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The map label for this gene is insH10 [H]

Identifier: 117676082

GI number: 117676082

Start: 1491

End: 2471

Strand: Reverse

Name: insH10 [H]

Synonym: Shewana3_4142

Alternate gene names: 117676082

Gene position: 2471-1491 (Counterclockwise)

Preceding gene: 117676083

Following gene: NA

Centisome position: 0.89

GC content: 47.6

Gene sequence:

>981_bases
ATGGAACATCAACTCACATTTGCCGATAGCGAATTCTCCAGTAAACGCCGTCAGACCCGTAAGGAAATCTTCCTGTCTCG
GATGGATGCGATTCTTCCTTGGGCAAAACTCCTTGCTGTTATCGAACCTGTTTACCCTAAAGCTGGCAATGGCCGCCGTC
CTTATCCATTGGAAACCATGGTGCGTATCCACTGCATGCAGCACTGGTACAACCTAAGTGATGGTGCCATGGAAGATGCG
TTCTATGAAATTGCATCAATGCGCTTGTTTGCACATCTATCCTTAGATAGCGCCATGCCTGACCGCACGACCATCATGAA
TTTTCGCCATCTGTTGGAGCAGCATAAGCTGGCCCGTAAGATACTCAGCACCATAAACCAATGGCTCAGTGAATGCGGTG
TCATGATGACGCAAGGCACGTTAGTTGATGCAACGATTATCGAAGCGCCGAGCTCAACTAAAAATAAGCAAAACCAACGT
GACGGCGATATGCACCAAACCAAGAAAGGCAACCAATGGCACTTTGGGATGAAGGTACATATCGGCGTCGACGCCAAAAG
TGGCTTAACCCACAGCTTGGTGACCACGTCAGCGAACGAGCACGATTTAAATCAGCTGGAAAACTTGCTACACGGTGAAG
AACAATTTGTGTCAGCCGATGCCGGTTATCAAGGCGCAGAAAAGCGCAAGGTACTTGCAGATAAGAAGGTCGATTGGCTG
ATAGCAGAACGTCCGGGCAAAGTCAGAACCCTCAAGCAACATCCACGTCTCAATAAAGTGCTCATCAGAACTGAGTACTT
GAAGGCTAGCATTAGAGCTAAGGTAGAACATCCATTCCGCATCATTAAGTGCCAATTTGGCTTTGTGAAAGCTCGCTATA
AAGGCTTGGTGAAAAACGATAATCATCTGGCGATGTTATTTGCGTTGGCCAACATCGTCAGAGTGGACCAGATGTTACGC
TATGAGCCGAAGACTACCTAA

Upstream 100 bases:

>100_bases
CTAAACATCACAAGTAGCCAGTTAAACCTAGGGATGATGCGAATAAGTCCCTGATATGAGATCATGTACTCATCCAGTTT
GCCAAGGAACAGGTCAGCTC

Downstream 100 bases:

>100_bases
AATCGGGTAGAAGCCAGAAAACTGGCTAAAAGTGGGATAAAACTGCTGTTTTTTTGCCCAAGACGGGAAAATCGGTGCGG
AAAAAGTAGAGGAAGAAACT

Product: IS4 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MEHQLTFADSEFSSKRRQTRKEIFLSRMDAILPWAKLLAVIEPVYPKAGNGRRPYPLETMVRIHCMQHWYNLSDGAMEDA
FYEIASMRLFAHLSLDSAMPDRTTIMNFRHLLEQHKLARKILSTINQWLSECGVMMTQGTLVDATIIEAPSSTKNKQNQR
DGDMHQTKKGNQWHFGMKVHIGVDAKSGLTHSLVTTSANEHDLNQLENLLHGEEQFVSADAGYQGAEKRKVLADKKVDWL
IAERPGKVRTLKQHPRLNKVLIRTEYLKASIRAKVEHPFRIIKCQFGFVKARYKGLVKNDNHLAMLFALANIVRVDQMLR
YEPKTT

Sequences:

>Translated_326_residues
MEHQLTFADSEFSSKRRQTRKEIFLSRMDAILPWAKLLAVIEPVYPKAGNGRRPYPLETMVRIHCMQHWYNLSDGAMEDA
FYEIASMRLFAHLSLDSAMPDRTTIMNFRHLLEQHKLARKILSTINQWLSECGVMMTQGTLVDATIIEAPSSTKNKQNQR
DGDMHQTKKGNQWHFGMKVHIGVDAKSGLTHSLVTTSANEHDLNQLENLLHGEEQFVSADAGYQGAEKRKVLADKKVDWL
IAERPGKVRTLKQHPRLNKVLIRTEYLKASIRAKVEHPFRIIKCQFGFVKARYKGLVKNDNHLAMLFALANIVRVDQMLR
YEPKTT
>Mature_326_residues
MEHQLTFADSEFSSKRRQTRKEIFLSRMDAILPWAKLLAVIEPVYPKAGNGRRPYPLETMVRIHCMQHWYNLSDGAMEDA
FYEIASMRLFAHLSLDSAMPDRTTIMNFRHLLEQHKLARKILSTINQWLSECGVMMTQGTLVDATIIEAPSSTKNKQNQR
DGDMHQTKKGNQWHFGMKVHIGVDAKSGLTHSLVTTSANEHDLNQLENLLHGEEQFVSADAGYQGAEKRKVLADKKVDWL
IAERPGKVRTLKQHPRLNKVLIRTEYLKASIRAKVEHPFRIIKCQFGFVKARYKGLVKNDNHLAMLFALANIVRVDQMLR
YEPKTT

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1788303, Length=320, Percent_Identity=84.6875, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789921, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1789612, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1789355, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1788518, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1788342, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1787592, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786877, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786764, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786453, Length=320, Percent_Identity=84.375, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1787634, Length=320, Percent_Identity=84.0625, Blast_Score=576, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37507; Mature: 37507

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHQLTFADSEFSSKRRQTRKEIFLSRMDAILPWAKLLAVIEPVYPKAGNGRRPYPLETM
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VRIHCMQHWYNLSDGAMEDAFYEIASMRLFAHLSLDSAMPDRTTIMNFRHLLEQHKLARK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ILSTINQWLSECGVMMTQGTLVDATIIEAPSSTKNKQNQRDGDMHQTKKGNQWHFGMKVH
HHHHHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCEEEEEEEE
IGVDAKSGLTHSLVTTSANEHDLNQLENLLHGEEQFVSADAGYQGAEKRKVLADKKVDWL
EECCCCCCCCHHHEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCCCCCEE
IAERPGKVRTLKQHPRLNKVLIRTEYLKASIRAKVEHPFRIIKCQFGFVKARYKGLVKND
EECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCC
NHLAMLFALANIVRVDQMLRYEPKTT
CCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEHQLTFADSEFSSKRRQTRKEIFLSRMDAILPWAKLLAVIEPVYPKAGNGRRPYPLETM
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VRIHCMQHWYNLSDGAMEDAFYEIASMRLFAHLSLDSAMPDRTTIMNFRHLLEQHKLARK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ILSTINQWLSECGVMMTQGTLVDATIIEAPSSTKNKQNQRDGDMHQTKKGNQWHFGMKVH
HHHHHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCEEEEEEEE
IGVDAKSGLTHSLVTTSANEHDLNQLENLLHGEEQFVSADAGYQGAEKRKVLADKKVDWL
EECCCCCCCCHHHEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCCCCCEE
IAERPGKVRTLKQHPRLNKVLIRTEYLKASIRAKVEHPFRIIKCQFGFVKARYKGLVKND
EECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCC
NHLAMLFALANIVRVDQMLRYEPKTT
CCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]