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The map label for this gene is IS1086 [H]

Identifier: 117676276

GI number: 117676276

Start: 226703

End: 227731

Strand: Direct

Name: IS1086 [H]

Synonym: Shewana3_4339

Alternate gene names: 117676276

Gene position: 226703-227731 (Clockwise)

Preceding gene: 117676274

Following gene: 117676277

Centisome position: 81.27

GC content: 64.04

Gene sequence:

>1029_bases
ATGTCCTATCACGAACTCAGTATCCCCGAACGTGCGACCCTTCAACTCGGCCTTGCCCAAGGTTTCAGCCAGCGGCGAAT
TGCCCGGATGCTCGGCCGCTCCCCCTCGACCATCAGCCGGGAGCTGCGCCGCAACCGGGCCCCTGGCACCCGCTACCAGG
CCTGTGCTGCCCAGCAGCAGATGCAGGTCCGCCGCCAGGTCTGCAGGCCTCAGTGCAAGCTGCTGCCGGGCAGTGAGCGC
TTCGAGCTGGTCGTCCATATGCTGCGTGAGGGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACC
CAGCCTCAGAGAGGCCTACGTCTGCCGCGAGACGATCTATAACGCGATCTACGCCCTGCCGGTCGGTGGCCTGCGCAAGG
AGCTGATCATCTGCCTGCGCCAGGGCAAGACGAGTCGCAGGCCGCGCTCTGGCGGCGTCGATCGGCGCGGCCAGATCCCT
GAAATGCTCAGCATCCACGTGCGCCCGCCGGAGGTCGAAGACCGGCTGATGCCAGGCCATTGGGAGGGCGACCTGATCAA
GGGCAAGGCCAATGCCTCGGCGGTGGGCACCTTGGTGGAGCGTACCAGCGGCTACCTGATGCTGATCAAGATGGCCGACG
CGACGGCGACCTCGGCGCTGGAGGGCTTCAGTGCGGCGCTCAATGGCATGCCGCTGGCGGTGCGCAAGAGCATGACCTAC
GACCAGGGCCGGGAGATGGCGCGGCACGCCGAGATCACCCAGCGGACCGGGGTGGCGATCTACTTCTGCGACCCGCACAG
CCCCTGGCAACGCGGCAGCAATGAGAACATCAATGGACTGATCCGCCAGTACCTGCCCAAGGGCACGGACTTGTCGGTGC
ATAGTCAGGAAGCCCTGGACGCCATCGCTTTGCAACTGAATATGCGACCGCGCAAACGCTTCGACTTCAAATGCCCGATC
GAGGTGATGGGCGAAGTGATGCAAGAAGCCGTGGTGATGCGGCATGATGCTCCCGCTTCAATTCAATAA

Upstream 100 bases:

>100_bases
ACCAATATTCTTTCGACTACCTCCCCTCAAAGCCATATCACGCACGGCGGCCGCAAAAACTGAGTGCAACACCTGACCTT
TCCGGTACGGTGCTGCCACT

Downstream 100 bases:

>100_bases
CCGTGTTGCACTCAGATCCTGCAACCAGCTATTATGATGGACACACCAAATAACTCACTGCATGAGGCAACCCATGAAAA
AGCAATTAAAAATAATTCTG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQMQVRRQVCRPQCKLLPGSER
FELVVHMLREGLSPEQIAGKLRSMNIPSLREAYVCRETIYNAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIP
EMLSIHVRPPEVEDRLMPGHWEGDLIKGKANASAVGTLVERTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTY
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALDAIALQLNMRPRKRFDFKCPI
EVMGEVMQEAVVMRHDAPASIQ

Sequences:

>Translated_342_residues
MSYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQMQVRRQVCRPQCKLLPGSER
FELVVHMLREGLSPEQIAGKLRSMNIPSLREAYVCRETIYNAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIP
EMLSIHVRPPEVEDRLMPGHWEGDLIKGKANASAVGTLVERTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTY
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALDAIALQLNMRPRKRFDFKCPI
EVMGEVMQEAVVMRHDAPASIQ
>Mature_341_residues
SYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQMQVRRQVCRPQCKLLPGSERF
ELVVHMLREGLSPEQIAGKLRSMNIPSLREAYVCRETIYNAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIPE
MLSIHVRPPEVEDRLMPGHWEGDLIKGKANASAVGTLVERTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTYD
QGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALDAIALQLNMRPRKRFDFKCPIE
VMGEVMQEAVVMRHDAPASIQ

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=324, Percent_Identity=41.358024691358, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790736, Length=324, Percent_Identity=41.358024691358, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1787671, Length=324, Percent_Identity=41.358024691358, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38388; Mature: 38257

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30 ; PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQ
CCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
MQVRRQVCRPQCKLLPGSERFELVVHMLREGLSPEQIAGKLRSMNIPSLREAYVCRETIY
HHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
NAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIPEMLSIHVRPPEVEDRLMPGH
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHCCCCCCC
WEGDLIKGKANASAVGTLVERTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTY
CCCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHCCH
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHH
AIALQLNMRPRKRFDFKCPIEVMGEVMQEAVVMRHDAPASIQ
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYHELSIPERATLQLGLAQGFSQRRIARMLGRSPSTISRELRRNRAPGTRYQACAAQQQ
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
MQVRRQVCRPQCKLLPGSERFELVVHMLREGLSPEQIAGKLRSMNIPSLREAYVCRETIY
HHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
NAIYALPVGGLRKELIICLRQGKTSRRPRSGGVDRRGQIPEMLSIHVRPPEVEDRLMPGH
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHCCCCCCC
WEGDLIKGKANASAVGTLVERTSGYLMLIKMADATATSALEGFSAALNGMPLAVRKSMTY
CCCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHCCH
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEALD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHH
AIALQLNMRPRKRFDFKCPIEVMGEVMQEAVVMRHDAPASIQ
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]