQ
CEEEECCEECCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
YTNGSQSKPMADDLLVFAPWVFNPYGHVAIIANVTETSIEIAQQNPGPFAPSREVFPLVQ
HCCCCCCCCCCCCEEEECCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHH
REGLWYIDAPRTKGWLRWEAPAVTMMKGSAEDNVEPNIQVNQQAILLKL
HCCEEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCEEECCEEEEEEC
>Mature Secondary Structure
MVKGFRLLLLTVSLVCGLGYLGYHGLTRYNLNTDYQVSDVLDSLNGVEIYYNGGVNTNEG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCC
RNLSLDGYNLGIKYQCVEFVKRYFYLRYQHKMPDSFGHAKDFFNDLLPDASWNEKRALRQ
CEEEECCEECCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
YTNGSQSKPMADDLLVFAPWVFNPYGHVAIIANVTETSIEIAQQNPGPFAPSREVFPLVQ
HCCCCCCCCCCCCEEEECCHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHH
REGLWYIDAPRTKGWLRWEAPAVTMMKGSAEDNVEPNIQVNQQAILLKL
HCCEEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Reduced glutathione; Spermidine [C]

Specific reaction: ATP + Reduced glutathione + Spermidine --> ADP + Glutathionylspermidine + H+ + Phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA