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The map label for this gene is buk [H]

Identifier: 116872801

GI number: 116872801

Start: 1372458

End: 1373525

Strand: Direct

Name: buk [H]

Synonym: lwe1385

Alternate gene names: 116872801

Gene position: 1372458-1373525 (Clockwise)

Preceding gene: 116872800

Following gene: 116872802

Centisome position: 48.77

GC content: 38.3

Gene sequence:

>1068_bases
ATGTCTTTTGATGTTTTGACAATAAATCCTGGTTCCACATCTACAAAACTCGCCGTTTACCAAGGTGATAAAGTACTTTT
TGAAGAAACAGTCAGACACACGATGCAAGAATTCGCCGACTTTAACAATGTACAGGAACAATTTGATTTTAGATGGCAAG
TATTGCGACGAGTGATTGATGCGTTTGGTTATGATGTGAATAATTTAGATGCAGTAGTTGGACGAGGTGGACTTCTTCGA
CCAGTTGCTGGTGGTACATATATGGTTACTGAAAAGATGTTAGCTGATTTAAAAACAAATAAATACGGCGAACATGCTTC
CAACCTTGGAGCCATGCTTGCAAAAAAACTGGCAGATACACTAGATATTCCTAGTTTTATCGTTGATCCAGTTGTAGTGG
ATGAAATGCTACCAATTGCAAGATTTTCTGGAAACGAACTCATTGCAAGAAAAAGTATTTTCCACGCTTTAAACCATAAA
GCAGCAGGAAGAAAGATTGCAAAAAAGTTAGGCTCAGATTATGAGAAGCTGAATTTTGTTATTGCGCATCTTGGCGGAGG
TATTTCGGTTGCTGCACATAGACAAGGTAAAGCAGTGGACGTCAATAATGCCTTAGATGGAGATGGTCCATTTAGCCCTG
AACGATCTGGTTCTCTTCCTATGAATGATTTTTTAGAAGCGTGTTTTAGTGGGAAGTGGAATAAACGTGAACTACATGAT
TTGATTATTGGTCGTGGTGGAATGATTTCTTACTTAGGAACTAATAGCATGTTAGAGGTAGAAGCTAAAGTAAAAGCAGG
AGATGAAAAAGCGATACAAGCTTTTGATGCAATGGCTTATCAAGTTAGTAAAGAAATCGGTGCCTGTTCTACTGTATTAC
ATGGCAAAATTGATGCTATTATTTTAACTGGCGGACTTGCTAGAAGTGACCTTTTCACAAGTAAAATTATCGAACAAACA
AACTGGATTGCCAGTGTGATTATAGAACCTGGAGAAGATGAACTGGAAGCCTTAAATAGCGGTGTACAACGTGTGCTCGC
TGGACTTGAAAAAGAAAAATTGTACTAG

Upstream 100 bases:

>100_bases
ATGCAAAGGCTTTTATTTGATTACAGCCTACGTGTTACAATAGTCCTTGGGAAAATGATAACCTAAAGCATTTTTCACAA
TTAAATTTGGAGGAAATCAA

Downstream 100 bases:

>100_bases
AGGAGGAGATAATGTGGCAACAGAATATGATGTAGTTGTTCTTGGCGGAGGAACTGGCGGTTACGTCGCAGCAATCCAAG
CAGCTAAGAATGGCCAAAAA

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQEFADFNNVQEQFDFRWQVLRRVIDAFGYDVNNLDAVVGRGGLLR
PVAGGTYMVTEKMLADLKTNKYGEHASNLGAMLAKKLADTLDIPSFIVDPVVVDEMLPIARFSGNELIARKSIFHALNHK
AAGRKIAKKLGSDYEKLNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWNKRELHD
LIIGRGGMISYLGTNSMLEVEAKVKAGDEKAIQAFDAMAYQVSKEIGACSTVLHGKIDAIILTGGLARSDLFTSKIIEQT
NWIASVIIEPGEDELEALNSGVQRVLAGLEKEKLY

Sequences:

>Translated_355_residues
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQEFADFNNVQEQFDFRWQVLRRVIDAFGYDVNNLDAVVGRGGLLR
PVAGGTYMVTEKMLADLKTNKYGEHASNLGAMLAKKLADTLDIPSFIVDPVVVDEMLPIARFSGNELIARKSIFHALNHK
AAGRKIAKKLGSDYEKLNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWNKRELHD
LIIGRGGMISYLGTNSMLEVEAKVKAGDEKAIQAFDAMAYQVSKEIGACSTVLHGKIDAIILTGGLARSDLFTSKIIEQT
NWIASVIIEPGEDELEALNSGVQRVLAGLEKEKLY
>Mature_354_residues
SFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQEFADFNNVQEQFDFRWQVLRRVIDAFGYDVNNLDAVVGRGGLLRP
VAGGTYMVTEKMLADLKTNKYGEHASNLGAMLAKKLADTLDIPSFIVDPVVVDEMLPIARFSGNELIARKSIFHALNHKA
AGRKIAKKLGSDYEKLNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWNKRELHDL
IIGRGGMISYLGTNSMLEVEAKVKAGDEKAIQAFDAMAYQVSKEIGACSTVLHGKIDAIILTGGLARSDLFTSKIIEQTN
WIASVIIEPGEDELEALNSGVQRVLAGLEKEKLY

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38767; Mature: 38636

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQEFADFNNVQEQFDFRWQVLRRVID
CCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGYDVNNLDAVVGRGGLLRPVAGGTYMVTEKMLADLKTNKYGEHASNLGAMLAKKLADT
HHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDIPSFIVDPVVVDEMLPIARFSGNELIARKSIFHALNHKAAGRKIAKKLGSDYEKLNFV
HCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWNKRELHD
HHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
LIIGRGGMISYLGTNSMLEVEAKVKAGDEKAIQAFDAMAYQVSKEIGACSTVLHGKIDAI
HHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ILTGGLARSDLFTSKIIEQTNWIASVIIEPGEDELEALNSGVQRVLAGLEKEKLY
EEECCCHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQEFADFNNVQEQFDFRWQVLRRVID
CEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGYDVNNLDAVVGRGGLLRPVAGGTYMVTEKMLADLKTNKYGEHASNLGAMLAKKLADT
HHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LDIPSFIVDPVVVDEMLPIARFSGNELIARKSIFHALNHKAAGRKIAKKLGSDYEKLNFV
HCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWNKRELHD
HHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
LIIGRGGMISYLGTNSMLEVEAKVKAGDEKAIQAFDAMAYQVSKEIGACSTVLHGKIDAI
HHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ILTGGLARSDLFTSKIIEQTNWIASVIIEPGEDELEALNSGVQRVLAGLEKEKLY
EEECCCHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]