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The map label for this gene is ycjM [C]

Identifier: 117918525

GI number: 117918525

Start: 76921

End: 78405

Strand: Reverse

Name: ycjM [C]

Synonym: Shewana3_0066

Alternate gene names: 117918525

Gene position: 78405-76921 (Counterclockwise)

Preceding gene: 117918530

Following gene: 117918520

Centisome position: 1.58

GC content: 47.14

Gene sequence:

>1485_bases
ATGAAAAATCAAGTGCAATTGATCACCTATGTCGATCGGATAACCGGTGCCGGACTCAAACAACTCAAAGCCTTACTCGA
TAATGAGTTAGATGGTTTATTTGGGGGCGTTCATCTACTGCCATTTTACTATCCCATCGATGGCAGCGATGCAGGGTTTG
ATCCCATAGACCATGTACAAGTGGACTCACGCTTAGGTGATTGGGACGATGTCAAAAGCATAGGCGAAGCGTTCGACATC
ATGGCCGACTTGATCGTTAACCATATGTCCGCAGAATCTCCCGAGTTTAAAGATGTGCTCAAACATGGCAAACAATCGGT
TTACTGGGATTTATTCCTGACCAAGGACAAGGTATTCCCAGAGGGATTAAGTGAACAGGATCAAAAAGCTATCTATCGCC
CCCGCCCAGGAAGCTGCTTTAGCACTTACACCTTAGCGGATGGTTCAACGGAAGAATTTTGGACCACCTTTACCCGTAAC
CAGATCGATATCGATGTTAACTCAGCTGCAGGTAAGGATTATTTAAACCGCGTATTGGCGCGATTTAACCACAGCAAAGT
CAATTTAATCCGCTTAGATGCCGCAGGTTATGCGATAAAAAAACCCGGCACTAGCTGCTTTATGATTGAGGAATCCTTCG
AGTTTGTGGATAAATTAGCGAAACAGGCCAATGAGTTAGGTATGACAACCCTAGCCGAAATCCACTCCCATCATATGACG
CAAATCGATATCGCCAAACGAGTGGACATGGTTTACGACTTCGCCCTGCCACCGCTCATCCTGCACACGCTGTTTAGCCA
AGATTGTAGCGCCCTGAGTCACTGGCTAGACATGGCCCCTCGCAACTGCATTACCGTTCTCGATACCCACGATGGCATTG
GTATTATCGATGTCGGCCCAATGGATGGCTTACGCGGATTATTGAACGAAAAACAAATCGATAATCTGGTCGAAACCATA
CACGGCAACAGTCAAGGCGAGAGTCTACAAGCCACTGGGGCGGCCGCCAGCAATGTCGATTTGTATCAAATCAACTGCAC
CTATTACGATGCCCTAGGGCAAAATGATCTAGATTACTTGATGGCGCGAGCAATTCAGTTTTTCGCACCGGGTATTCCTC
AGGTCTACTACACAGGCCTGCTTGCCATACCAAACGACATGACGTTGCTGAATCAAACCCACGTCGGCCGAGACATTAAT
CGCCCCTATTTAAACCGGGAAAAAGTGCAACAAGCCCTGCAAAAACCTGTGGTTAAAGCCCTCATCCAATTGATTAAACT
GCGTAATAATAGCGCAGCATTCAATGGCCGCTTTTCGATGGCTGCAAGTGGAAAAACACTAACTCTCACATGGTCTGCAC
AAAATGCATCGGCCAGTCTTAGTCTCGACTTTGCCCATAAACAAGGGCTGATCACCCTACTTGATGAGCAAGGCATCGAA
ACCCAAATCGCATTAGCAGACCTGCTCACTCAAGCACAGGCCTAA

Upstream 100 bases:

>100_bases
GGTTAAATATACAACTTAATCGATTAAGTTTGTTTGTGCAATTTATAAATAACCGCCAGCAAACTTTACTCTGGGAAGGC
TACTAAAGCGTGGGCAAAGT

Downstream 100 bases:

>100_bases
TGCAGAACAAAAACAAAAGGCGCTTAATTAGCGCCTTTTGCATATAAAACTGATTGTATTAACAGCTTTTGCCTAAACGT
AGTTCATACCCTAATACCTG

Product: sucrose phosphorylase

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQVDSRLGDWDDVKSIGEAFDI
MADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFPEGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRN
QIDIDVNSAAGKDYLNRVLARFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGPMDGLRGLLNEKQIDNLVETI
HGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYLMARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDIN
RPYLNREKVQQALQKPVVKALIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
TQIALADLLTQAQA

Sequences:

>Translated_494_residues
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQVDSRLGDWDDVKSIGEAFDI
MADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFPEGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRN
QIDIDVNSAAGKDYLNRVLARFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGPMDGLRGLLNEKQIDNLVETI
HGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYLMARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDIN
RPYLNREKVQQALQKPVVKALIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
TQIALADLLTQAQA
>Mature_494_residues
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQVDSRLGDWDDVKSIGEAFDI
MADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFPEGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRN
QIDIDVNSAAGKDYLNRVLARFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGPMDGLRGLLNEKQIDNLVETI
HGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYLMARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDIN
RPYLNREKVQQALQKPVVKALIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
TQIALADLLTQAQA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=461, Percent_Identity=31.4533622559653, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377
- InterPro:   IPR022527 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 54840; Mature: 54840

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQ
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
VDSRLGDWDDVKSIGEAFDIMADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCCCC
EGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRNQIDIDVNSAAGKDYLNRVLA
CCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
RFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
HCCCCCEEEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
MDGLRGLLNEKQIDNLVETIHGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCCCHHHH
MARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDINRPYLNREKVQQALQKPVVKA
HHHHHHHHCCCCCHHHHCCEEECCCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
HHHHHHHCCCCCEECCEEEEEECCCEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCCH
TQIALADLLTQAQA
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQ
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEE
VDSRLGDWDDVKSIGEAFDIMADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEEECCCCCC
EGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRNQIDIDVNSAAGKDYLNRVLA
CCCCCHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
RFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
HCCCCCEEEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
MDGLRGLLNEKQIDNLVETIHGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCCCHHHH
MARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDINRPYLNREKVQQALQKPVVKA
HHHHHHHHCCCCCHHHHCCEEECCCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
HHHHHHHCCCCCEECCEEEEEECCCEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCCH
TQIALADLLTQAQA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8012038 [H]