Click here to switch to the map view.

The map label for this gene is alkR [H]

Identifier: 117676273

GI number: 117676273

Start: 224184

End: 225119

Strand: Direct

Name: alkR [H]

Synonym: Shewana3_4336

Alternate gene names: 117676273

Gene position: 224184-225119 (Clockwise)

Preceding gene: 117676267

Following gene: 117676274

Centisome position: 80.37

GC content: 44.23

Gene sequence:

>936_bases
ATGGATGTTCTCAGTGAAATTTTAGATAAGGTAGAGCTTACTAGCAGCTACTGGTATAGAACCTCATTTGCGGGAGACTG
GGGCATCAGCCTCCCGCAGGAAGAGAATCTAGCGAGATTTCACATCGTGACTCATGGCGAGTTTTGGTATGAGGTGCCAA
AACTTAAACTTAAAGCATTGGCGGAGCAGGGCGATATTTTGATCTTTTTCAAAGGGATGGAGCACACCATGGCCAGTGCA
CCAAAAATCAAGGCCGAGCCTGCGGTCGAATTTAGAGCTAAAGCCAACCTCACCGAAGCTCAAGTTTTGGAATACGGTGA
TCAGAGCAAGCTCAAAGCCAACGTCGTTTGCGGACATTTTTGTTTTAATGGCGGACCCGATCATCCATTCTTAAATTCGC
TGCCAAATGTCGTGCATATCAAGAGTGCTGATAACTCCCACTCGCCTTGGCTTGCCATGCTTTTAAGTATTATAGAGCAG
GAAGCAAAATCAGGGCTTCCTGGAAGCAACACGTTAGTCAGAAAGCTGACCGAAATTATCTTCGTGCAAGCGTTAAGAAT
CCACATGTACAAATCCAATAAAAATGCTGGATTCTTCAAACTGATCGAAAATCCGCAACTCAGCAAAACCTTGGAAGCTA
TTCATCATAGTCTCGATAAAAAATGGGGTCTTGACGATCTCGCCGGGATTGCGGGGATGTCGCGTACGAACTACAGCGTC
AAATTTAAGGAGCTGTCAGGCATGACACCTCTTGATTATTTAACCTATTGTCGGCTTGAAAAAGCAAAGCAGCTGCTTAA
AGAATCCGGTAAGTCTGTTCCCGAAGTGAGTGAAGCCATTGGGTATCCGGCACATGAACACTTTCAAAAGTTGTTTAAGA
AAAAAATTGGCAAAACTCCTAGCGCGTACAGAAAAGAAAATTCTGAAAAGGTGTAA

Upstream 100 bases:

>100_bases
ACTTACTGATTAGACTGACTTTACAGCATGTAAAATAGATATAATATGTTCAATAGTTCGTATAACATGACCTATTGGAC
AGTTTACGTAGGAGCTATAT

Downstream 100 bases:

>100_bases
AAATGAATATCTATAACTCTCCTGTTACCAAAATAGCTTTCTGGGTCATAGTCATTGGTGGTGCGGCTTGCCTGCTTATC
CCTTTGTTTGCACCTCTTCT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MDVLSEILDKVELTSSYWYRTSFAGDWGISLPQEENLARFHIVTHGEFWYEVPKLKLKALAEQGDILIFFKGMEHTMASA
PKIKAEPAVEFRAKANLTEAQVLEYGDQSKLKANVVCGHFCFNGGPDHPFLNSLPNVVHIKSADNSHSPWLAMLLSIIEQ
EAKSGLPGSNTLVRKLTEIIFVQALRIHMYKSNKNAGFFKLIENPQLSKTLEAIHHSLDKKWGLDDLAGIAGMSRTNYSV
KFKELSGMTPLDYLTYCRLEKAKQLLKESGKSVPEVSEAIGYPAHEHFQKLFKKKIGKTPSAYRKENSEKV

Sequences:

>Translated_311_residues
MDVLSEILDKVELTSSYWYRTSFAGDWGISLPQEENLARFHIVTHGEFWYEVPKLKLKALAEQGDILIFFKGMEHTMASA
PKIKAEPAVEFRAKANLTEAQVLEYGDQSKLKANVVCGHFCFNGGPDHPFLNSLPNVVHIKSADNSHSPWLAMLLSIIEQ
EAKSGLPGSNTLVRKLTEIIFVQALRIHMYKSNKNAGFFKLIENPQLSKTLEAIHHSLDKKWGLDDLAGIAGMSRTNYSV
KFKELSGMTPLDYLTYCRLEKAKQLLKESGKSVPEVSEAIGYPAHEHFQKLFKKKIGKTPSAYRKENSEKV
>Mature_311_residues
MDVLSEILDKVELTSSYWYRTSFAGDWGISLPQEENLARFHIVTHGEFWYEVPKLKLKALAEQGDILIFFKGMEHTMASA
PKIKAEPAVEFRAKANLTEAQVLEYGDQSKLKANVVCGHFCFNGGPDHPFLNSLPNVVHIKSADNSHSPWLAMLLSIIEQ
EAKSGLPGSNTLVRKLTEIIFVQALRIHMYKSNKNAGFFKLIENPQLSKTLEAIHHSLDKKWGLDDLAGIAGMSRTNYSV
KFKELSGMTPLDYLTYCRLEKAKQLLKESGKSVPEVSEAIGYPAHEHFQKLFKKKIGKTPSAYRKENSEKV

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=193, Percent_Identity=29.0155440414508, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI226510985, Length=141, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789993, Length=130, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 35041; Mature: 35041

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLSEILDKVELTSSYWYRTSFAGDWGISLPQEENLARFHIVTHGEFWYEVPKLKLKAL
CCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
AEQGDILIFFKGMEHTMASAPKIKAEPAVEFRAKANLTEAQVLEYGDQSKLKANVVCGHF
HCCCCEEEEEECCHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEEH
CFNGGPDHPFLNSLPNVVHIKSADNSHSPWLAMLLSIIEQEAKSGLPGSNTLVRKLTEII
CCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FVQALRIHMYKSNKNAGFFKLIENPQLSKTLEAIHHSLDKKWGLDDLAGIAGMSRTNYSV
HHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEE
KFKELSGMTPLDYLTYCRLEKAKQLLKESGKSVPEVSEAIGYPAHEHFQKLFKKKIGKTP
EEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
SAYRKENSEKV
HHHHCCCCCCC
>Mature Secondary Structure
MDVLSEILDKVELTSSYWYRTSFAGDWGISLPQEENLARFHIVTHGEFWYEVPKLKLKAL
CCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
AEQGDILIFFKGMEHTMASAPKIKAEPAVEFRAKANLTEAQVLEYGDQSKLKANVVCGHF
HCCCCEEEEEECCHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEEH
CFNGGPDHPFLNSLPNVVHIKSADNSHSPWLAMLLSIIEQEAKSGLPGSNTLVRKLTEII
CCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FVQALRIHMYKSNKNAGFFKLIENPQLSKTLEAIHHSLDKKWGLDDLAGIAGMSRTNYSV
HHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEE
KFKELSGMTPLDYLTYCRLEKAKQLLKESGKSVPEVSEAIGYPAHEHFQKLFKKKIGKTP
EEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
SAYRKENSEKV
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]