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The map label for this gene is pflC [H]

Identifier: 116872839

GI number: 116872839

Start: 1417012

End: 1417758

Strand: Direct

Name: pflC [H]

Synonym: lwe1423

Alternate gene names: 116872839

Gene position: 1417012-1417758 (Clockwise)

Preceding gene: 116872838

Following gene: 116872841

Centisome position: 50.35

GC content: 36.14

Gene sequence:

>747_bases
ATGACAGAGGTTTTAGGAAGAGTTCATTCAGTAGAAACAATGGGAACAGTAGACGGTCCAGGTATCCGTTTTATCGTTTT
TATGCAGGGGTGTTTGCTTCGTTGCCAATTTTGTCATAATCCCGATACTTGGAAAATTGGTACTGGCACAGAACGTTCTG
CGCAAAATGTATTCAACGAAGCGATTAAATATAAAGAGTTTTGGGATGCATCAGGTGGTGGTGTTACAGTTAGTGGTGGT
GAACCTTTACTTCAAGTAGATTTCCTAATTGAGTTTTTCACGCTATGTAAAAAAGCAGGTGTCCATACAACAATCGACTC
TTGTGGTGGTTGTTTCACACGTGATCCTGAGTTTATCGAGAAATTGGACCGATTGATGGAAGTAACAGATTTAATTTTAC
TTGATATTAAACAAATTAACCCAGAAAAACATTTAAAACTGACTACAAAATCTAATGCACCAATTATTGATTTTGCTCAC
TATTTGCGTGATAAAGAGCAACCGATTTGGATTAGACATGTACTAATTCCAACTAAAACCGATGATCCTGAAGATTTAAC
AAAGCTTCATGAATTCATTCAAACACTTCCAAATGTAAAACAAGTGGATGTACTTCCATACCATACAATGGGAGTTTATA
AATGGAAAGAAATGGGCATTCGTTATCCTTTAGAAGGTATCGAAGCACCAGAAGAAGAAGTGGTAGCTTTGGCTAATAAG
ATTTTAGAAACAAGTAGTTATAAATAG

Upstream 100 bases:

>100_bases
CGTACAATGCACGAATCTATGTAATTATAGGCGAGAACGCTTCAACAAGGATTGCTCACCAGTAGCAATCCTTGTTATTT
AGGAAAGGAAGAGGAGAATT

Downstream 100 bases:

>100_bases
TAAAAACCGGCGAATCCTAAATTCGCCGGTTTTTTTATAGTATTTTATACATTTCTTCGCACCATCTTAGTTCTGTTTGA
ATCAAATCTAATCGTCTTGC

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQNVFNEAIKYKEFWDASGGGVTVSGG
EPLLQVDFLIEFFTLCKKAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAH
YLRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
ILETSSYK

Sequences:

>Translated_248_residues
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQNVFNEAIKYKEFWDASGGGVTVSGG
EPLLQVDFLIEFFTLCKKAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAH
YLRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
ILETSSYK
>Mature_247_residues
TEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQNVFNEAIKYKEFWDASGGGVTVSGGE
PLLQVDFLIEFFTLCKKAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHY
LRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANKI
LETSSYK

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=245, Percent_Identity=45.7142857142857, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=24.8175182481752, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI226510931, Length=284, Percent_Identity=23.2394366197183, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28171; Mature: 28040

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQNVFNE
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHH
AIKYKEFWDASGGGVTVSGGEPLLQVDFLIEFFTLCKKAGVHTTIDSCGGCFTRDPEFIE
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHH
KLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHYLRDKEQPIWIRHVLIPTKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
DDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
CCHHHHHHHHHHHHHCCCCCEEECCCCHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ILETSSYK
HHHHCCCC
>Mature Secondary Structure 
TEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQNVFNE
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHH
AIKYKEFWDASGGGVTVSGGEPLLQVDFLIEFFTLCKKAGVHTTIDSCGGCFTRDPEFIE
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHH
KLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHYLRDKEQPIWIRHVLIPTKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
DDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
CCHHHHHHHHHHHHHCCCCCEEECCCCHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ILETSSYK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]