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The map label for this gene is rsmB [H]

Identifier: 117918492

GI number: 117918492

Start: 40083

End: 41372

Strand: Reverse

Name: rsmB [H]

Synonym: Shewana3_0033

Alternate gene names: 117918492

Gene position: 41372-40083 (Counterclockwise)

Preceding gene: 117918493

Following gene: 117918491

Centisome position: 0.83

GC content: 52.4

Gene sequence:

>1290_bases
ATGATGAACTTGCGTGCACTGGCGGCCAAAGCCATCTTCGAAGTCTTAGAGAAAGGCGTATCACTTTCAGTTGCCCTGCC
AGAACAGCAAAAGCATCTTGCCAGTGGTAAAGATAAAGCCCTGCTCGCAGAGCTCTGCTACGGCGTGATGCGCACCTTGC
CGCAGATCGAAAAACGCGTGGCCGAGTGTTTAGCCAAGCCGCTGAAAGGTAAGCAAAGGATCATCCACCAGCTATTAATT
GTTGGCTGTTATCAACTTTACTTCACCCGCATTCCAAGCCACGCCGCCATCTCCGAAACCGCCGAGGCCTGCCGTCAGTT
AAAATTTGAGGGCATGGTAAAGGTGGTCAATGGCGTGCTGCGTAATATTCAGCGCCAATTAACGCCGCTAAGCACTGAGT
CAGAGACCTTAAGCTACAACACGCCGGGCTGGTTAATTAAGCGCTTAAAAGCCGCTTATCCCGATAATTGGCAAGAGATA
ATCCAGCAAAGCCATGAGCGTCCACCAATGTGGCTACGTAACAATCGCCTCTCCCAAAGCCGCGATGCATATCTTGCCGC
CCTGAGTGAACTTGAGATAGAAGCAAGTGCAGGCCTCAGTGATGATGCCATTCTACTTGCCCATCCCAAGGATGTGGCAA
CATTGCCGCGCTTCCACGAAGGTGCGGCCTCGGTGCAAGATGGCGCCGCCCAATGGGCCGCAACCCTGCTGGCTCCGCAA
GCCAACGAACTGGTCCTCGATGCCTGTGCGGCACCGGGCGGCAAAAGCTGCCATTTACTCGAACTTGAGCCGAGCATCCA
ACTGGTCGCCGTCGATTTCGATGCTAAACGACTGGAACGCGTGCAACAGAACCTTGACCGTTTGTCATTAAAAGCGGAAG
TTATTCATGGCGATGCAGCCAACATCGACTCATGGTGGCAGGGTGAACAGTTTGATCGTATCTTACTCGACGCGCCTTGC
TCTGCGACGGGCGTTATCCGCCGTCATCCCGATATCAAGTGGTTACGCAAAAACCATGACATCGAAGAACTGGCCGAGCT
GCAAAGACAAATTCTCGATCACTGCTGGAAGTGGCTCAAGCCCGGTGGCACACTCCTGTATGCAACTTGCTCTATTTTGC
CGCAGGAAAATCGAGACCAAATTAGTGCATTTTTAGCCAGAACCGCAGATGCTAAGCTAGACACTCTTGCCCAGCAGGCT
TCAAGCCAAGATATAGGTTGGCAAATCACGCCGGGACAACACAACATGGACGGGTTTTATTACGCCCGTTTATTGAAAGC
GACCCATTAG

Upstream 100 bases:

>100_bases
TGCAATTACCCGGTAAAAAACCACTCAGCGTGGCCGACATACTCAATGCCCGCGGCGATTGGTTTACCCCAAACACTCGT
CTTAATAACGAGGCGCAATA

Downstream 100 bases:

>100_bases
GAAGTAAGCAAGGCCATGAAAATTATCATATTAGGTGCGGGTCAAGTCGGGGGAACCTTGGCCGAAAATTTGGTGGGTGA
AAATAACGATATCACCATAG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MMNLRALAAKAIFEVLEKGVSLSVALPEQQKHLASGKDKALLAELCYGVMRTLPQIEKRVAECLAKPLKGKQRIIHQLLI
VGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLTPLSTESETLSYNTPGWLIKRLKAAYPDNWQEI
IQQSHERPPMWLRNNRLSQSRDAYLAALSELEIEASAGLSDDAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLAPQ
ANELVLDACAAPGGKSCHLLELEPSIQLVAVDFDAKRLERVQQNLDRLSLKAEVIHGDAANIDSWWQGEQFDRILLDAPC
SATGVIRRHPDIKWLRKNHDIEELAELQRQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLARTADAKLDTLAQQA
SSQDIGWQITPGQHNMDGFYYARLLKATH

Sequences:

>Translated_429_residues
MMNLRALAAKAIFEVLEKGVSLSVALPEQQKHLASGKDKALLAELCYGVMRTLPQIEKRVAECLAKPLKGKQRIIHQLLI
VGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLTPLSTESETLSYNTPGWLIKRLKAAYPDNWQEI
IQQSHERPPMWLRNNRLSQSRDAYLAALSELEIEASAGLSDDAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLAPQ
ANELVLDACAAPGGKSCHLLELEPSIQLVAVDFDAKRLERVQQNLDRLSLKAEVIHGDAANIDSWWQGEQFDRILLDAPC
SATGVIRRHPDIKWLRKNHDIEELAELQRQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLARTADAKLDTLAQQA
SSQDIGWQITPGQHNMDGFYYARLLKATH
>Mature_429_residues
MMNLRALAAKAIFEVLEKGVSLSVALPEQQKHLASGKDKALLAELCYGVMRTLPQIEKRVAECLAKPLKGKQRIIHQLLI
VGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLTPLSTESETLSYNTPGWLIKRLKAAYPDNWQEI
IQQSHERPPMWLRNNRLSQSRDAYLAALSELEIEASAGLSDDAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLAPQ
ANELVLDACAAPGGKSCHLLELEPSIQLVAVDFDAKRLERVQQNLDRLSLKAEVIHGDAANIDSWWQGEQFDRILLDAPC
SATGVIRRHPDIKWLRKNHDIEELAELQRQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLARTADAKLDTLAQQA
SSQDIGWQITPGQHNMDGFYYARLLKATH

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=378, Percent_Identity=28.3068783068783, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI76150623, Length=378, Percent_Identity=28.3068783068783, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI32698918, Length=226, Percent_Identity=30.0884955752212, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI301336155, Length=203, Percent_Identity=30.5418719211823, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI39995082, Length=203, Percent_Identity=30.5418719211823, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316918, Length=176, Percent_Identity=30.6818181818182, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI11545785, Length=171, Percent_Identity=34.5029239766082, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI2367212, Length=427, Percent_Identity=51.9906323185012, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI87081985, Length=205, Percent_Identity=30.2439024390244, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=314, Percent_Identity=27.3885350318471, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25143471, Length=179, Percent_Identity=35.195530726257, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17509941, Length=193, Percent_Identity=29.0155440414508, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71998419, Length=182, Percent_Identity=31.3186813186813, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=182, Percent_Identity=31.3186813186813, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=235, Percent_Identity=30.2127659574468, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=33.1550802139037, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI22024126, Length=319, Percent_Identity=28.5266457680251, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19920494, Length=164, Percent_Identity=34.7560975609756, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356513, Length=186, Percent_Identity=31.7204301075269, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24668781, Length=186, Percent_Identity=31.7204301075269, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355201, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47962; Mature: 47962

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLRALAAKAIFEVLEKGVSLSVALPEQQKHLASGKDKALLAELCYGVMRTLPQIEKRV
CCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AECLAKPLKGKQRIIHQLLIVGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIQRQLTPLSTESETLSYNTPGWLIKRLKAAYPDNWQEIIQQSHERPPMWLRNNRLSQS
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCCCH
RDAYLAALSELEIEASAGLSDDAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLAPQ
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCHHCCCHHHHHHHHHHHHHHHCCC
ANELVLDACAAPGGKSCHLLELEPSIQLVAVDFDAKRLERVQQNLDRLSLKAEVIHGDAA
CCHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCC
NIDSWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRKNHDIEELAELQRQILDHCWKWLK
CCCCCCCCCCCCHHEEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
PGGTLLYATCSILPQENRDQISAFLARTADAKLDTLAQQASSQDIGWQITPGQHNMDGFY
CCCEEEEEEEHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCHHH
YARLLKATH
HHHHHHHCC
>Mature Secondary Structure
MMNLRALAAKAIFEVLEKGVSLSVALPEQQKHLASGKDKALLAELCYGVMRTLPQIEKRV
CCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AECLAKPLKGKQRIIHQLLIVGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIQRQLTPLSTESETLSYNTPGWLIKRLKAAYPDNWQEIIQQSHERPPMWLRNNRLSQS
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCCCH
RDAYLAALSELEIEASAGLSDDAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLAPQ
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCHHCCCHHHHHHHHHHHHHHHCCC
ANELVLDACAAPGGKSCHLLELEPSIQLVAVDFDAKRLERVQQNLDRLSLKAEVIHGDAA
CCHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCC
NIDSWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRKNHDIEELAELQRQILDHCWKWLK
CCCCCCCCCCCCHHEEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
PGGTLLYATCSILPQENRDQISAFLARTADAKLDTLAQQASSQDIGWQITPGQHNMDGFY
CCCEEEEEEEHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCHHH
YARLLKATH
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]