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The map label for this gene is mltD [C]

Identifier: 116874055

GI number: 116874055

Start: 2670872

End: 2672635

Strand: Direct

Name: mltD [C]

Synonym: lwe2639

Alternate gene names: 116874055

Gene position: 2670872-2672635 (Clockwise)

Preceding gene: 116874050

Following gene: 116874060

Centisome position: 94.91

GC content: 37.41

Gene sequence:

>1764_bases
ATGCAAAAGACAAGAAAAGAACGTATTCTTGAGGCTTTACAAGAAGAGAAGAAAAACAAAAAAAGCAAAAAAATAAAAAC
TGGTGCAACAATTGCTGGGGTTACTGCCATAGCAACTTCAATCACTGTTCCTGGAATCGAAGTTATCGTTCAAGCAGATG
AAACTGCACCCGCTGATGAAGCTGTCAAAAGCGCAGATAAAACAGCTCAACCAGAAGCAGATTCTGCTACCAAAACCACT
ACAGAAACGACTCCAGAAAGAAATCAAATAGAACCTAAAACAGAAACTCAACCTAAAACACCTACAGAAAAAACGCCTCC
TGCCGAAAAAGAAGTTACTCCTACAGTGCAAACAGCCACAATAAGCAACTCTGACAATGTAACAAGTTCATCTACACCAG
CAACCTATAATGTGTTACAAAAGTCCGCATTACGCTCAGGCGCTACAGTCCAAAGTTTCATTCAAACAATCCAGTCATCT
TCATCACAAATCGCTGCAAACAATGATTTATACGCCTCCGTAATGATTGCCCAAGCCATTTTAGAAAGTGCTTATGGAAC
AAGCGAATTAGGCTCTGCTCCTAACTACAATTTATTTGGCATTAAAGGCGCATACAACGGTAAGTCTTATACAAAACAAA
CACTAGAAGATGATGGCAAAGGAAACTACTACACAATTACAGCCAAATTCAGAAAATATCCATCTTACCATCAATCTTTA
GAAGATTACGCACAAGTCATTCGAAAAGGCCCAAGCTGGAATTCCAATTACTATTCTAAAGTATGGAAAAGTAATACTAA
ATCGTATAAAGATGCCACTCAAGCATTAACCGGAACTTATGCAACTGACACAGCCTATGCAAGTAAATTAAACAATTTAA
TTAGCACTTATAACCTAACCCAATATGACACTGGAAACGCGTCAAATGGTAGCGGCTCTTCAAATACTGGTAACTCTGGT
AACTCCAACAGTTCCAGTTCAAACAGCGCAACTTACACGGTCGTAAAAGGTGATTCTCTTTGGAAAATTGCCACTAAATA
TAATGTATCAGTAGCCAACTTAAAATCTTGGAATAACCTAAAATCTGACAACATTTACATCGGTCAAAAACTTAAAGTCA
CCGCTGGATCCACGTCAAATACCTCTAAGCCAAACACCGGATCAAATACCAACACATCTAAACCAAGCACTAGCACCAAC
GCAAAAACCTACACTGTAAAAAAAGGCGACTCTCTTTGGAAAATTGCCACTAATAATAAAGTAACTATCGCTAATTTAAA
ATCTTGGAACAATCTAAAGTCTGATTTTATTTATCCAGGGCAAACCCTTAAAGTCAGTGCTAGTTCCACTTCAAGCAACA
CAAACACATCTAAACCAAGTACCGGTTCAAGCACTAACACATCTAAACCAAACACAAACGCAAAAACGTATACCGTGAAA
AAAGGCGACTCCCTATGGAAAATTGCTAATCAAAATAAGACAACTGTCACAAACTTAAAATCTTGGAACAACCTAAAATC
CGACAACATTTACATCGGACAAAAACTTAAAGTCAGCGCTGGATCTATTACAAATAATACCAACACATCTAAACCAAGCA
CTAGCAAACCAAGCAATTCATCCACCAAAACCTACACCGTAAAAAAAGGAGATTCACTTTGGTCCATCTCTAGACAATAC
AAAACAACTGTAGATAATATTAAGTCTTGGAACAAGCTAACAAGTAACACCATTTATATCGGACAAAAAATCACGATTAA
ATAA

Upstream 100 bases:

>100_bases
GACAATTTAATTACAATTTGTACAACCATTACATACCTTCCGTGAAGTGTGTAATAATAGAGGTAGAATATTTATTTAAA
AGAATTGGAAGTGATTTACC

Downstream 100 bases:

>100_bases
GAAAAACGCCTTCTTTACGAGTATATAAAGAAGGCGTTTTATTTAACTAAAAAATACTATTTTCACTCTTTTTCCAAGAC
ATCTTCCAACATTTGTCTCG

Product: autolysin

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Beta-glycosidase; Peptidoglycan hydrolase [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MQKTRKERILEALQEEKKNKKSKKIKTGATIAGVTAIATSITVPGIEVIVQADETAPADEAVKSADKTAQPEADSATKTT
TETTPERNQIEPKTETQPKTPTEKTPPAEKEVTPTVQTATISNSDNVTSSSTPATYNVLQKSALRSGATVQSFIQTIQSS
SSQIAANNDLYASVMIAQAILESAYGTSELGSAPNYNLFGIKGAYNGKSYTKQTLEDDGKGNYYTITAKFRKYPSYHQSL
EDYAQVIRKGPSWNSNYYSKVWKSNTKSYKDATQALTGTYATDTAYASKLNNLISTYNLTQYDTGNASNGSGSSNTGNSG
NSNSSSSNSATYTVVKGDSLWKIATKYNVSVANLKSWNNLKSDNIYIGQKLKVTAGSTSNTSKPNTGSNTNTSKPSTSTN
AKTYTVKKGDSLWKIATNNKVTIANLKSWNNLKSDFIYPGQTLKVSASSTSSNTNTSKPSTGSSTNTSKPNTNAKTYTVK
KGDSLWKIANQNKTTVTNLKSWNNLKSDNIYIGQKLKVSAGSITNNTNTSKPSTSKPSNSSTKTYTVKKGDSLWSISRQY
KTTVDNIKSWNKLTSNTIYIGQKITIK

Sequences:

>Translated_587_residues
MQKTRKERILEALQEEKKNKKSKKIKTGATIAGVTAIATSITVPGIEVIVQADETAPADEAVKSADKTAQPEADSATKTT
TETTPERNQIEPKTETQPKTPTEKTPPAEKEVTPTVQTATISNSDNVTSSSTPATYNVLQKSALRSGATVQSFIQTIQSS
SSQIAANNDLYASVMIAQAILESAYGTSELGSAPNYNLFGIKGAYNGKSYTKQTLEDDGKGNYYTITAKFRKYPSYHQSL
EDYAQVIRKGPSWNSNYYSKVWKSNTKSYKDATQALTGTYATDTAYASKLNNLISTYNLTQYDTGNASNGSGSSNTGNSG
NSNSSSSNSATYTVVKGDSLWKIATKYNVSVANLKSWNNLKSDNIYIGQKLKVTAGSTSNTSKPNTGSNTNTSKPSTSTN
AKTYTVKKGDSLWKIATNNKVTIANLKSWNNLKSDFIYPGQTLKVSASSTSSNTNTSKPSTGSSTNTSKPNTNAKTYTVK
KGDSLWKIANQNKTTVTNLKSWNNLKSDNIYIGQKLKVSAGSITNNTNTSKPSTSKPSNSSTKTYTVKKGDSLWSISRQY
KTTVDNIKSWNKLTSNTIYIGQKITIK
>Mature_587_residues
MQKTRKERILEALQEEKKNKKSKKIKTGATIAGVTAIATSITVPGIEVIVQADETAPADEAVKSADKTAQPEADSATKTT
TETTPERNQIEPKTETQPKTPTEKTPPAEKEVTPTVQTATISNSDNVTSSSTPATYNVLQKSALRSGATVQSFIQTIQSS
SSQIAANNDLYASVMIAQAILESAYGTSELGSAPNYNLFGIKGAYNGKSYTKQTLEDDGKGNYYTITAKFRKYPSYHQSL
EDYAQVIRKGPSWNSNYYSKVWKSNTKSYKDATQALTGTYATDTAYASKLNNLISTYNLTQYDTGNASNGSGSSNTGNSG
NSNSSSSNSATYTVVKGDSLWKIATKYNVSVANLKSWNNLKSDNIYIGQKLKVTAGSTSNTSKPNTGSNTNTSKPSTSTN
AKTYTVKKGDSLWKIATNNKVTIANLKSWNNLKSDFIYPGQTLKVSASSTSSNTNTSKPSTGSSTNTSKPNTNAKTYTVK
KGDSLWKIANQNKTTVTNLKSWNNLKSDNIYIGQKLKVSAGSITNNTNTSKPSTSKPSNSSTKTYTVKKGDSLWSISRQY
KTTVDNIKSWNKLTSNTIYIGQKITIK

Specific function: Hydrolyzes the cell wall of E.faecalis and M.lysodeikticus. May play an important role in cell wall growth and cell separation [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=114, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787321, Length=125, Percent_Identity=33.6, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559362, Length=303, Percent_Identity=23.7623762376238, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013338
- InterPro:   IPR002901
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF01476 LysM [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 63440; Mature: 63440

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTRKERILEALQEEKKNKKSKKIKTGATIAGVTAIATSITVPGIEVIVQADETAPADE
CCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
AVKSADKTAQPEADSATKTTTETTPERNQIEPKTETQPKTPTEKTPPAEKEVTPTVQTAT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ISNSDNVTSSSTPATYNVLQKSALRSGATVQSFIQTIQSSSSQIAANNDLYASVMIAQAI
ECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
LESAYGTSELGSAPNYNLFGIKGAYNGKSYTKQTLEDDGKGNYYTITAKFRKYPSYHQSL
HHHHCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEHHHCCCHHHHH
EDYAQVIRKGPSWNSNYYSKVWKSNTKSYKDATQALTGTYATDTAYASKLNNLISTYNLT
HHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
QYDTGNASNGSGSSNTGNSGNSNSSSSNSATYTVVKGDSLWKIATKYNVSVANLKSWNNL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECEEEECCCCCCCC
KSDNIYIGQKLKVTAGSTSNTSKPNTGSNTNTSKPSTSTNAKTYTVKKGDSLWKIATNNK
CCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCE
VTIANLKSWNNLKSDFIYPGQTLKVSASSTSSNTNTSKPSTGSSTNTSKPNTNAKTYTVK
EEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
KGDSLWKIANQNKTTVTNLKSWNNLKSDNIYIGQKLKVSAGSITNNTNTSKPSTSKPSNS
CCCCEEEECCCCCCEEEECHHCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCC
STKTYTVKKGDSLWSISRQYKTTVDNIKSWNKLTSNTIYIGQKITIK
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEC
>Mature Secondary Structure
MQKTRKERILEALQEEKKNKKSKKIKTGATIAGVTAIATSITVPGIEVIVQADETAPADE
CCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHH
AVKSADKTAQPEADSATKTTTETTPERNQIEPKTETQPKTPTEKTPPAEKEVTPTVQTAT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ISNSDNVTSSSTPATYNVLQKSALRSGATVQSFIQTIQSSSSQIAANNDLYASVMIAQAI
ECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
LESAYGTSELGSAPNYNLFGIKGAYNGKSYTKQTLEDDGKGNYYTITAKFRKYPSYHQSL
HHHHCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEHHHCCCHHHHH
EDYAQVIRKGPSWNSNYYSKVWKSNTKSYKDATQALTGTYATDTAYASKLNNLISTYNLT
HHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCE
QYDTGNASNGSGSSNTGNSGNSNSSSSNSATYTVVKGDSLWKIATKYNVSVANLKSWNNL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECEEEECCCCCCCC
KSDNIYIGQKLKVTAGSTSNTSKPNTGSNTNTSKPSTSTNAKTYTVKKGDSLWKIATNNK
CCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCE
VTIANLKSWNNLKSDFIYPGQTLKVSASSTSSNTNTSKPSTGSSTNTSKPNTNAKTYTVK
EEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
KGDSLWKIANQNKTTVTNLKSWNNLKSDNIYIGQKLKVSAGSITNNTNTSKPSTSKPSNS
CCCCEEEECCCCCCEEEECHHCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCCC
STKTYTVKKGDSLWSISRQYKTTVDNIKSWNKLTSNTIYIGQKITIK
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1679432; 12663927 [H]