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The map label for this gene is glnA [H]

Identifier: 116872730

GI number: 116872730

Start: 1305757

End: 1307091

Strand: Direct

Name: glnA [H]

Synonym: lwe1314

Alternate gene names: 116872730

Gene position: 1305757-1307091 (Clockwise)

Preceding gene: 116872729

Following gene: 116872731

Centisome position: 46.4

GC content: 37.83

Gene sequence:

>1335_bases
ATGGCAAAATATACAAAAGAAGACATTTTCCGCTTCGCAGATGAACAGAATGTAAAATTTATTCGTTTACAGTTCACGGA
TATTCTCGGGATAATTAAGAATGTGGAAATTCCTGTTAGCCAACTAAAGAAAGCACTAGATAACAAAATCATGTTTGATG
GTTCCTCCATAGAAGGTTTTGTGAGAATTGAAGAGTCCGATATGTATTTATTCCCGGACCTCGACACTTGGGTAGTATTC
CCTTGGACAGCTGAAAAAGGAAAAGTTGCAAGAATGATTTGTGATATTTATAATCCTGATATGACACCATTTGCAGGAGA
TCCGCGTGCAAACTTAAAACGTGTACTTAAAGAAATGGAAGAGTTAGGTTTCACGGAATTTAACTTAGGACCAGAACCAG
AATTCTTCCTCTTCAAATTAGATGAAAATCGTCGTCCGACACTTGAACTAAATGATAGTGGTGGTTACTTCGATTTAGCA
CCAACTGATTTAGGCGAAAACTGCCGACGTGATATTGTGCTTGAATTAGAAGAAATGGGCTTTGAAATCGAAGCATCCCA
TCATGAAGTGGCTCCTGGTCAACATGAAATTGACTTTAAATATGAAGATGCAATTACGGCGTGTGATAGTATCCAAACAT
TTAAATTAGTAGTTAAAACAATTGCTCGGAAACATGGTTTGCATGCTACATTTATGCCAAAACCACTATTTGGAGTAAAT
GGTTCAGGAATGCATTTCAATATGTCCTTATTTACCGAAGAAGGTAATGCGTTTTATGATAAAGATGGTGAACTTGAATT
AAGCCAAACAGCTTATCACTTCTTAGCAGGTATGTTAAAACACGCTCGTGGATTTACAGCGGTAACTAACCCAACAATCA
ATTCTTTTAAACGTTTAGTTCCTGGTTATGAAGCACCATGTTACATTGCATGGTCAGGTAAAAACCGTAGTCCACTTGTG
CGCGTACCAAGTTCTCGTGGACTTAGTACTCGTCTTGAATTACGTAGTGTAGATCCATCTGCTAATCCATACTTAGCAAT
GGCAGTATTACTAAAAGCTGGTTTATCTGGAATTAAAGATGAATTAACTCCTCCAGCACCGGTTGATCGTAATATTTATG
GTATGAACGAAGAAGAACGTGAAGCGACTGGTATTTACGATCTTCCAGAAAGCCTAGGTCACGCGCTAATTGAGCTTGGA
AAAGATGATACCATTAGAGACGGTTTAGGTGAACATATTTTTGAACACTTTATTGAAGCAAAAACGATTGAATGTGATAT
GTTCCGTACAGCGGTTCATCCGTGGGAACGCGAACAATATCTTGAAATCTATTAA

Upstream 100 bases:

>100_bases
ACAGGAAAACAACAATTACCAAGGTTTTAAAAAAACTAGAAGAGGTATTTACTTTATTTAGAAGTTAATATAATTAGATT
ATTTAGGAGGAATTTAGATT

Downstream 100 bases:

>100_bases
TACTAAAAAAATCTCGTTTCCTATTATTGGAAGCGAGTTTTTTTATGTGTGATTGGAAATTAAATGTTTGATGTTTGATG
GATAGAGGCATATATTTATA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVF
PWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAITACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHFNMSLFTEEGNAFYDKDGELELSQTAYHFLAGMLKHARGFTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLV
RVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELG
KDDTIRDGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY

Sequences:

>Translated_444_residues
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVF
PWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAITACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHFNMSLFTEEGNAFYDKDGELELSQTAYHFLAGMLKHARGFTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLV
RVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELG
KDDTIRDGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY
>Mature_443_residues
AKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVFP
WTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAP
TDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAITACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVNG
SGMHFNMSLFTEEGNAFYDKDGELELSQTAYHFLAGMLKHARGFTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLVR
VPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELGK
DDTIRDGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=458, Percent_Identity=25.3275109170306, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=37.9454926624738, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI87081870, Length=458, Percent_Identity=29.0393013100437, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50479; Mature: 50348

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGF
CCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLFPDLDTWVVFPWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYEDAITACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHFNMSLFTEEGNAFYDKDGELELSQTAYHFLAGMLKHARGFTAVTNPTINSFKRLV
CCCEEEEEEEEEECCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHC
PGYEAPCYIAWSGKNRSPLVRVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKD
CCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELGKDDTIRDGLGEHIFEHFIEA
CCCCCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
KTIECDMFRTAVHPWEREQYLEIY
CCEEHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
AKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGF
CCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLFPDLDTWVVFPWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYEDAITACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHFNMSLFTEEGNAFYDKDGELELSQTAYHFLAGMLKHARGFTAVTNPTINSFKRLV
CCCEEEEEEEEEECCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHC
PGYEAPCYIAWSGKNRSPLVRVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKD
CCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELGKDDTIRDGLGEHIFEHFIEA
CCCCCCCCCCCEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
KTIECDMFRTAVHPWEREQYLEIY
CCEEHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]