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The map label for this gene is pfl [H]

Identifier: 116873359

GI number: 116873359

Start: 1970578

End: 1972857

Strand: Direct

Name: pfl [H]

Synonym: lwe1943

Alternate gene names: 116873359

Gene position: 1970578-1972857 (Clockwise)

Preceding gene: 116873352

Following gene: 116873386

Centisome position: 70.02

GC content: 36.62

Gene sequence:

>2280_bases
ATGACAAAAGCATGGGATGGTTTTAAAGGAACCGCATGGCAAGAAAATATTAGTGTAGGACAATTCGTTCAAGATAACTA
CACTCCTTACGATGGTGATGAAAGCTTTTTAGAAAAAAGCACACCAAGAACAACGAAACTAAATGAAAAAATAAATAAAC
TTGTAGAAGAAATGGATGCAAAAGGTGGCGTTCTTGATATGGATAACGCTATCGTATCCAATGTTGCTTCTCATAAAGCA
GGTTACGTAGACCAAGAAAATGAGGTCATCGTTGGTTTACAAACAGACAAACCTTTCAAACTAGCATTTATGCCAAATGG
TGGTCTTAGAACTGCAGAACAATGTTTAACAGACAATGGCTATTCTATAGACCAAGAACTACATGACTTTTATGTAAAAA
ACCGTTCTACTGCAAATGATGGTATTTTTAGAGCTTATACAGATGATATTAAACGTGCGCGTCACTCGCATATCGTTAGT
GGTCTTCCAGATGCCTATTCTCGCGGAAGAATCATCGGCTTATATCAAAAACCAGCTCTTTACGGTGTAGATCGTTTAAT
CGCCGAAAAACAACAAGACTTGAAAAAAATTGCGATTTCTTCGGACGAAAACATTCGCTTACGTGAAGAAATTTGGTTAC
AAATCAAAGCACTTAAAGACTTAATCGTTTTAGGTAATGAATATGGTTTAGAACTTGATCGTCCAGCTGAAAATGCTACA
GAAGCTGTACAATGGACTTACATGGGTTACCTTGCATCTATCAAACAAGCAAACGGTGCTGCAAGCTCTTTTGGTCGTAT
TCCAATTTTCCTTGATATTTATATCCAACGTGATTTAGAAAAAGGAATTATCACAGAATTCGATGCGCAAGAATTAATCG
AACAATTAACCCTTAAATTAAGAATGGTTCGTTTTGCAAGAACAGATGGTTACAATGAACTTTATGCTTCCAACCCAACA
TTCGTAACAACTTCTATGGCTGGTATGGGTGCAGACGGTCGTCATCGTGTAACTAAAACAGATTATCGTTTCTTACACTG
CCTAGACAACTTAGGAAACTCCGCGGAACCTAACTTAACCGTCCTTTGGGATGCTCGTTTACCTGAAAGTTTTAAAGAAT
ATTGTATGAAAATGAGCGTGAAACATTCCTCTATTCAATATGAAAATGATAAATTAATGCAAGAAGAAGGTTACGGGGAT
ATGCAATGTATCAGCTGTTGTGTTAGTCCACTTAACCCAGAAGCTGACAAAGACAAAGGCGAAACCCACAACCTGCAATA
TTTTGGTGCTCGTGTTAATGTGCTTAAATGTCTTCTTGGTGCAATCAACGGTGGTAAAGATGATCTTCATAAAAACCAAG
TATTCGATGTTGTAGAACCAATTACAACTGAATACCTTGAATACGAAGAAGTACTAGAAAAATTCGATAAATCAATGGAT
TGGTTAACAGACACATACGTTGATGCTATGAATATTATTCACTTTATGACAGACAAATACAATTACGAAAGCATGCAAAT
GGCCTTCTTACCATCAAAAGTAACAGCCAACATGGGCTTTGGTATTTGTGGATTCGCTAATGTAGTAGATAGTTTAAGTG
CGATTAAGCATGCAAAAGTAAAAACTATTCGTGACGAAGATGGCTTTGTCTATGATTATGAAGTAGAAGGCGACTTCCCT
CGTTACGGTGAAAATGATGACCGTGCAGATGATATCGCTGTAATGGTTCTAAAAATGTTCAAAGACAAACTTGACTCCCA
CAAACTTTACAAAGATAGTGAAGCAACTGTTTCTGTTCTAACAATCACTTCAAACGTTGCTTATTCTAAACAATGTGGTA
ACTCCCCAGTGCATAAAGGACCTGTATTTGATGAAAATGGAAAAATCGTCAAAGAACCAGAATTCTTCAGTCCTGGAGCT
AACCCTTCTAACAAAGCAAAAGGTGGTTTCTTAGATAACCTTGCTAGTCTTTCTAAATTACCGTTCCATTATGCAAATGA
CGGAATTTCCTTAACTATCCAAGGAGCTCCAAAAATGTTCGGTAAAACAACAGAAGAACAACATCACAATCTCGTTGGTA
TTCTAGATGGTTACTTTACAAAAGGCGGTCAACATATTAACTTAAATGTTCTTAACCACGACGAAGTAATCGAAAAAATC
AAAGCTGGTATTCCAGTAATCTTGCGTATCAGTGGTTACTGCTTAAATACAAAAGACTTAAATGAAGAACAAAAAATGGA
ACTATGCCAAAGAATGTTCCACGAAAAATTAATCGGTTAA

Upstream 100 bases:

>100_bases
TTCATTTTCACAAAGGAGTATGCTGTTCAATGAAGAAAGATTCTAAGAACAAGTTTCTGTTTTTAAGTGAAAAGAAACAC
AAATGAAGGAGGAAGAAATA

Downstream 100 bases:

>100_bases
TAAGAAAAAGAACCAAATCTGCGAAAAAACAGATTTGGTTCTTTTTTTATGCATTCGATACTTCCGCCGCATAGGCTCCT
GCCGTATGTCCAGTCACTAG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKA
GYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVS
GLPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELDRPAENAT
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPT
FVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGD
MQCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFP
RYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGA
NPSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG

Sequences:

>Translated_759_residues
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKA
GYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVS
GLPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELDRPAENAT
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPT
FVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGD
MQCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFP
RYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGA
NPSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
>Mature_758_residues
TKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDAKGGVLDMDNAIVSNVASHKAG
YVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNGYSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSG
LPDAYSRGRIIGLYQKPALYGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELDRPAENATE
AVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKLRMVRFARTDGYNELYASNPTF
VTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLTVLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDM
QCISCCVSPLNPEADKDKGETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMDW
LTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKVKTIRDEDGFVYDYEVEGDFPR
YGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVLTITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGAN
PSNKAKGGFLDNLASLSKLPFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKIK
AGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=773, Percent_Identity=44.372574385511, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=769, Percent_Identity=42.5227568270481, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=596, Percent_Identity=20.3020134228188, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85566; Mature: 85435

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDA
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCC
KGGVLDMDNAIVSNVASHKAGYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNG
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCCC
YSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSGLPDAYSRGRIIGLYQKPAL
CCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHH
YGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELDRPAENAT
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHH
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKL
HHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHH
RMVRFARTDGYNELYASNPTFVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLT
HHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDMQCISCCVSPLNPEADKDKG
EEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
ETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
CCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIRDEDGFVYDYEVEGDFPRYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVL
HEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGANPSNKAKGGFLDNLASLSKL
EEECCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHC
PFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
CCEEECCCEEEEECCCCHHHCCCCHHHHCCEEEEEHHHHCCCCCEEEEEEECHHHHHHHH
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
HCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKAWDGFKGTAWQENISVGQFVQDNYTPYDGDESFLEKSTPRTTKLNEKINKLVEEMDA
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCC
KGGVLDMDNAIVSNVASHKAGYVDQENEVIVGLQTDKPFKLAFMPNGGLRTAEQCLTDNG
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCCC
YSIDQELHDFYVKNRSTANDGIFRAYTDDIKRARHSHIVSGLPDAYSRGRIIGLYQKPAL
CCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCHH
YGVDRLIAEKQQDLKKIAISSDENIRLREEIWLQIKALKDLIVLGNEYGLELDRPAENAT
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHH
EAVQWTYMGYLASIKQANGAASSFGRIPIFLDIYIQRDLEKGIITEFDAQELIEQLTLKL
HHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHH
RMVRFARTDGYNELYASNPTFVTTSMAGMGADGRHRVTKTDYRFLHCLDNLGNSAEPNLT
HHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLWDARLPESFKEYCMKMSVKHSSIQYENDKLMQEEGYGDMQCISCCVSPLNPEADKDKG
EEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
ETHNLQYFGARVNVLKCLLGAINGGKDDLHKNQVFDVVEPITTEYLEYEEVLEKFDKSMD
CCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLTDTYVDAMNIIHFMTDKYNYESMQMAFLPSKVTANMGFGICGFANVVDSLSAIKHAKV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KTIRDEDGFVYDYEVEGDFPRYGENDDRADDIAVMVLKMFKDKLDSHKLYKDSEATVSVL
HEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TITSNVAYSKQCGNSPVHKGPVFDENGKIVKEPEFFSPGANPSNKAKGGFLDNLASLSKL
EEECCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHC
PFHYANDGISLTIQGAPKMFGKTTEEQHHNLVGILDGYFTKGGQHINLNVLNHDEVIEKI
CCEEECCCEEEEECCCCHHHCCCCHHHHCCEEEEEHHHHCCCCCEEEEEEECHHHHHHHH
KAGIPVILRISGYCLNTKDLNEEQKMELCQRMFHEKLIG
HCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550181; 12397186 [H]