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The map label for this gene is actII-2 [H]

Identifier: 116874190

GI number: 116874190

Start: 2809196

End: 2810608

Strand: Direct

Name: actII-2 [H]

Synonym: lwe2774

Alternate gene names: 116874190

Gene position: 2809196-2810608 (Clockwise)

Preceding gene: 116874187

Following gene: NA

Centisome position: 99.82

GC content: 38.57

Gene sequence:

>1413_bases
ATGGAACAGACGAATAAACGTAAAACTTTAGCACTTATTTCAATTATGCTTGGTGCTTTTATTTCTTTACTAGATACAAC
TATTGTCAACGTTGCTTTACCAGATATAACGACTGCTTTACACGCTACAAGTGAAACAATCGAATGGGTTATTTCAGGCT
ATGCGCTCGCTTTTGGACTTGTACTTATTTTAGCCGGTCGACTTGGAGATAAATTTGGCCGAAAAAATGTCTATATTATC
GGGATTACGCTCTTTCTAATTATGAGTGTTACAGCTGGCTTTGCTAATTCGGAAAACAGCTTAATTATTTCCCGGGTAAT
ACAAGGCCTCGCTGCTGGGTTATTTTTTCCGCAAATCAATGCCACAATTATGGATATGTATTCGGGGGAAAATCTCGGGA
AAATCTTTGGCATTCTTGGCTCGGTTATTGGAGTTGGAACAGCAATCGGACCTCTTACAGGTGGTCTTTTAATTGAACTT
TTTGGCGTTACAAATGGATGGCGCGCAGTCTTCTTCGTAAATGTTCCTTTTGTTTTAGTAACACTTGTACTGGCTATGCT
TTATCTTCCTAAAAGAACTGTTTCCACGAAAAAAATCAGTTTCGATTTACCTGGAGTTGGGCTACTGACAATCGCACTCT
TACTACTACTTTTCCCACTTATTTCAAATGGTGCAAATGATTTTAGACCAATGGATTATCTTTTAATGGCATTGTCTATT
CCTCTCTTTATTATCCTTTATAAATGGAGCGTCTATCAAGAAAAGAAAGGAAATCAGCCATTAATTGCACCCAACCTACT
TAAAAACAATCAATTTGTTTCAGGTATGCTTTTATCCCTTGTTTACTTTGCTGCTTTTACAAGTATTTTCTTCGTACTAT
CGCTAACTTGGCAAACTGGTTTCAACCGTTCGGCTATTTTATCTGGGCTTGCTATTAGCCCTTTTGCATTAGGTAGTGTG
CTCGCTGCATCTAACAGTTACCGACTTATCCCGTTACTCGGAAGAAAACTTTTAATGCTTGGAGTCTCGCTCGTTATTGT
TGGTCTCGGCACCGTTTCTATTGTTTTCCATCTGAATAATGGCGCTTTTTCTGCTTGGTTCTTATTTTTACCACTTTTCA
TTGCTGGAATTGGTAGTGGTTTGACTATTGCGCCACTGAATAGTTTTACACTTTCCACAGTAACGGGTATAAATCGCGGT
GGTGCAAGCGGGATGTTTAATACAGCCCAACGAATCGGTTCATCCTTTGGAATTGCTGTGGTTGGCTCCGTTTTTTTCCG
TACATTAGGAAATACAACTGCCACTTCAAAAGCGAGTGCCTTTTCAGACAGTTTGCAAATGAGCATGTATGTTAATATTA
TTTTACTCGTTGTTTGTTTCCTTCTCATATTCCGTTTACCAAAAAATATCTAG

Upstream 100 bases:

>100_bases
GTAAAATGTTTCACGTGAAACATTCGCTCTTTCTTTATCATAATAGTATTTAGGTTTATAATGGAAAATATCTCAAAAGC
TTCAAAAAGGAGAATGATTC

Downstream 100 bases:

>100_bases
AAAAAGAGTACGGAGATATATCCTTGATTGGGAATGTATATCTCCGTACTCTTTATATATGTTTCACGTGAAACATTTTA
TTTATAAACAACCTCAAACT

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEQTNKRKTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNVYII
GITLFLIMSVTAGFANSENSLIISRVIQGLAAGLFFPQINATIMDMYSGENLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGVTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFRPMDYLLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPLLGRKLLMLGVSLVIVGLGTVSIVFHLNNGAFSAWFLFLPLFIAGIGSGLTIAPLNSFTLSTVTGINRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTTATSKASAFSDSLQMSMYVNIILLVVCFLLIFRLPKNI

Sequences:

>Translated_470_residues
MEQTNKRKTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNVYII
GITLFLIMSVTAGFANSENSLIISRVIQGLAAGLFFPQINATIMDMYSGENLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGVTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFRPMDYLLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPLLGRKLLMLGVSLVIVGLGTVSIVFHLNNGAFSAWFLFLPLFIAGIGSGLTIAPLNSFTLSTVTGINRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTTATSKASAFSDSLQMSMYVNIILLVVCFLLIFRLPKNI
>Mature_470_residues
MEQTNKRKTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNVYII
GITLFLIMSVTAGFANSENSLIISRVIQGLAAGLFFPQINATIMDMYSGENLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGVTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFRPMDYLLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPLLGRKLLMLGVSLVIVGLGTVSIVFHLNNGAFSAWFLFLPLFIAGIGSGLTIAPLNSFTLSTVTGINRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTTATSKASAFSDSLQMSMYVNIILLVVCFLLIFRLPKNI

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=431, Percent_Identity=27.1461716937355, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=29.2270531400966, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788710, Length=444, Percent_Identity=23.4234234234234, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789042, Length=450, Percent_Identity=24.8888888888889, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788392, Length=458, Percent_Identity=23.7991266375546, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790146, Length=174, Percent_Identity=26.4367816091954, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI48994889, Length=171, Percent_Identity=28.0701754385965, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=175, Percent_Identity=29.7142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=300, Percent_Identity=22.6666666666667, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50433; Mature: 50433

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTNKRKTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLILAGRLGDKFGRKNVYIIGITLFLIMSVTAGFANSENSLIISRVIQGLAAGLFFPQIN
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
ATIMDMYSGENLGKIFGILGSVIGVGTAIGPLTGGLLIELFGVTNGWRAVFFVNVPFVLV
HHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHH
TLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFRPMDYLLMALSI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTG
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNRSAILSGLAISPFALGSVLAASNSYRLIPLLGRKLLMLGVSLVIVGLGTVSIVFHLNN
CCHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
GAFSAWFLFLPLFIAGIGSGLTIAPLNSFTLSTVTGINRGGASGMFNTAQRIGSSFGIAV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHH
VGSVFFRTLGNTTATSKASAFSDSLQMSMYVNIILLVVCFLLIFRLPKNI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQTNKRKTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLILAGRLGDKFGRKNVYIIGITLFLIMSVTAGFANSENSLIISRVIQGLAAGLFFPQIN
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
ATIMDMYSGENLGKIFGILGSVIGVGTAIGPLTGGLLIELFGVTNGWRAVFFVNVPFVLV
HHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHH
TLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFRPMDYLLMALSI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTG
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNRSAILSGLAISPFALGSVLAASNSYRLIPLLGRKLLMLGVSLVIVGLGTVSIVFHLNN
CCHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCEEEEEEEECC
GAFSAWFLFLPLFIAGIGSGLTIAPLNSFTLSTVTGINRGGASGMFNTAQRIGSSFGIAV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHH
VGSVFFRTLGNTTATSKASAFSDSLQMSMYVNIILLVVCFLLIFRLPKNI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]