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The map label for this gene is arsB [H]

Identifier: 116872731

GI number: 116872731

Start: 1307225

End: 1308520

Strand: Direct

Name: arsB [H]

Synonym: lwe1315

Alternate gene names: 116872731

Gene position: 1307225-1308520 (Clockwise)

Preceding gene: 116872730

Following gene: 116872734

Centisome position: 46.45

GC content: 35.11

Gene sequence:

>1296_bases
ATGCAGATTACTTTAGCTGTTTTAGTATTCGCCATGACACTTTTATTTGTGATTTGGCAACCTAGAAATTTATCAATTGG
ATGGTCAGCTTGTTGTGGAGCATTAATCGCATTATTACTTGGTGTTGTTACATTATCAGATGTTGTCACTGTCACAGGCA
TTGTTTGGAATGCTACACTCGCTTTTATTGCAATTATTATTATTTCGCTTATTTTAGATGAAATTGGATTTTTTGAGTGG
GCTGCATTACATATGGCGAGAATTGCGCGTGGAAATGGATTGCTAATGTTTATACTTATTTCTGTTTTAGGAGCATTTGT
TGCCGCATTTTTTGCTAACGACGGCGCAGCGTTAATTTTGACGCCAATTGTTTTAGCGATGGTGCGTGCGCTTCATTTTG
ATGAGAAAAAGGTGTTTCCATTTATTATTGCAAGTGGATTTATAGCAGATACTACATCTTTACCGCTTGTGGTTAGTAAT
TTAGTAAATATTGTTTCAGCGGATTTCTTCCATATTAGTTTTTCCAAATATGCCTTAATAATGTGGATTCCAACGCTTTT
TTCTTTGATTGCGAGTATTGTGATTTTATATATTTACTTTCGAAAAGCGTTGCCTAGAAAATATGATGCTAGCGATATTC
AAGTACCGTCTTCTGCTCTTAAAGACAAGAAAATGTTTCGTATTTCTTGGGGCATACTTATTGTGCTTGTAATCGGTTAT
TTTTCAAGTAGCTTTTTAGAAATTCCTGTATCATTTATCGCTATACTAGTAGCGCTCATCTTTTTATTGGTTGGTGCAAA
AAGTCACGCAGTATCTACAAAGTCTATTTTAAAAGGGGCACCTTGGAATATTGTATTTTTTTCAGTTGGTATGTATGTGG
TAGTATATGGTTTGCAAAATGCGGGAATTACCAAATTATTATCAGAAGCTATCTCATATGTATCAAATTATGGCCTATTT
ATCGGTACAGTGGGAATGGGGTTTATTGCAGCAATTCTATCTTCTGTGATGAATAATTTACCTACTGTCATGATTAATGC
GCTGGCAATCGATCATACATCTTATAGTGGAATAATGAAAGACGCGCTGGTTTATGCGAATGTCATTGGCTCTGACTTAG
GACCGAAAATAACACCAATTGGTTCGCTTGCTACATTGCTTTGGCTACATGTCTTGGCTCAGAAAGGCATAAAAATAAGC
TGGGGTTCCTATTTTAAAATTGGAATTGTCATAACTATTCCAGTTTTATTTGTTACACTTGTTGGTCTTTATATTTCTTT
ACTCATATGGAACTAA

Upstream 100 bases:

>100_bases
CGAGTTTTTTTATGTGTGATTGGAAATTAAATGTTTGATGTTTGATGGATAGAGGCATATATTTATAGTTGAATTATATA
AGCTAGAGAAAGGGGAAGTT

Downstream 100 bases:

>100_bases
AAAAACACCCTGTACATAATGTGCAGGGTGTTTACATTTTATAAAGAACATGTGGTTTTAAAGGGTCACCATCAATTAAT
GCTGGATGTTCAAAAGTAGC

Product: arsenic efflux pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQITLAVLVFAMTLLFVIWQPRNLSIGWSACCGALIALLLGVVTLSDVVTVTGIVWNATLAFIAIIIISLILDEIGFFEW
AALHMARIARGNGLLMFILISVLGAFVAAFFANDGAALILTPIVLAMVRALHFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFHISFSKYALIMWIPTLFSLIASIVILYIYFRKALPRKYDASDIQVPSSALKDKKMFRISWGILIVLVIGY
FSSSFLEIPVSFIAILVALIFLLVGAKSHAVSTKSILKGAPWNIVFFSVGMYVVVYGLQNAGITKLLSEAISYVSNYGLF
IGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSYSGIMKDALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFVTLVGLYISLLIWN

Sequences:

>Translated_431_residues
MQITLAVLVFAMTLLFVIWQPRNLSIGWSACCGALIALLLGVVTLSDVVTVTGIVWNATLAFIAIIIISLILDEIGFFEW
AALHMARIARGNGLLMFILISVLGAFVAAFFANDGAALILTPIVLAMVRALHFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFHISFSKYALIMWIPTLFSLIASIVILYIYFRKALPRKYDASDIQVPSSALKDKKMFRISWGILIVLVIGY
FSSSFLEIPVSFIAILVALIFLLVGAKSHAVSTKSILKGAPWNIVFFSVGMYVVVYGLQNAGITKLLSEAISYVSNYGLF
IGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSYSGIMKDALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFVTLVGLYISLLIWN
>Mature_431_residues
MQITLAVLVFAMTLLFVIWQPRNLSIGWSACCGALIALLLGVVTLSDVVTVTGIVWNATLAFIAIIIISLILDEIGFFEW
AALHMARIARGNGLLMFILISVLGAFVAAFFANDGAALILTPIVLAMVRALHFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFHISFSKYALIMWIPTLFSLIASIVILYIYFRKALPRKYDASDIQVPSSALKDKKMFRISWGILIVLVIGY
FSSSFLEIPVSFIAILVALIFLLVGAKSHAVSTKSILKGAPWNIVFFSVGMYVVVYGLQNAGITKLLSEAISYVSNYGLF
IGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSYSGIMKDALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFVTLVGLYISLLIWN

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=423, Percent_Identity=56.7375886524823, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46817; Mature: 46817

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLAVLVFAMTLLFVIWQPRNLSIGWSACCGALIALLLGVVTLSDVVTVTGIVWNATL
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIAIIIISLILDEIGFFEWAALHMARIARGNGLLMFILISVLGAFVAAFFANDGAALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TPIVLAMVRALHFDEKKVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFHISFSKYALI
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHH
MWIPTLFSLIASIVILYIYFRKALPRKYDASDIQVPSSALKDKKMFRISWGILIVLVIGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
FSSSFLEIPVSFIAILVALIFLLVGAKSHAVSTKSILKGAPWNIVFFSVGMYVVVYGLQN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHCCC
AGITKLLSEAISYVSNYGLFIGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSYSGIMK
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
DALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKISWGSYFKIGIVITIPVLFVTL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHH
VGLYISLLIWN
HHHHHHHHHCC
>Mature Secondary Structure
MQITLAVLVFAMTLLFVIWQPRNLSIGWSACCGALIALLLGVVTLSDVVTVTGIVWNATL
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIAIIIISLILDEIGFFEWAALHMARIARGNGLLMFILISVLGAFVAAFFANDGAALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TPIVLAMVRALHFDEKKVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFHISFSKYALI
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHH
MWIPTLFSLIASIVILYIYFRKALPRKYDASDIQVPSSALKDKKMFRISWGILIVLVIGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
FSSSFLEIPVSFIAILVALIFLLVGAKSHAVSTKSILKGAPWNIVFFSVGMYVVVYGLQN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHCCC
AGITKLLSEAISYVSNYGLFIGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSYSGIMK
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
DALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKISWGSYFKIGIVITIPVLFVTL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHH
VGLYISLLIWN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]