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The map label for this gene is dak1 [H]

Identifier: 116874060

GI number: 116874060

Start: 2677310

End: 2678299

Strand: Direct

Name: dak1 [H]

Synonym: lwe2644

Alternate gene names: 116874060

Gene position: 2677310-2678299 (Clockwise)

Preceding gene: 116874055

Following gene: 116874061

Centisome position: 95.14

GC content: 42.32

Gene sequence:

>990_bases
ATGAAGAAGATTCTTAACGGTACAGATCAAGTAGTAGAACAAATGGTGGAAGGTTTAGTTAAATCGCATGCAGATATTGT
TCACCGTGTGGAAGGAACTCGCGTCATTGCAAGAAATGATAAACGCCCAAAAAAAGTCGGACTTGTTAGTGGCGGAGGTT
CTGGTCACGAGCCTGCCCATGCTGGTTATGTAGGTCGTGGAATGTTATCTGCGGCAGTGTGCGGCGATGTTTTCACTTCT
CCAACTCCAGACCAAATTTATGAAGGTATTAAAGCCGCTGACCAAGGTGCCGGTGTGCTTTTAATCGTAAAAAACTATAC
CGGTGATGTGATGAACTTTGAGATGGCAGCAGATTTAGCAGATGCCGAGGATATTAAAGTAGAACAAATCGTAGTAGATG
ATGATATTGCTGTTGAAGATAGTACTTTTACAACTGGGCGTCGCGGTGTTGCTGGAACGGTTCTAGTGCATAAAATAATC
GGTGCAGCTGCAGAGGCTGGTGCATCGCTTGAAGAACTGAAAGCACTTGGTGAAAAAGTAATTGCCTCTGTTAAAACGCT
AGGTGTAGCACTTTCTCCTTGTACAGTTCCTGAGGTTGGACATCCTGGATTTGAGCTCGCTGATGACGAAATTGAGCTTG
GTATCGGTATCCACGGTGAACCTGGATTTACAAAAGAAAAAATCATGCCATCCGCAAGTCTTGCTAAACAACTTTTTGAC
CGCATTAGTAAAGAAAGTAAACTCCTACCTGGAGATAAAGTAGTTGTCCTAGTAAATGGCATGGGGGCAACACCACTAAT
GGAACAATATGTTTTTGCAAATGATGTCCATGAACTTCTAAAAAATGCTGGAATAAGCGTAGAAAAAACACTTGTTGGAG
ATTATATGACTTCCCTAGAAATGGCCGGATTGTCACTCACCATTTTGAAATTAGAAGATGAGAAATGGGTCGATATGTTG
AAACTCCCAGTGGACACAATTGCTTGGTAA

Upstream 100 bases:

>100_bases
CATTTTATAAGTTGTATAATAAAATAAGCAACCACGTTTTGACTTTCTAGTAAAGGGAAATTGAGATAAGAGGACTATTT
TGAAGGAGGTTTTTTGTCAG

Downstream 100 bases:

>100_bases
CAAAAATTTGGAGGAATGAAAAATGACTTATAATAAAGATTGGGCGTTACGTTGGTTAAATGATTTCGGCGAACGCGTAC
AAGAAAACAAACAATTATTA

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKILNGTDQVVEQMVEGLVKSHADIVHRVEGTRVIARNDKRPKKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADAEDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLEELKALGEKVIASVKTLGVALSPCTVPEVGHPGFELADDEIELGIGIHGEPGFTKEKIMPSASLAKQLFD
RISKESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGISVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW

Sequences:

>Translated_329_residues
MKKILNGTDQVVEQMVEGLVKSHADIVHRVEGTRVIARNDKRPKKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADAEDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLEELKALGEKVIASVKTLGVALSPCTVPEVGHPGFELADDEIELGIGIHGEPGFTKEKIMPSASLAKQLFD
RISKESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGISVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW
>Mature_329_residues
MKKILNGTDQVVEQMVEGLVKSHADIVHRVEGTRVIARNDKRPKKVGLVSGGGSGHEPAHAGYVGRGMLSAAVCGDVFTS
PTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLADAEDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKII
GAAAEAGASLEELKALGEKVIASVKTLGVALSPCTVPEVGHPGFELADDEIELGIGIHGEPGFTKEKIMPSASLAKQLFD
RISKESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGISVEKTLVGDYMTSLEMAGLSLTILKLEDEKWVDML
KLPVDTIAW

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=341, Percent_Identity=41.3489736070381, Blast_Score=241, Evalue=7e-64,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=43.1818181818182, Blast_Score=295, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17565018, Length=329, Percent_Identity=37.0820668693009, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321055, Length=338, Percent_Identity=38.4615384615385, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6323570, Length=281, Percent_Identity=40.2135231316726, Blast_Score=175, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34926; Mature: 34926

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILNGTDQVVEQMVEGLVKSHADIVHRVEGTRVIARNDKRPKKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCC
AGYVGRGMLSAAVCGDVFTSPTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHCCC
DAEDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKIIGAAAEAGASLEELKALGEKV
CCCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASVKTLGVALSPCTVPEVGHPGFELADDEIELGIGIHGEPGFTKEKIMPSASLAKQLFD
HHHHHHHHEEECCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHH
RISKESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGISVEKTLVGDYMTSLE
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
MAGLSLTILKLEDEKWVDMLKLPVDTIAW
HCCCEEEEEEECCHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MKKILNGTDQVVEQMVEGLVKSHADIVHRVEGTRVIARNDKRPKKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCC
AGYVGRGMLSAAVCGDVFTSPTPDQIYEGIKAADQGAGVLLIVKNYTGDVMNFEMAADLA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHCCC
DAEDIKVEQIVVDDDIAVEDSTFTTGRRGVAGTVLVHKIIGAAAEAGASLEELKALGEKV
CCCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IASVKTLGVALSPCTVPEVGHPGFELADDEIELGIGIHGEPGFTKEKIMPSASLAKQLFD
HHHHHHHHEEECCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHH
RISKESKLLPGDKVVVLVNGMGATPLMEQYVFANDVHELLKNAGISVEKTLVGDYMTSLE
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
MAGLSLTILKLEDEKWVDMLKLPVDTIAW
HCCCEEEEEEECCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]