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The map label for this gene is ilvB [H]

Identifier: 116873419

GI number: 116873419

Start: 2030345

End: 2032066

Strand: Direct

Name: ilvB [H]

Synonym: lwe2003

Alternate gene names: 116873419

Gene position: 2030345-2032066 (Clockwise)

Preceding gene: 116873418

Following gene: 116873420

Centisome position: 72.15

GC content: 40.24

Gene sequence:

>1722_bases
GTGATTGATACGACAAAAACAAATGAAAAAGCGACAAAAAAGGTAAATAAAAGTGGCGCAGAGCTGTTAATTGACTCCTT
GAAAAAGCAACAAGTAGACATGATTTTTGGTTATCCAGGTGGCGCTGTTCTACCTCTCTATGATGCTTTTTATGACTGTG
ATATTCCCCATATTCTAACTAGGCACGAACAAGGAGCCATTCATGCTGCGGAAGGTTATGCTCGTGTAACTGGAAAACCA
GGTGTTGTTGTTGTAACGAGTGGACCTGGTGCAACGAATGTATTAACTGGTATAGCAGATGCAATGAGCGATTCAATTCC
GCTAGTTATTTTCACTGGACAAGTTCATACACCTGGAATTGGAAAAGATGCTTTCCAAGAAGCTGATATGATTGGGCTTA
CAATTCCAATCACCAAGTATAACTATCAAGTTCGCGATGTACGAGATTTGCCAAAAATCGTCAATGAAGCTTTTCATATC
GCAAGTACTGGCCGAAAAGGACCTGTCGTCGTGGATATTCCAAAAGATATGGGCATTATCCAAACAGATACTGTTCGTCC
AGATACGATAGATTTGCCGGGTTATCAACCGACTTATTCACCGAACCCACTTCAGCTCGAAAAATTAATGCAAGCTCTTT
CTGCCTCAAGCAAGCCACTTATTCTTGCAGGTGCTGGAGTCAATCATTCTAGAGCTACAGCTGAATTACTCGAATTTGCT
GAACGTTATCAAATTCCAATTGTTAATACACTCCTCGGCCTTGGAAGTTTTCCGCAAAGTCATGATTTATTCTTAGGTAT
GGGAGGAATGCACGGCTCTTATGCAGCAAATATGGCATTGACAGATTGCGATTTACTCATTAATTTTGGCTCAAGATTTG
ATGATCGTCTTGCAAGTGCGCCAAAAGAATTTGCCACTAAAGCAACTATTGCTCATATCGATATTGACCCTGCTGAAATT
GGCAAAATTATTGAAACACAAATTCCAATCGTAGCTGACATTAATGAAACGCTTACTCAACTACTACAGATGGAACTCCC
GGTTTATCCAGATACATCTCATTGGTACAAATTAAATATGACACGCAAAAATCGCCATCCTTTCCATTTTGACAAAACAA
AAAAAGCAGAAATTAAACCACAGCGTGTGATTGAACTCATTGGCGAAATCACACAAGGTGAGGCGCTTGTTTCCACTGAT
GTAGGACAACATCAAATGTGGACAGCGCAGTTCTACCCTTTCCAATTTGATCACCAAATTATTACAAGTGGTGGTCTTGG
CACAATGGGCTTTGGTTTTCCAGCAGCGGTTGGTGCACAACTTGCTTTCCCAGAAAAGACTGTAGTCGCAGTTGTTGGCG
ACGGCGGATTTCAAATGACCAACCAAGAATTAGCGATTTTAAACGATTATCAAATCAATGTTAAAACAGTTATTATCAAT
AATGGTTCGCTTGGAATGGTTCGTCAGTGGCAAGAGAAATTCCATGGTGAAAGATATTCTCATTCAATTTTTACAAGCCA
ACCAGACTTTGTTAAATTATCTGAAGCATATGGTGTCAAAGGTGTTCGTTTGACGAATCCTGAAACGCTTGAAAAAGATT
TAAGAAAAGCATTCGCTCATCCGGGTCCAATTGTTATTGATGTTCATGTCGCAAAAGACGAACTTGTACTTCCAATGGTT
CCAAGCGGCAAACCAAATCACCAAATGGAAGGGGTGGAATAA

Upstream 100 bases:

>100_bases
GTTATCTTGCTAGATATACCGCTCTTGTAACTAGCGCACATACTGGTGGTATTTTGCAAATCCCAGAAGATTTAATCGAC
TAAAAAATGAGGTGATAAGC

Downstream 100 bases:

>100_bases
ATGCGCCGAATCATTACTGCTACTGTGAACAACTCTTCTGGCGTACTTAATCGTATTACTGGGGTTATCTCTAGACGCCA
ATACAATATTGATAGTATCT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIDTTKTNEKATKKVNKSGAELLIDSLKKQQVDMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTDTVRPDTIDLPGYQPTYSPNPLQLEKLMQALSASSKPLILAGAGVNHSRATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVYPDTSHWYKLNMTRKNRHPFHFDKTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWTAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPEKTVVAVVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE

Sequences:

>Translated_573_residues
MIDTTKTNEKATKKVNKSGAELLIDSLKKQQVDMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTDTVRPDTIDLPGYQPTYSPNPLQLEKLMQALSASSKPLILAGAGVNHSRATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVYPDTSHWYKLNMTRKNRHPFHFDKTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWTAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPEKTVVAVVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE
>Mature_573_residues
MIDTTKTNEKATKKVNKSGAELLIDSLKKQQVDMIFGYPGGAVLPLYDAFYDCDIPHILTRHEQGAIHAAEGYARVTGKP
GVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGIGKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHI
ASTGRKGPVVVDIPKDMGIIQTDTVRPDTIDLPGYQPTYSPNPLQLEKLMQALSASSKPLILAGAGVNHSRATAELLEFA
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASAPKEFATKATIAHIDIDPAEI
GKIIETQIPIVADINETLTQLLQMELPVYPDTSHWYKLNMTRKNRHPFHFDKTKKAEIKPQRVIELIGEITQGEALVSTD
VGQHQMWTAQFYPFQFDHQIITSGGLGTMGFGFPAAVGAQLAFPEKTVVAVVGDGGFQMTNQELAILNDYQINVKTVIIN
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAHPGPIVIDVHVAKDELVLPMV
PSGKPNHQMEGVE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=24.7330960854093, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=23.489932885906, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=44.4244604316547, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=40.7342657342657, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=33.3333333333333, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=28.4671532846715, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=23.6598890942699, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=26.8206039076377, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=26.8206039076377, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=579, Percent_Identity=24.1796200345423, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=43.205574912892, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=496, Percent_Identity=24.5967741935484, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=483, Percent_Identity=23.1884057971014, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=482, Percent_Identity=23.2365145228216, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=487, Percent_Identity=22.3819301848049, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=550, Percent_Identity=25.8181818181818, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62719; Mature: 62719

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTTKTNEKATKKVNKSGAELLIDSLKKQQVDMIFGYPGGAVLPLYDAFYDCDIPHILT
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHCCCCCHHHH
RHEQGAIHAAEGYARVTGKPGVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGI
CCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHIASTGRKGPVVVDIPKDMGII
CHHHHHCCCEEEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEE
QTDTVRPDTIDLPGYQPTYSPNPLQLEKLMQALSASSKPLILAGAGVNHSRATAELLEFA
EECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASA
HHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHEEECCCCHHHHHHCC
PKEFATKATIAHIDIDPAEIGKIIETQIPIVADINETLTQLLQMELPVYPDTSHWYKLNM
CHHHHCCEEEEEEECCHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEE
TRKNRHPFHFDKTKKAEIKPQRVIELIGEITQGEALVSTDVGQHQMWTAQFYPFQFDHQI
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCEEECCEE
ITSGGLGTMGFGFPAAVGAQLAFPEKTVVAVVGDGGFQMTNQELAILNDYQINVKTVIIN
EECCCCCCCCCCCHHHHCCEEECCCCEEEEEEECCCCEECCCCEEEEECEEEEEEEEEEE
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAH
CCCCCHHHHHHHHHCCCCCCCCEECCCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHHCC
PGPIVIDVHVAKDELVLPMVPSGKPNHQMEGVE
CCCEEEEEEECCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIDTTKTNEKATKKVNKSGAELLIDSLKKQQVDMIFGYPGGAVLPLYDAFYDCDIPHILT
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHCCCCCHHHH
RHEQGAIHAAEGYARVTGKPGVVVVTSGPGATNVLTGIADAMSDSIPLVIFTGQVHTPGI
CCCCCCEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
GKDAFQEADMIGLTIPITKYNYQVRDVRDLPKIVNEAFHIASTGRKGPVVVDIPKDMGII
CHHHHHCCCEEEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEE
QTDTVRPDTIDLPGYQPTYSPNPLQLEKLMQALSASSKPLILAGAGVNHSRATAELLEFA
EECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
ERYQIPIVNTLLGLGSFPQSHDLFLGMGGMHGSYAANMALTDCDLLINFGSRFDDRLASA
HHCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHEEECCCCHHHHHHCC
PKEFATKATIAHIDIDPAEIGKIIETQIPIVADINETLTQLLQMELPVYPDTSHWYKLNM
CHHHHCCEEEEEEECCHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEE
TRKNRHPFHFDKTKKAEIKPQRVIELIGEITQGEALVSTDVGQHQMWTAQFYPFQFDHQI
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCEEECCEE
ITSGGLGTMGFGFPAAVGAQLAFPEKTVVAVVGDGGFQMTNQELAILNDYQINVKTVIIN
EECCCCCCCCCCCHHHHCCEEECCCCEEEEEEECCCCEECCCCEEEEECEEEEEEEEEEE
NGSLGMVRQWQEKFHGERYSHSIFTSQPDFVKLSEAYGVKGVRLTNPETLEKDLRKAFAH
CCCCCHHHHHHHHHCCCCCCCCEECCCCCEEEEHHHCCCCCEEECCHHHHHHHHHHHHCC
PGPIVIDVHVAKDELVLPMVPSGKPNHQMEGVE
CCCEEEEEEECCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]