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The map label for this gene is leuB [H]

Identifier: 116873423

GI number: 116873423

Start: 2035350

End: 2036402

Strand: Direct

Name: leuB [H]

Synonym: lwe2007

Alternate gene names: 116873423

Gene position: 2035350-2036402 (Clockwise)

Preceding gene: 116873422

Following gene: 116873424

Centisome position: 72.33

GC content: 42.07

Gene sequence:

>1053_bases
GTGACATATAAAATTACTTCCTTAGCTGGCGACGGAATTGGTCCAGAAATCATGTCTGCTGGAATCCAAGTATTGGAGTT
AATTGCAAAAAAATACCACCATACTTTTGAAATTAAAGCGCATCCGTTTGGTGGTGCAGGAATTGATGCTACTGGCAGTC
CGCTCCCTCCCGCAACCTTAAAAGCATGTCAAGATGCAGACGCCATTTTACTTGGAGCAATTGGCGGTCCTAAATGGGAT
AATGCAACGAAGCGTCCCGAAGATGGCTTACTTGCTCTACGAAAAGCACTTGGCCTTTTCGCTAATATCCGCCCTATCCA
AGTTCCAAGTGCAATCAGCCACCTTTCTCCTTTAAAAAAGGAAATTGTTGAGCATACAGATTTCGTGGTTGTTCGTGAGC
TAACTGGGGGTCTTTACTTCGGGGAACCAAAACACTGGAATGACGAGTGCGCTATTGATTCTTTGACGTATACTCGTGCA
GAAATCGAGCGAATTATTGAGAAAGCGTTTGAAATCGCTGCAACAAGAAATAAAAAAGTAACTTCTGTAGATAAAGCAAA
TGTTCTTGCTTCTAGTAAATTATGGCGTAAAATCGCCGAAGAAGTAGCTAGTCGTCATCCAGCTATTACGCTAGAACATT
TATACGTAGATGCCGCTGCAATGATAATGATTCAGCGACCAACTACATTCGATGTTATTGTGACAGAAAATTTATTTGGA
GATATTTTAAGTGATGAAGCTTCTGTTATCACAGGTTCACTGGGGATGCTCCCTTCTGCTAGTCATGCGGAAAATGGGCC
GTCTTTATATGAACCAATTCACGGTTCTGCGCCAGATATCGCCAACCAAAATATCGCCAATCCAATGTCGATGATTTCTT
CTGTGTCAATGATGCTCCGCCAATCCTTTTCCCTTTTTAAAGAAGCGGATGCGATTGATGCGGCTGCAACGAGAACTATG
AAAGCTGGATTTTTAACCGCTGATCTTGGAGGCAATACTTCTACAACGGATTTTACAAGCGAAGTTCTAAAACAAATTGA
AGGAGGAGAATAA

Upstream 100 bases:

>100_bases
GACTTTGATGTTTTAACGGCTAGTGCTAAAGCTTATTTACAAGCTTCAGGAAAAAGTAAAAGCACTACAAAGCAAGTTGA
TTTTGAGGAGGTAAAATAAC

Downstream 100 bases:

>100_bases
AATGGGAAAAACGCTTTTTGATAAACTTTGGAATCGTCATGTCATTTATGGCAAAGAAGGTGAACCGCAATTATTATATG
TTGATCTTCATTTGATTCAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTYKITSLAGDGIGPEIMSAGIQVLELIAKKYHHTFEIKAHPFGGAGIDATGSPLPPATLKACQDADAILLGAIGGPKWD
NATKRPEDGLLALRKALGLFANIRPIQVPSAISHLSPLKKEIVEHTDFVVVRELTGGLYFGEPKHWNDECAIDSLTYTRA
EIERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIAEEVASRHPAITLEHLYVDAAAMIMIQRPTTFDVIVTENLFG
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFKEADAIDAAATRTM
KAGFLTADLGGNTSTTDFTSEVLKQIEGGE

Sequences:

>Translated_350_residues
MTYKITSLAGDGIGPEIMSAGIQVLELIAKKYHHTFEIKAHPFGGAGIDATGSPLPPATLKACQDADAILLGAIGGPKWD
NATKRPEDGLLALRKALGLFANIRPIQVPSAISHLSPLKKEIVEHTDFVVVRELTGGLYFGEPKHWNDECAIDSLTYTRA
EIERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIAEEVASRHPAITLEHLYVDAAAMIMIQRPTTFDVIVTENLFG
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFKEADAIDAAATRTM
KAGFLTADLGGNTSTTDFTSEVLKQIEGGE
>Mature_349_residues
TYKITSLAGDGIGPEIMSAGIQVLELIAKKYHHTFEIKAHPFGGAGIDATGSPLPPATLKACQDADAILLGAIGGPKWDN
ATKRPEDGLLALRKALGLFANIRPIQVPSAISHLSPLKKEIVEHTDFVVVRELTGGLYFGEPKHWNDECAIDSLTYTRAE
IERIIEKAFEIAATRNKKVTSVDKANVLASSKLWRKIAEEVASRHPAITLEHLYVDAAAMIMIQRPTTFDVIVTENLFGD
ILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLRQSFSLFKEADAIDAAATRTMK
AGFLTADLGGNTSTTDFTSEVLKQIEGGE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=36.0230547550432, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI28178821, Length=355, Percent_Identity=28.7323943661972, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI28178816, Length=351, Percent_Identity=28.7749287749288, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4758582, Length=261, Percent_Identity=31.8007662835249, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI28178819, Length=192, Percent_Identity=32.2916666666667, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178838, Length=233, Percent_Identity=32.618025751073, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=46.8926553672316, Blast_Score=320, Evalue=6e-89,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=33.2402234636872, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787381, Length=384, Percent_Identity=25, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=32.7683615819209, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.8591549295775, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=370, Percent_Identity=28.9189189189189, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17505779, Length=358, Percent_Identity=28.2122905027933, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=356, Percent_Identity=46.9101123595506, Blast_Score=298, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6322097, Length=367, Percent_Identity=35.6948228882834, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=33.7078651685393, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=354, Percent_Identity=30.5084745762712, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643270, Length=351, Percent_Identity=35.8974358974359, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24643268, Length=351, Percent_Identity=35.8974358974359, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=30.4225352112676, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=27.9538904899135, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=27.9538904899135, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161078633, Length=354, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24650122, Length=354, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078635, Length=354, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI161078637, Length=354, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161078639, Length=349, Percent_Identity=27.2206303724928, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130355, Length=351, Percent_Identity=23.3618233618234, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37596; Mature: 37465

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKITSLAGDGIGPEIMSAGIQVLELIAKKYHHTFEIKAHPFGGAGIDATGSPLPPATL
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHH
KACQDADAILLGAIGGPKWDNATKRPEDGLLALRKALGLFANIRPIQVPSAISHLSPLKK
HHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EIVEHTDFVVVRELTGGLYFGEPKHWNDECAIDSLTYTRAEIERIIEKAFEIAATRNKKV
HHHHHCCEEEEEEHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSVDKANVLASSKLWRKIAEEVASRHPAITLEHLYVDAAAMIMIQRPTTFDVIVTENLFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEEEEECCCEEEEEEECHHHH
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLR
HHHCCCHHHEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QSFSLFKEADAIDAAATRTMKAGFLTADLGGNTSTTDFTSEVLKQIEGGE
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TYKITSLAGDGIGPEIMSAGIQVLELIAKKYHHTFEIKAHPFGGAGIDATGSPLPPATL
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHH
KACQDADAILLGAIGGPKWDNATKRPEDGLLALRKALGLFANIRPIQVPSAISHLSPLKK
HHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EIVEHTDFVVVRELTGGLYFGEPKHWNDECAIDSLTYTRAEIERIIEKAFEIAATRNKKV
HHHHHCCEEEEEEHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSVDKANVLASSKLWRKIAEEVASRHPAITLEHLYVDAAAMIMIQRPTTFDVIVTENLFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEEEEECCCEEEEEEECHHHH
DILSDEASVITGSLGMLPSASHAENGPSLYEPIHGSAPDIANQNIANPMSMISSVSMMLR
HHHCCCHHHEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QSFSLFKEADAIDAAATRTMKAGFLTADLGGNTSTTDFTSEVLKQIEGGE
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]