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The map label for this gene is ami [H]

Identifier: 116873924

GI number: 116873924

Start: 2547203

End: 2549017

Strand: Direct

Name: ami [H]

Synonym: lwe2508

Alternate gene names: 116873924

Gene position: 2547203-2549017 (Clockwise)

Preceding gene: 116873901

Following gene: 116873930

Centisome position: 90.51

GC content: 38.95

Gene sequence:

>1815_bases
TTGAAAAAATTAGTAAAATCGGCGGTTGTTTTTGCAAGCCTTGCTTTTATTGGCGCCTCCGCTACCATGATTACAGAAAA
TGCAAGTGCTGCTTCCATTGATACAGTACAAAAAGTAGACGATCAATCTATTTATATCCCAAAAGGTATTAGAGACGGGA
CTGCGACGGAAGAACACGATGGCTTTGAAGATGAAACTAATAGCGTACTGAAGGAAGTACCTTTACTACGAGCAGCAGGA
TATCCTGACGTCAATTCTTATATTAAAACAAACAAATTTTCTACCGCTTCCATTGAAAAACAATTAAAAAGTCAATTTCC
CAAGTTTAACTATCGGAATGGTTACGGCAAACCAGAGGGAATTGTTATCCATGAAACAGCTAATAATTCTTCCACTATTA
CAGGTGAAATTAACTATATGAGTACGAACTACAATAACGCTTTTGTTCATGCATTTGTAGATAAATCTCGTATTATTCAA
ATTCATCCTACTGAAAATGGTGTCTGGGGAGCCGGTCAATATGCCAATGCTCGTTTTATCCAAGTCGAACTAGTGCGCTC
CAAAACTTTTGATGAATTTTCCCGTTCTATAAATAACTATGCTTATTATACCGCGTATCTTTTAGATCAATACAAGCTCC
CAGTAGACAGCGCACATAGTGATGGTAAAGGGACTGTCTGGTCACATGATGCCGTAACTCGTTATTTAGGTGGAACCACT
CACACCGATCCCGTAGGCTATTTCAACCAATGGGGTTATAATTTTAATAGTTTTGTTACTTTAGTTAATGAAAAGTATAA
GGCAATTCAATCTAGTAAAGTGACTTATGATAAAATCGAATATGATAAAGGCGTTACCGCTTATGCACGAGTAAAAACTG
CTCCCGGAAATGCTGTTTGGACGAAACCATACAAAACAGAAGGCGCTAAAGGCATTAATCCACTTTCTGTATATGTCGGC
AAAAATATGCGTATCTTACGTGAAGCTAAAACACCAATAACTACTTGGTATCAGTTTAGCATTGATGGTAAAACAATCGG
ATGGGTCGATACTCGCGCTCTGGATACATTTTATAAGCAGAGCATGGAAAAACCGGCCAATTTAACACGTTATGTACTTG
CCAACAAAGCCAACGAAGCTTACTATAAAGTTCCTGTCGCGGATTCAGCGGTCAAATGGGGCACTTTATCCACTTACAAA
AATCAAAAGTTAACAGTTGATAGACAAGCTACTATTGAAGGACAACTATGGTACCGAATAAAAACAAGTTCTACCTTCAT
CGGATGGACGAAAGCAGCCAATTTAACAACTGTTTCTCCAGTCGATAAAATCGAATACGATAAAGGCGTTACCGCTTATG
CACGAGTAAAAACTGCTCCCGGAAATGCTGTTTGGACGAAACCATACAAAACAGAAGGAGCTAAAGGCGTTAACCCACTT
TCTGTATATGTCGGCAAAAATATGCGTATCTTACGCGAAGCCAAAACACCAATAACCACTTGGTATCAGTTTAGCATTGA
TGGTAAAACAATCGGATGGGTCGATACCCGCGCTCTGAATACGTTTTATAAGCAGAGCATGGAAAAACCGGCCAATTTAA
CACGTTATGTACTTGCCAACAAAGCCAACGAAGCTTACTATAAAGTTCCTGTCGCGGATTCAGCGGTTAAATGGGGCACT
TTATCCACTTACAAAAATCAAAAGTTAACAGTTGATAGACAAGCTACTATTGAAGGACAACTATGGTACCGAATAAAAAC
AAGTTCTACCTTTATCGGGTGGACGAAAGCGGCAAACCTTAGTACTAGCAAATAA

Upstream 100 bases:

>100_bases
GCCAATTCCCATTGACTACAACCTAACGTGTTGTCATAATTAGTATAAGAATTACTTTATTTCTTATAATAAGTAATGTA
GTTAGGAGAGGATTAAAACT

Downstream 100 bases:

>100_bases
AGTAAAAAAGACCGCATAGCCCAAGATTGGCTAAGCGGTCTTTTTTGTAGTTTTATTTATTATCCAAAGCTGCACCAACA
AAGTCTTTAAACAAGCTTTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKKLVKSAVVFASLAFIGASATMITENASAASIDTVQKVDDQSIYIPKGIRDGTATEEHDGFEDETNSVLKEVPLLRAAG
YPDVNSYIKTNKFSTASIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQ
IHPTENGVWGAGQYANARFIQVELVRSKTFDEFSRSINNYAYYTAYLLDQYKLPVDSAHSDGKGTVWSHDAVTRYLGGTT
HTDPVGYFNQWGYNFNSFVTLVNEKYKAIQSSKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGINPLSVYVG
KNMRILREAKTPITTWYQFSIDGKTIGWVDTRALDTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGTLSTYK
NQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLTTVSPVDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGVNPL
SVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALNTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGT
LSTYKNQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLSTSK

Sequences:

>Translated_604_residues
MKKLVKSAVVFASLAFIGASATMITENASAASIDTVQKVDDQSIYIPKGIRDGTATEEHDGFEDETNSVLKEVPLLRAAG
YPDVNSYIKTNKFSTASIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQ
IHPTENGVWGAGQYANARFIQVELVRSKTFDEFSRSINNYAYYTAYLLDQYKLPVDSAHSDGKGTVWSHDAVTRYLGGTT
HTDPVGYFNQWGYNFNSFVTLVNEKYKAIQSSKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGINPLSVYVG
KNMRILREAKTPITTWYQFSIDGKTIGWVDTRALDTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGTLSTYK
NQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLTTVSPVDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGVNPL
SVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALNTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGT
LSTYKNQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLSTSK
>Mature_604_residues
MKKLVKSAVVFASLAFIGASATMITENASAASIDTVQKVDDQSIYIPKGIRDGTATEEHDGFEDETNSVLKEVPLLRAAG
YPDVNSYIKTNKFSTASIEKQLKSQFPKFNYRNGYGKPEGIVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQ
IHPTENGVWGAGQYANARFIQVELVRSKTFDEFSRSINNYAYYTAYLLDQYKLPVDSAHSDGKGTVWSHDAVTRYLGGTT
HTDPVGYFNQWGYNFNSFVTLVNEKYKAIQSSKVTYDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGINPLSVYVG
KNMRILREAKTPITTWYQFSIDGKTIGWVDTRALDTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGTLSTYK
NQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLTTVSPVDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGVNPL
SVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALNTFYKQSMEKPANLTRYVLANKANEAYYKVPVADSAVKWGT
LSTYKNQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANLSTSK

Specific function: Mediates the entry of Listeria monocytogenes into cells [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 7 LRR (leucine-rich) repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014755
- InterPro:   IPR014756
- InterPro:   IPR001611
- InterPro:   IPR013378
- InterPro:   IPR012569 [H]

Pfam domain/function: PF09479 Flg_new; PF00560 LRR_1; PF08191 LRR_adjacent [H]

EC number: NA

Molecular weight: Translated: 67793; Mature: 67793

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVKSAVVFASLAFIGASATMITENASAASIDTVQKVDDQSIYIPKGIRDGTATEEHD
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCC
GFEDETNSVLKEVPLLRAAGYPDVNSYIKTNKFSTASIEKQLKSQFPKFNYRNGYGKPEG
CCCHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
IVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQIHPTENGVWGAGQYANARFI
EEEEECCCCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE
QVELVRSKTFDEFSRSINNYAYYTAYLLDQYKLPVDSAHSDGKGTVWSHDAVTRYLGGTT
EEEEEHHCCHHHHHHHHCCEEEEEEEEEHHHCCCCCCCCCCCCCCEECHHHHHHHHCCCC
HTDPVGYFNQWGYNFNSFVTLVNEKYKAIQSSKVTYDKIEYDKGVTAYARVKTAPGNAVW
CCCCCCCHHHCCCCHHHEEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCEE
TKPYKTEGAKGINPLSVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALDTFYKQ
CCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEEEECCCEEEEEEHHHHHHHHHH
SMEKPANLTRYVLANKANEAYYKVPVADSAVKWGTLSTYKNQKLTVDRQATIEGQLWYRI
HHCCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCCCCEEEEECCEEECCEEEEEE
KTSSTFIGWTKAANLTTVSPVDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGVNPL
ECCCEEEEEECCCCEEEECCHHHHHCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCE
SVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALNTFYKQSMEKPANLTRYVLAN
EEEECCCEEEEECCCCCEEEEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEC
KANEAYYKVPVADSAVKWGTLSTYKNQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANL
CCCCCEEEEECCCCCEECCCCCCCCCCEEEEECCEEECCEEEEEEECCCEEEEEEEECCC
STSK
CCCC
>Mature Secondary Structure
MKKLVKSAVVFASLAFIGASATMITENASAASIDTVQKVDDQSIYIPKGIRDGTATEEHD
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCC
GFEDETNSVLKEVPLLRAAGYPDVNSYIKTNKFSTASIEKQLKSQFPKFNYRNGYGKPEG
CCCHHHHHHHHHCCHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
IVIHETANNSSTITGEINYMSTNYNNAFVHAFVDKSRIIQIHPTENGVWGAGQYANARFI
EEEEECCCCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE
QVELVRSKTFDEFSRSINNYAYYTAYLLDQYKLPVDSAHSDGKGTVWSHDAVTRYLGGTT
EEEEEHHCCHHHHHHHHCCEEEEEEEEEHHHCCCCCCCCCCCCCCEECHHHHHHHHCCCC
HTDPVGYFNQWGYNFNSFVTLVNEKYKAIQSSKVTYDKIEYDKGVTAYARVKTAPGNAVW
CCCCCCCHHHCCCCHHHEEEEHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCEE
TKPYKTEGAKGINPLSVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALDTFYKQ
CCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEEEECCCEEEEEEHHHHHHHHHH
SMEKPANLTRYVLANKANEAYYKVPVADSAVKWGTLSTYKNQKLTVDRQATIEGQLWYRI
HHCCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCCCCEEEEECCEEECCEEEEEE
KTSSTFIGWTKAANLTTVSPVDKIEYDKGVTAYARVKTAPGNAVWTKPYKTEGAKGVNPL
ECCCEEEEEECCCCEEEECCHHHHHCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCE
SVYVGKNMRILREAKTPITTWYQFSIDGKTIGWVDTRALNTFYKQSMEKPANLTRYVLAN
EEEECCCEEEEECCCCCEEEEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEC
KANEAYYKVPVADSAVKWGTLSTYKNQKLTVDRQATIEGQLWYRIKTSSTFIGWTKAANL
CCCCCEEEEECCCCCEECCCCCCCCCCEEEEECCEEECCEEEEEEECCCEEEEEEEECCC
STSK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1905979; 11679669; 10635330 [H]