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The map label for this gene is gloA [H]

Identifier: 116873600

GI number: 116873600

Start: 2216393

End: 2216782

Strand: Direct

Name: gloA [H]

Synonym: lwe2184

Alternate gene names: 116873600

Gene position: 2216393-2216782 (Clockwise)

Preceding gene: 116873589

Following gene: 116873601

Centisome position: 78.76

GC content: 35.38

Gene sequence:

>390_bases
ATGACTACAAAAATGTTACATACATGTATTCGCGTAAAAAACCTAACAGAATCCATTGCTTTTTATGAAAAAGCATTAGG
ACTAAAAGAGGTTCGTCGTAAAGACTTTCCAGATTTTGAGTTTACTCTAGTTTATATGGCGTTTGAAGAAGGCGGCTTTG
AATTAGAACTTACTTATAATTATGACCAAAAAGAAGCTTACGATTTAGGAAATGGTTATGGTCATTTAGCTGTTGGGGTT
CCTGATGTCCGCTCCCTTCTAAAAGAACACCAAGAAGCTGGCTATACAGTAACAGATTTAAAAGGACTTCCGGGTGAAGA
TCCATTCTATTATTTCCTTACAGATCCAGATGGTTATAAAACAGAAATCATTCAAGATGGCGCTTTATAA

Upstream 100 bases:

>100_bases
TATACCGAAAAAGAAGCTATACTGCCATTAATATTCCTTGTTTGTATTCACTTCCGAGATATACTACAATGAAGAAAGAA
GTTTAAAAGGAGGAATAAAA

Downstream 100 bases:

>100_bases
TTAGAAATAGAACTGGAGACTTTTAATGATAAAATATTTCATTATTATTTTAATCGCACTAGCAATTAATGGGATTAGTT
TTCTATTTCATAATGATATA

Product: lactoylglutathione lyase, putative

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MTTKMLHTCIRVKNLTESIAFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGV
PDVRSLLKEHQEAGYTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL

Sequences:

>Translated_129_residues
MTTKMLHTCIRVKNLTESIAFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGV
PDVRSLLKEHQEAGYTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL
>Mature_128_residues
TTKMLHTCIRVKNLTESIAFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGVP
DVRSLLKEHQEAGYTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=156, Percent_Identity=30.1282051282051, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=35.7142857142857, Blast_Score=82, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=147, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14767; Mature: 14636

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKMLHTCIRVKNLTESIAFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYN
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEC
YDQKEAYDLGNGYGHLAVGVPDVRSLLKEHQEAGYTVTDLKGLPGEDPFYYFLTDPDGYK
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCE
TEIIQDGAL
EEEEECCCC
>Mature Secondary Structure 
TTKMLHTCIRVKNLTESIAFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYN
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEC
YDQKEAYDLGNGYGHLAVGVPDVRSLLKEHQEAGYTVTDLKGLPGEDPFYYFLTDPDGYK
CCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCE
TEIIQDGAL
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]