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The map label for this gene is acsA [H]

Identifier: 116874088

GI number: 116874088

Start: 2703259

End: 2704836

Strand: Direct

Name: acsA [H]

Synonym: lwe2672

Alternate gene names: 116874088

Gene position: 2703259-2704836 (Clockwise)

Preceding gene: 116874078

Following gene: 116874093

Centisome position: 96.06

GC content: 40.49

Gene sequence:

>1578_bases
ATGAATTCAGAGAACCTAATTGCTCCAGAACTTTATAATATCACCGACGAGATTGCTAAATTTAGTTCTGAAAAAACTGC
CTTAATTTGGAAGAATGACCACGATGAAACAAAAACTTGGAGTTATCGTCACCTGCTTGAACAAGCGAATAAATTTGCTA
ATGTGGTAAAAAATGCTGGTGTTAAAAAAGGAGATCACGTCATTGTTATGACACCTAGAGCACTTGAAACTTACGCCATC
TATATGGGTTTGTGGAAAGCTGGGGCTATTATCATTCCCGCATCCGAACTACTTAAAGCCCATGATTTAGAATACCGTAT
TCACCACGCAAACGTAAAAGCAATCGTTTCTTACAGCGGAATGACAGCAGAATTTGATAAAATTGAAAAAATTCCTTCTG
TATCGAAAAAAATTATTGTTGGTGGCAAATTATCCGGCTGGGATCATTATGAAACATTAATGGATTCGGCTTCTACTGAT
TTTGAACGCGTAGAAACTTCTCGCGATGATGCTTGCCTGCTTGCTTTCACAAGTGGCACAACTGGTAATCCTAAAGGGGT
TGTACATATTCATGGTTGGGGTTATGCACATATTCGTATTGCAGCTGATCATTGGCTCGATATTCATGAAGATGATATCG
TCTGGGCTACAGCAGGACCTGGTTGGCAAAAATGGGTTTGGTCTCCTTTCCTTTCTGTACTTGGAAAAGGCGCGACCGGT
TTTATTTACAGCGGTCGTTTCATTCCTGAAAAACAACTTCATTTACTAGAAGAAGAAAAAATTAACGTACTATGTTGTAC
GCCAACGGAATATCGCTTAATGGCAAAAGTAAATCATTTACGTGAACATGATTTAAGTTCGCTTCGTAGCGCCGTTTCAG
CAGGAGAACCTTTAAACCGAGAAGTTATTCAAGTTTTCCAAGATAATTTTAACATTAAAGTCCGTGATGGCTACGGTCAG
ACGGAAAGTACCCTTTTGATTGGAACACTTGTTGATACACCAATTCGTCCTGGGTCAATGGGTAAACCAATTATGCCTGA
ATACATGGCGATTATTGATGCAGATGGAAATCCTGTCGGGGTTGGCGAAATTGGTGATATCGCTATGCGTAAAGACTTCC
CGGCACTTTTCAAAGAGTATTATAAGGAACCCGAACGCTTGCAAAAAGCGATTCGTGGTGATTATTTTGTTTCTGGTGAC
CGTGCAATCCGTGATGAAGATAATTATTACTGGTTCCAAGGCCGGAATGATGACATTATTATTAGCTCCGGATACACCAT
CGGACCATTTGAAGTTGAGGACGCGCTCACACATCACCCCGCTGTAAAAGAAGTCGCTGTTGTCGCAAGTCCTGACGAAA
TCCGTGGTACTGTTGTAAAAGCGTTCATCGTTTTAAAAGATGGATACGAGGGCACCGAGGATCTTGTTCATGAATTACAA
ACATTTACAAAAGAACAAACTGCTCCATATAAATACCCTCGCCGAATTGAATTTGTTGAGTCATTACCGAAAACTGATTC
TGGTAAAATTCGCCGCGTCGAGTTGCGTGATGCGGAGTTTGCTAGTGTTAATAAATAA

Upstream 100 bases:

>100_bases
TTTAGTTGCTTTCAAGTAACATTTATATTACCATATATTAATACTAGGTACTAAAACAAAATTATCAAAATATACAATCT
ACTTATAAAGGAGAATCATT

Downstream 100 bases:

>100_bases
AGAAATAGAAGTTCTCATTGGAGGACTTCTATTTCTTTATTTATTCGCTATACCACTGTTTTTTGGTGAAGCCATGCTCT
TTTCGAAACGATTCTGTATA

Product: acetyl-coenzyme A synthetase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNDHDETKTWSYRHLLEQANKFANVVKNAGVKKGDHVIVMTPRALETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYSGMTAEFDKIEKIPSVSKKIIVGGKLSGWDHYETLMDSASTD
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYSGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNHLREHDLSSLRSAVSAGEPLNREVIQVFQDNFNIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDADGNPVGVGEIGDIAMRKDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVESLPKTDSGKIRRVELRDAEFASVNK

Sequences:

>Translated_525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNDHDETKTWSYRHLLEQANKFANVVKNAGVKKGDHVIVMTPRALETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYSGMTAEFDKIEKIPSVSKKIIVGGKLSGWDHYETLMDSASTD
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYSGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNHLREHDLSSLRSAVSAGEPLNREVIQVFQDNFNIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDADGNPVGVGEIGDIAMRKDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVESLPKTDSGKIRRVELRDAEFASVNK
>Mature_525_residues
MNSENLIAPELYNITDEIAKFSSEKTALIWKNDHDETKTWSYRHLLEQANKFANVVKNAGVKKGDHVIVMTPRALETYAI
YMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYSGMTAEFDKIEKIPSVSKKIIVGGKLSGWDHYETLMDSASTD
FERVETSRDDACLLAFTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
FIYSGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNHLREHDLSSLRSAVSAGEPLNREVIQVFQDNFNIKVRDGYGQ
TESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDADGNPVGVGEIGDIAMRKDFPALFKEYYKEPERLQKAIRGDYFVSGD
RAIRDEDNYYWFQGRNDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
TFTKEQTAPYKYPRRIEFVESLPKTDSGKIRRVELRDAEFASVNK

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=534, Percent_Identity=36.5168539325843, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI122937307, Length=539, Percent_Identity=37.6623376623377, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI38505220, Length=537, Percent_Identity=36.8715083798883, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI58082049, Length=512, Percent_Identity=37.5, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=37.109375, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=37.109375, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI115511026, Length=536, Percent_Identity=34.7014925373134, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI28416953, Length=553, Percent_Identity=29.8372513562387, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI8923896, Length=570, Percent_Identity=28.4210526315789, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI253970489, Length=581, Percent_Identity=28.2271944922547, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI42544134, Length=348, Percent_Identity=30.7471264367816, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI195947378, Length=407, Percent_Identity=27.7641277641278, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI13375727, Length=558, Percent_Identity=25.8064516129032, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI156151445, Length=545, Percent_Identity=26.7889908256881, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI187761345, Length=469, Percent_Identity=27.0788912579957, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI187761343, Length=469, Percent_Identity=27.0788912579957, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=29.9256505576208, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788107, Length=512, Percent_Identity=26.5625, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=25.8126195028681, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1786529, Length=554, Percent_Identity=27.4368231046931, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI145693145, Length=495, Percent_Identity=28.0808080808081, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786810, Length=501, Percent_Identity=25.3493013972056, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789201, Length=381, Percent_Identity=25.9842519685039, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=555, Percent_Identity=28.6486486486486, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71982997, Length=555, Percent_Identity=28.6486486486486, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17558820, Length=496, Percent_Identity=25.8064516129032, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=26.6019417475728, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=526, Percent_Identity=26.9961977186312, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=25.8188824662813, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=526, Percent_Identity=24.3346007604563, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=522, Percent_Identity=25.095785440613, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=522, Percent_Identity=25.095785440613, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=522, Percent_Identity=25.095785440613, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=25.0980392156863, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=22.4448897795591, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=522, Percent_Identity=23.5632183908046, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=23.3521657250471, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=389, Percent_Identity=26.2210796915167, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=558, Percent_Identity=30.1075268817204, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=27.6481149012567, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319699, Length=520, Percent_Identity=24.8076923076923, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24667955, Length=555, Percent_Identity=27.5675675675676, Blast_Score=188, Evalue=9e-48,
Organism=Drosophila melanogaster, GI62472339, Length=528, Percent_Identity=27.8409090909091, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=27.1317829457364, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24667959, Length=362, Percent_Identity=30.3867403314917, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355181, Length=497, Percent_Identity=26.3581488933602, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21355225, Length=523, Percent_Identity=23.9005736137667, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18859661, Length=508, Percent_Identity=23.2283464566929, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=23.4817813765182, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24656500, Length=519, Percent_Identity=22.5433526011561, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648257, Length=504, Percent_Identity=23.6111111111111, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=22.0440881763527, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648260, Length=391, Percent_Identity=24.5524296675192, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653035, Length=411, Percent_Identity=23.8442822384428, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648676, Length=571, Percent_Identity=22.5919439579685, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161076582, Length=419, Percent_Identity=22.673031026253, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=507, Percent_Identity=24.4575936883629, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356441, Length=356, Percent_Identity=24.438202247191, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=485, Percent_Identity=22.8865979381443, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=22.8865979381443, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 59153; Mature: 59153

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSENLIAPELYNITDEIAKFSSEKTALIWKNDHDETKTWSYRHLLEQANKFANVVKNAG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VKKGDHVIVMTPRALETYAIYMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYSG
CCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCEEEEEECCEEEEEEECC
MTAEFDKIEKIPSVSKKIIVGGKLSGWDHYETLMDSASTDFERVETSRDDACLLAFTSGT
CCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCC
TGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
CCCCCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCE
FIYSGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNHLREHDLSSLRSAVSAGEPLNR
EEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVIQVFQDNFNIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDADGNPVG
HHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEC
VGEIGDIAMRKDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGRNDDII
CCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEECCCCEEECCCCEEEEECCCCCEE
ISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
EECCCEECCEEHHHHHHCCCCHHHEEEEECCHHHHHHEEEEEEEEECCCCCHHHHHHHHH
TFTKEQTAPYKYPRRIEFVESLPKTDSGKIRRVELRDAEFASVNK
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNSENLIAPELYNITDEIAKFSSEKTALIWKNDHDETKTWSYRHLLEQANKFANVVKNAG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VKKGDHVIVMTPRALETYAIYMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYSG
CCCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCEEEEEECCEEEEEEECC
MTAEFDKIEKIPSVSKKIIVGGKLSGWDHYETLMDSASTDFERVETSRDDACLLAFTSGT
CCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEEEEEECCC
TGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLGKGATG
CCCCCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCE
FIYSGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNHLREHDLSSLRSAVSAGEPLNR
EEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EVIQVFQDNFNIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDADGNPVG
HHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEC
VGEIGDIAMRKDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGRNDDII
CCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEECCCCEEECCCCEEEEECCCCCEE
ISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDLVHELQ
EECCCEECCEEHHHHHHCCCCHHHEEEEECCHHHHHHEEEEEEEEECCCCCHHHHHHHHH
TFTKEQTAPYKYPRRIEFVESLPKTDSGKIRRVELRDAEFASVNK
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]